Evaluation of entomopathogenic nematodes for the management of Phyllophaga bicolor (Coleoptera: Melolonthidae)
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Scarab larvae (Coleoptera: Melolonthidae) are important pests in a diversity of crops and climates owing to their damage to the quality and yield of agricultural products. An alternative for their biological control are the entomopathogenic nematodes, generalist parasites and effective control agents in laboratory and field trials. Isolates of introduced and native entomopathogenic nematodes were evaluated against third instar larvae of Phyllophaga bicolor. We determined the infectivity and mortality of isolates of Steinernema (S. riobravis-Sr, S. carpocapsae Strain all-Sc, Steinernema sp.-SNI, S. arenarium-Sa and S. feltiae-Sf1 and Sf2) and Heterorhabditis (H. bacteriophora-Hb1, Hb2 and Hb3) at a concentration of 10,000 IJs/ml at 23C and 70 ± 5% R.H. The evaluation was done on larvae that were one, two and three months old. The most ineffective isolates were those of Heterorhabditis (93.75%-Hb2; 89.58% and 64.58%-Hb1-Hb3), compared to those of Steinernema (10.42%-SIN; 12.50%-Sr; 16.67%-Sa, 22.92%-Sc; 33.34%Sf2; 66.67%-Sf1). The lowest mortalities resulted from Steinernema isolates (0%-Sc and SNI; 2.08%-Sr, 6.25%-Sa; 6.25%-Sf2 and 47.92%-Sf1) while the highest were from Heterorhabditis (52.09%-Hb3; 72.92% and 91.67%-Hb1Hb2). Differences were noted in infection (1 = 65.64%, 2 = 42.50%, 3 = 29.17%) and mortality (1 = 53.33%, 2 and 3 averaged 31.46%), depending on larval maturity, with a noticeable reduction in these variables when approaching the prepupa. The genus Heterorhabditis is the most promising for the white grub management, especially when applied to young larvae.
CIAT. 2004. Soil pests-Cassava and other crops, pp. 116-165. En: Centro Internacional de Agricultura Tropical (CIAT). Annual Report. Integrated pest and disease management in major agroecosystems. Cali, Colombia. 417 p.
FORSCHLER, B. T.; GARDNER, W. A. 1991. Concentration-mortality response of Phyllophaga hirticula (Coleoptera: Scarabaeidae) to three entomogenous nematodes. Journal of Economic Entomology 84 (3): 841-843.
GAUGLER, R.; CAMPBELL, J. F.; SELVAN, S.; LEWIS, E. E. 1992. Large-scale inoculative releases of entomopathogenic nematode Steinernema glaseri: assessment 50 years later. Biological Control 2: 181-187.
GAUGLER, R.; WANG, Y.; CAMPBELL, J. F. 1994. Aggressive and evasive behaviours in Popillia japonica (Coleoptera: Scarabaeidae) larvae: defences against entomopathogenic nematode attack. Journal of Invertebrate Pathology 64: 193199.
GEORGIS, R.; GAUGLER, R. 1991. Predictability in biological control using entomopathogenic nematodes. Journal of Economic Entomology 84: 713-720.
GLASER, R. 1932. Studies on Neoaplectana glaseri, a nematode parasite of the Japanese beetle (Popillia japonica). New Jersey Department of Agriculture. Circular 211. Trenton, NJ. 34 p.
INFOSTAT. 2009. Manual del usuario. Grupo INFOSTAT, FCA., Universidad Nacional de Córdoba. 1 Ed. Editorial Brujas, Argentina. 318 p.
JACKSON, T. A. 1993. Developing microbial controls for scarab pest. En: Diversidad y manejo de plagas subterráneas. Memorias de la IV mesa redonda sobre plagas subterráneas 14 y 15 de Octubre, Instituto de Ecología, AC. Xalapa, Veracruz, México. Sociedad Mexicana de Entomología, AC. Instituto de Ecología. AC. 261 p.
JACKSON, J. J.; BROOKS, M. A. 1995. Parasitism of western corn rootworm larvae and pupae by Steinernema carpocapsae. Journal of Nematology 27 (1): 15-20.
KAYA, H. K. 1985. Susceptibility of early larval stages of Pseudalletia unipuncia and Spodoptera exigua (Lepidoptera: Noctuidae) to the entomogenous nematode Steinernema feltiae (Rhabditida: Steinernematidae). Journal of Invertebrate Pathology 46: 58-62.
KLEIN, M. G. 1990. Efficacy against soilinhabiting insects pests, pp. 195-214. En: Gaugler, R.; Kaya, H. K. (Eds.), Entomopathogenic nematodes in biological control. CRC. Press, Boca Raton, Florida. 365 p.
KLEIN, M. G. 1992. Use of Bacillus popilliae in Japanese beetle control, pp. 179190. En: Jackson, T. A.; Glare, T. R. (Eds.). Use of pathogens in scarab pest management. Intercept Andover. Hampshire, England. 298 p.
KLEIN, M. G. 1993. Biological control of scarabs with entomopathogenic nematodes, pp. 4957. En: Bedding, R.; Akhurst, R.; Kaya, H. (Eds.). Nematodes and the biological control of insects. CSRIO, East Melbourn.
KOPPENHÖFER, A. M.; FUZY, E. M. 2004. Effect of white grubs developmental stage on susceptibility to entomopathogenic nematodes. Journal of the Entomological Society of America 97 (6): 1842-1849.
KOPPENHÖFFER, A. M.; FUZY, E. M.; CROCKER, R. L.; GELERNTER, W. D.; POLAVAPARU, S. 2004. Pathogenicity of Heterorhabditis bacteriophora, Steinernema glaseri, and S. scarabaei (Rhabditida: Heterorhabditidae, Steinernematidae) against 12 white grubs species (Coleoptera: Scarabaeidae). Biocontrol Science and Technology 14 (1): 87-92.
LACEY, L. A.; BETTENCOURT, R.; GARRETT, F. J.; SIMÕES, N. J.; GAUGLER, R. H. 1993. Factors influencing parasitism of adult Japanese beetles, Popillia japonica (Col.: Scarabaeidae) by entomopathogenic nematodes. Entomophaga 38 (4): 501509.
LONDOÑO, M. 2001. Las chizas y su manejo, hortalizas, plagas y enfermedades. Corpoica-Sociedad Colombiana de Entomología. Rionegro. p. 36-47.
MELO, E. L.; ORTEGA, C. A.; GAIGL, A.; KOPPENHÖFER, A.; BELLOTTI, A. C. 2006. Evaluación de patogenicidad e infección de la nueva especie Steinernema scarabaei Stock & Koppenhöfer (Rhabditida: Steinernematidae), sobre la chisa rizófaga Phyllophaga pos. Bicolor, pp. 127-136. En: Parada, S. J.C.; Luque, J. E.; Piedrahita, C. W. de J. (Eds.). Nematodos entomoparásitos: experiencias y perspectivas. Editorial Unibiblos. 193 p.
MELO, E. L.; ORTEGA-OJEDA, C. A.; GAIGL, A. 2007. Efecto de nematodos sobre larvas de Phyllophaga menetriesi y Anomala inconstans (Coleoptera: Melolonthidae). Revista Colombiana de Entomología 33(1): 21-26.
ORTEGA, C. A. 2005. Estudios metodológicos para evaluar el impacto económico de escarabajos Melolonthidae (Insecta: Coleoptera) en tres cultivos tropicales. Trabajo de grado de Master en Ciencias en Fitoprotección. Escuela Politécnica del Ejército. Quito, Ecuador. 109 p.
PARDO-LOCARNO, L. C. 2002. Aspectos sistemáticos y bioecológicos del complejo chisa (Col, Melolonthidae) de Caldono, Norte del Cauca, Colombia. Trabajo de grado de Maestría en Biología. Universidad del Valle, Facultad de Ciencias, Santiago de Cali. 145 p.
PETERS, A. 1996. The natural host range of Steinernema and Heterorhabditis spp. and their impact on insects populations. Biocontrol Science and Technology 6: 389-402.
POSADA, O. L. 1993. Las chisas, sus enemigos naturales y recomendaciones sobre su manejo. Agricultura Tropical 30 (3): 7179.
RESTREPO-GIRALDO, H.; LÓPEZ-ÁVILA, A. 2000. Especies de chisas (Coleoptera: Melolonthidae) de importancia agrícola en Colombia. Corporación Colombiana de Investigación Agropecuaria. Programa Nacional de Manejo Integrado de Plagas. 62 p.
SHANNON, P. J. 1994. Control microbiano de Phyllophaga spp. (Col: Melolonthidae),pp. 80-92. En: Memoria Seminario Taller sobre la biología y control de Phyllophaga spp. Informe Técnico No. 277. CATIE. Turrialba, Costa Rica.
SHANNON, P.; CARBALLO, M. 1996. Biología y control de Phyllophaga spp., pp. 80-93. En: Seminario-Taller Centroamericano sobre la biología y control de Phyllophaga spp. Serie Técnica. Informe Técnico 227. CATIE, Turrialba, Costa Rica.
SHAPIRO-ILAN, D. I.; GOUGE, D. H.; KOPPENHÖFER, A. M. 2002. Factors affecting commercial success: case studies in cotton, turf and citrus, pp. 333–355. En: Gaugler, R. (Ed.). Entomopathogenic nematology. CAB International. Wallingford.
VILLANI, M. G.; WRIGHT, R. J. 1988. Entomogenous nematodes as biological control agents of European chafer and Japanese beetle (Coleoptera: Scarabaeidae) larvae infesting turfgrass. Journal of Economic Entomology 81 (2): 484-487.
- Miguel Mendieta, Andreas Gaigl, Juan Carlos Getiva de la Hoz, Anibal Orlando Herrera, Preferencias alimenticias y respuestas de olfato de Copitarsia uncilata (Lepidoptera: Noctuidae) a cuatro especies de hierbas aromáticas , Revista Colombiana de Entomología: Vol. 43 No. 2 (2017)
- ELSA LILIANA MELO-M., CARLOS ALBERTO ORTEGA-O., ALPER SUSURLUK, ANDREAS GAIG, ANTHONY C. BELLOTTI, Native entomopathogenic nematodes (Rhabditida) in four departments of Colombia , Revista Colombiana de Entomología: Vol. 35 No. 1 (2009)
- MA. DEL PILAR HERNÁNDEZ, ANTHONY BELLOTTI, CESAR CARDONA, STEPHEN LAPOINTE, ALBERTO PANTOJA, Organization and utility of a collection of insects for reference and work in four tropical crops , Revista Colombiana de Entomología: Vol. 21 No. 1 (1995)
- Miguel Mendieta, Andreas Gaigl, Juan Carlos Getiva De La Hoz, Anibal Orlando Herrera, Preferencias alimenticias y respuestas de olfato de Copitarsia uncilata (Lepidoptera: Noctuidae) a cuatro especies de hierbas aromáticas , Revista Colombiana de Entomología: Vol. 44 No. 1 (2018)
- ELSA LILIANA MELO-M., CARLOS ALBERTO ORTEGA-OJEDA, ANDREAS GAIGL, RALF-UDO EHLERS, ANTHONY C. BELLOTTI, Evaluation of two commercial strains of entomonematodes as control agents of Cyrtomenus bergi Froeschner (Hemiptera: Cydnidae) , Revista Colombiana de Entomología: Vol. 32 No. 1 (2006)
- OSCAR SANTOS A., EDGAR HERNEY VARÓN D., ANDREAS GAIGL, ANDREA FLORIANO, Economic injury level for Neohydatothrips signifer (Thysanoptera: Thripidae) in passion fruit at the Huila region, Colombia , Revista Colombiana de Entomología: Vol. 38 No. 1 (2012)
- NELLY PATRICIA VILLEGAS, ANDREAS GAIGL, LUIS FERNANDO VALLEJO-E., The white grub complex (Coleoptera: Melolonthidae) associated with onion and pasture in Risaralda, Colombia , Revista Colombiana de Entomología: Vol. 34 No. 1 (2008)
- ELSA LILIANA MELO-MOLINA, CARLOS ALBERTO ORTEGA-OJEDA, ANDREAS GAIGL, The effect of nematodes on larvae of Phyllophaga menetriesi and Anomala inconstans (Coleoptera: Melolonthidae) , Revista Colombiana de Entomología: Vol. 33 No. 1 (2007)
- CARLOS ALBERTO ORTEGA-OJEDA, ELSA LILIANA MELO-MOLINA, ANDREAS GAIGL, Lethal density of Phyllophaga menetriesi (Coleoptera: Melolonthidae) on cassava plants (Manihot esculenta) , Revista Colombiana de Entomología: Vol. 33 No. 1 (2007)
- ANTHONY BELLOTTI, JOSÉ MARÍA GUERRERO, VARIETAL RESISTANCE IN YUCCA AGAINST MITES Trtranychus urticae and Mononychellus tanajoa , Revista Colombiana de Entomología: Vol. 3 No. 3-4 (1977)
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