Effect of culture conditions on the entomopathogenic activity of Serratia marcescens against Tocia solanivora (Lepidoptera: Gelechiidae)
Main Article Content
A rnodel was established to evaluate the effect of successive inoculations and successive growths in culture media supplernented or not with potential virulence inductors (wheat bran and hornogenised Tecia so/anivora Povolny larvae) on the enzymatic and biocontrol activity of Serratia marcescens. The bacteria biornass obtained frorn these media was evaluated for biocontrol activity against insect larvae, and enzyrnatic extracts were prepared to deterrnined the activity of the enzymes N-acetilglucosarninidase, quirnoelastase protease PR-1 and B-esterase. These activities were evaluated in each of five successive bacteria cultures. In general, both the type of substrate added to the media as well as successive growth affected the biocontrol and enzyrnatic activities, increasing in the third growth of the bacteria. The greatest biocontrol activities of S. marcescens, with or without activation in larvae, corresponding to 65 and 64% rnortality, respectively, were obtained when were the bacteria were grown in wheat bran rnediurn, followed by 58 and 59% rnortality, respectively, when grown in the larval rnediurn, activities that were significantly different than that obtained when S. marcescens grew in medium without supplernent (48,5%). In addition, a positive correlation was found between N-acetilglucosarninidase and quimoelastase protease activities and S. marcescens biocontrol activity, suggesting that the activity of these enzyrnes could be related with the rnode of action of entomopathogenis bacteria of he genus Serratia.
BALAKRISHNANH. .; SRINIVASANM, .; RELE,M. 1997. Extracellular protease activities in relation to xylanase secretion in an alkalophilic Bacillus sp. Biotechnology Letters 19 (7): 599-60 1.
BISWAS, S.; MISHRA, A.; NANDA, G. 1988. Xylanase and B-xylosidase production by Aspergillus ochraceus during growth on lignocelluloses. Biotechnollogy Bioenginering 31: 613-616.
BOSA, C. 2001. Efecto de la composición del medio de cultivo y los pases sucesivos sobre la actividad enzimática y biocontroladora de Serratia marcescens hacia Tecia solanivora Povolny (Lepidoptera: Gelechiidae). Tesis de Maestríaen CienciasAgrarias. Universidad Nacional de Colombia, Facultad de Agronomía, Bogotá. 111 p.
BOSA, C.F: COTES, A. M. 2004. Efecto de las condiciones de cultivo sobre la actividad enzimática de Serralia marcescens contra Tecia solanivora (Lepidoptera: Gelechiidae). Revista Colombiana de Entomología 30 (1): 79-85.
CALLAGHAN, M.; GARNHAM, L.; NELSON, T.; BAIRD, D.; JACKSON, T. 1996. The Pathogenicity of Serratia strains to Lucilia sericata (Diptera: Calliphoridae). Journal of Invertebrate Pathology 68 (1): 22-27.
CIBA-GEIGY.1978.Manualde ensayos de campo. Cómo realizar un ensayo biológico. Bogotá. p. 14-18.
DANCER,B.; CHANTAWANNAKUL P,. 1997. The proteases of American foulbrood scales. Journal of Invertebrate Pathology 70: 7987.
EWING, W.; MARTIN, W. 1981. Enterobacteriaceas. En: Manual de Microbiología Clínica. Cap 18, 2da edición. Barcelona, España. p. 192-223.
FLACH, J; PILET, P.; JOLLES, P. 1992. Whars new in chitinase research? Experientia 48: 701-716.
FLYG, C.; KERSTlN, K.; BOMAN, N. 1980. Insect pathogenic properties of Serratia marcescens: Phage-resistant mutans with a decreased resistance to Cecropia Immunity and a decreased virulence to Drosophila. Journal of General Microbiology 120: 173181.
FOX, C.: JAQUES, P. 1958. Note on the greenmuscardine fungus Metarhizium anisopliae (Metsch) as a control for wireworms. Canadian Entomology 90: 314-315.
GODFRAY, N.; BRIGGS, C.; BARLOW, N.; CALLAGNAN, M.; GLARE, T.; JACKSON, T. 1999. A model of insect-pathogen dynamics in which a pathogenic bacterium can also reproduce saprophytically. Proceddings RoyaI Society of London 266: 233-240.
GONZÁLES, M.; VALENCIA, A.; BUSTILLO, A. 1999. Incremento de la patogenicidad de B. bassiana hacia Hypothenemus hempei, utilizando cutícula de broca en el medio de cultivo. Resúmenes XXVICongreso Sociedad Colombiana de Entomología, Bogotá. 156 p.
GOODAY, G. 1994. Physiology of microbial degradation of chitin and chitosan. Biochemistry of Microbial Degradation p. 279-312.
GRAY, D. 1998. Sex DitTerencesin Susceptibility of house Crickets, Acheta domesticus, to experimental Infection with Serratia liquefaciens. Journal of Invertebrate Pathology 71(3):288-289.
GRKOVIC, S.; GLARE, T.; TREVOR, A.; CORBETT, G. 1995. Genes essential for amber disease in grass grubs are located on the large plasmid found in S. entomophila and S. proteamaculans. Applied and Environmental Microbiology 51 (6): 2218-2223.
HAWTIN, R.; ZARKOWSKA T, ARNOLD, K.;THOMAS, C. 1997. Liquefaction of Autographa californica Nucleopolyhedrovirus infected insects is dependent on the integrity of virus encoded chitinase and cathepsin genes. Virology 238: 243-253.
KHOURY, C.: MINIER, M.; HUYNN, N.; GOFFIC, F. 1997. Optimal dissolved oxygen concentration for the production of chitinases by S. marcescens. Biotechnology Letters 19 (11): ll43-1146.
LANE, B.; TRINCI, A. 1991. Influence of cultural conditions on the virulence of conidia and blastospores of Beauveria bassiana to the green leafhopper Nephotettix virescens. Mycologycal. Research 95 (7): 829-833.
RABELO, F.; MOREIRA, W.; SANTOS,G. 1985. Efeito de sucesivas repicagens em arroz na virulencia de Metarhizium anisopliae para Deois flavopicta. Pesquisa Agropecuaria. Brasilia 20 (10): 1115-1118.
SAMPSON, M.; GOODAY, G. 1998. Involvement of chitinases of Bacillus thuringiensis during pathogenesis in insects. Microbiology 144: 2189-2194.
SCNAERFFENBERG, B. 1964. Biological and environmental conditions for the development of mycoses caused by Beauveria sp.. And Metarhizium sp. Journal of Invertebrate Pathology 6:8-20.
VILLAMIZAR, 1. 1998. Efecto de la composición del medio de cultiven la virulencia de Metarhizium anisopliae sobre la langosta Llanera Rhammatocerus schistocercoides. Tesis de Maestría para optar al título de Microbióloga. Universidad Nacional de Colombia. Bogotá. I 12 p.
- LAURA VILLAMIZAR, CAROLINA ARRIERO, CARLOS FELIPE BOSA-O., ALBA MARINA COTES, Development of preformulated products based on Nomuraea rileyi for control of Spodoptera frugiperda (Lepidoptera: Noctuidae) , Revista Colombiana de Entomología: Vol. 30 No. 1 (2004)
- CARLOS FELIPE BOSA-O., DORA CHÁVEZ, LISSETTE TORRES, ALEJANDRO PARÍS, LAURA VILLAMIZAR, ALBA MARINA COTES, Evaluation of native isolates of Nomuraea rileyi for the control of Spodoptera frugiperda (Lepidoptera: Noctuidae) , Revista Colombiana de Entomología: Vol. 30 No. 1 (2004)
- CARLOS ESPINEL-C., MARÍA DENIS LOZANO, LAURA VILLAMIZAR-R., ERIKA GRIJALBA-B., ALBA MARINA COTES-P., IPM strategy for the control of Bemisia tabaci (Hemiptera: Aleyrodidae) on melon and tomato , Revista Colombiana de Entomología: Vol. 34 No. 2 (2008)
- MARÍA DEL PILAR PACHÓN, ALBA MARINA COTES-P., Effect of Verticillium lecanii entomopathogen of the Whitefly Trialeurodes vaporariorum on the parasitic activity of Amitus fuscipennis in a bean culture , Revista Colombiana de Entomología: Vol. 23 No. 2 (1997)
- NATALIA AGUIRRE, LAURA VILLAMIZAR-R., CARLOS ESPINEL-C., ALBA MARINA COTES-P., Effect of pH and water activity over Nomuraea rileyi (Hyphomycetes) development , Revista Colombiana de Entomología: Vol. 35 No. 2 (2009)
- FELIPE BOSA-O., PABLO OSORIO-M., ALBA MARINA COTES-P., MARIE BENGTSSON, PETER WITZGALL, TAKEHIKO FUKUMOTO, Control of Tecia solanivora (Lepidoptera: Gelechiidae) through its pheromone for mating disruption , Revista Colombiana de Entomología: Vol. 34 No. 1 (2008)
- PAOLA CUARTAS-O., LAURA VILLAMIZAR-R., CARLOS ESPINEL-C., ALBA MARINA COTES-P., Infection of native granulovirus on Tecia solanivora and Phthorimaea operculella (Lepidoptera: Gelechiidae) , Revista Colombiana de Entomología: Vol. 35 No. 2 (2009)
- CARLOS FELIPE BOSA-O., PETER WITZGALL, MARIE BENGTSSON, ALBA M. COTES-P., Biological characterization of the sex pheromone compounds Tecia solanivora Povolny (Lepidoptera: Gelechiidae) , Revista Colombiana de Entomología: Vol. 31 No. 2 (2005)
- ERIKA PAOLA GRIJALBAB, LAURA VILLAMIZAR-R., ALBA MARINA COTES-P., Evaluation of the stability of Paecilomyces sp. and Beauveria bassiana under ultraviolet radiation , Revista Colombiana de Entomología: Vol. 35 No. 1 (2009)
- CARLOS FELIPE BOSA-O., ALBA MARINA COTES-P., Effect of culture conditions on the enzymatic activity of Serratia marcescens against Tecia solanivora (Lepidoptera: Gelechiidae) , Revista Colombiana de Entomología: Vol. 30 No. 1 (2004)
Authors retain the copyright on their work and are responsible for the ideas expressed in them. Once a manuscript is approved for publication, authors are asked for a publication license for the term of legal protection, for all territories that allows the use, dissemination and disclosure of the same.