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Two biopesticide prototypes were developed, formulated as wettable powders by using two native isolates of Paecilomyces sp. and Beauveria bassiana previously selected for their high biocontrol activity on Bemisia tabaci. Considering that ultraviolet (UV) radiation is one of the most limiting factors when microorganisms are applied under field conditions, the aim of this study was to determine the stability of formulated and unformulated conidia of these fungi when exposed to UV light. Germination of the microorganisms was evaluated in malt extract agar before and after three, six and 24 hours of exposure to UV “C” radiation, under laboratory conditions. In addition, the effect of solar radiation under field conditions was evaluated during six hours of direct exposure to sun in the municipality of El Espinal (Tolima). The UVC radiation did not affect germination of unformulated Paecilomyces sp. conidia, while solar radiation reduced germination by 43 and 42% for unformulated and formulated conidia, respectively. UVC and solar radiation reduced germination of unformulated B. bassiana conidia by 43 and 46%, respectively. For formulated conidia, solar radiation reduced germination by 26%. The evaluated formulation protected conidia of B. bassiana against solar radiation, but not those of Paecilomyces sp., making it necessary for the latter to evaluate sunscreens that might be included in the formulation.

GRIJALBAB, E. P., VILLAMIZAR-R., L., & COTES-P., A. M. (2009). Evaluation of the stability of Paecilomyces sp. and Beauveria bassiana under ultraviolet radiation. Revista Colombiana De Entomología, 35(1), 1–6. https://doi.org/10.25100/socolen.v35i1.9179

AIKENS, J.; DIX, T. 1991. Perhydroxyl radical (HOO.) initiated lipid peroxidation: the role of fatty acid hydroperoxides. Journal of Biology and Chemistry (266): 15091-15098.

BRAGA, G.; FLINT, S.; MESSIAS, C.; ANDERSON, A.; ROBERTS, D. 2001. Variability in response to UV B among species and strains of Metarhizium isolated from sites at latitudes from 61º N to 54º S. Journal of Invertebrate Pathology 78: 98-108.

BRAGA, G.; RANGEL, D.; FLINT, S.; ANDERSON, A.; ROBERTS, D. 2006. Conidial pigmentation is important to tolerance against solar-simulated radiation in the entomopathogenic fungus Metarhizium anisopliae. Photochemistry and Photobiology (82): 418-422.

BRITT, A. B.; 1996. DNA damage and repair in plants. Annual Reviews in Plant Physiology and Plant Molecular 47: 75-100.

BUSSNINK, H. J.; OLIVER, R. 2001. Identification of two highly divergent catalase genes in the fungal tomato pathogen, Cladosporium fulvum. European Journal of Biochemistry 268: 15-24.

CERDA-OLMEDO, E.; ROJAS, M.; CUBERO, B. 1996. Causes of cell death following ultraviolet B and C exposures and the role of carotenes. Photochemical Photobiology 64: 547-551

CHARY, P.; NATVIG, D. 1989. Evidence for three differentially regulated catalase genes in Neurospora crassa: effects of oxidative stress, heat shock and development. Journal of Bacteriology 171: 2646-2652.

CHOE, M.; JACKSON, C.; YU, B. 1995. Lipid peroxidation contributes to age-related membrane rigidity. Free Radical Biology Medical 18: 977-984.

DEVOTTO, L.; GERDING, M. 2003. Respuesta de dos aislamientos chilenos de Metarhizium anisopliae (Metschnikoff) sorokin a la adición de un protector solar. Agricultura Técnica 63: 339-346.

DIFFEY, B. 1991. Solar ultraviolet radiation effects on biological systems. Physics in Medicine and Biology 36: 299-328.

EYAL, J.; MABUD, M.; FISCHBEIN, K.; WALTER, J.; OSBORNE, L.; LANDA, Z. 1994. Assessment of Beauveria bassiana Nov. EO-1 strain, which produced a red pigment for microbial control. Applicated Biochemistry and Biotechnology 44: 65-80.

FRIEDBERG, E. C.; WALKER, G. C.; SIEDE, W. 1995. DNA repair and mutagenesis. American Society for Microbiology. Washington, D. C. 698 p.

GUTTERIDGE, J.; HALLIWELL, B. 1990. The measurement and mechanism of lipid peroxidation in biological systems. Trends Biochemistry Science 15: 129-135.

HULLO, M., MOSZER, I.; DANCHIN, A.; MARTIN, I. 2001. CotA of Bacillus subtilis is a copper dependent laccase. Journal of Bacteriology 183: 5426-5430.

IDEAM; UPME 2005. Atlas de Radiación Solar de Colombia. Instituto de meteorología, hidrología y estudios ambientales; Unidad de planeación minero energética (eds.) Bogotá, Colombia 175 p.

INSTITUTO GEOGRÁFICO AGUSTÍN CODAZZI. 1996 Diccionario Geográfico de Colombia. Colombia. 2.504 p.

KAWASAKI, I.; AGUIRRE, J. 2001. Multiple catalase genes are differentially regulated in Aspergillus nidulans. Journal of Bacteriology 183: 1434-1440.

KIELBASSA, C.; EPE, B. 2000. DNA damage induced by ultraviolet and visible light and its wavelength dependence. Methods in Enzymology 319: 436-445.

KRUTMANN, J. 2001. New developments in photoprotection of human skin. Skin Pharmacology and Applicate Skin Physiology 14: 401-407.

LÓPEZ, A. 1994. Avance y perspectivas del control biológico de las moscas blancas. En: Memorias del seminario manejo integrado de mosca blanca y técnicas de aplicación de pesticidas. SOCOLEN. Bogotá, pp. 42-53.

LÓPEZ, A.; GARCÍA, J. 2000. Manejo integrado sostenible de moscas blancas como plagas y vectores de virus en los trópicos. 1. Reconocimiento, diagnóstico y caracterización de moscas blancas como plagas en el trópico alto de América Latina. Informe final. Convenio DANIDA-CORPOICA. CIAT. 43 p.

MCKENZIE, R.; BJÔRN, L.; BAIS, A.; ILYAS, M. 2003. Changes in biologically active ultraviolet radiation reaching the earth’s surface. Photochemical Photobiology Science 2: 5-15.

MILLER, CH; RANCEL, D.; BRAGA, G.; FLINT, S.; KWON, S.; MESSIAS, C.; ROBERTS, D.; ANDERSON, A. 2004. Enzyme activities associated with oxidative stress in Metarhizium anisopliae during germination, mycelial growth, and conidiation and in response to near-UV irradiation. Journal of Microbiology 50: 41-49.

MOORE, D.; BRIDGE, P. D.; HIGGINS, P.; BATEMAN, R.; PRIOR, C. 1993. Ultraviolet radiation damage to Metarhizium flavoviride conidia and the protection given by vegetable and mineral oils and chemical sunscreens. Annuary of Applicated Biology 122: 605-616.

MOORE, D.; HIGGINS, P. M.; LOMER, C. J. 1996. Effects of simulated and natural sunlight on the germination of conidia of Metarhizium flavoviride Gams and rozsypal and interactions with temperature. Biocontrol Science and Technology 6: 63-76.

MORLEY, D.; MOORE, D.; PRIOR, C. 1996. Screening of Metarhizium and Beauveria spp. spores with exposure to simulated sunlight and a range of temperatures. Mycological Research 100: 31-38.

MULERO, M. 2004. Efecto de la radiación ultravioleta (RUV) sobre los procesos de estrés oxidativo e inmunosupresión cutánea. Efecto protector de los filtros solares. Tesis doctoral. Facultad de Medicina. Universidad Rovira I Virgili. 174 p.

NICHOLSON, W.; SCHUERGER A.; SETLOW P. 2005. The solar UV environment and bacterial spore UV resistance: considerations for earth to Mars transport by natural processes and human spaceflight. Mutation Research 571: 249-264.

RAGAEI, M. 1999. Radiation protection of microbial pesticides. Journal of Applied Entomology 123: 381-384.

RIBERA, P.; PARADELO, C. 1997. El sol y la piel: Fotoprotección y filtros solares. Medicina Integral 30 (2): 64-71.

RIESENMAN, P.; NICHOLSON W. 2000. Role of the spore coat layers in Bacillus subtilis spore resistance to hydrogen peroxide, artificial UVC, UVB and solar UV radiation. Applied Environment Microbiology 66: 620-626.

SAXENE, D.; BEN-DOV, E.; MANSHEROB, R.; BARAK. Z.; BOUSSIBA, S.; ZARITSKY, A. 2002. A UV tolerant mutant of Bacillus thuringiensis spp. Kurstaki producing melanin. Microbiology 44: 25-32.

SERPONE, N.; DONDI, D.; ALBINI, A. 2007. Inorganic and organic UV filters: Their role and efficacy in sunscreens and suncare products. Inorganica Chimica Acta 360: 794-802.

SERRA, N. 1994. Fotoprotectores y bronceadores. Actualidad Dermatológica 33: 447-458.

SETLOW, P. 1988. Resistance of bacterial spores to ultraviolet light. Comments Molecular Cell Biology Biophysics 5: 253-264.

SHAH, P. A.; DOURO-KPINDOU, O. K.; SIDIBE, A.; DAFFE, C. O.; PAUUW, H.; LOMER, C. J. 1998. Effects of the sunscreen oxybenzone on field efficacy and persistente of Metarhizium falvoviride conidia against Kraussella amabile (Orthoptera: Acrididae) in Mali, West Africa. Biocontrol Science Technology 8: 357-364.

SMITS, N.; SINOQUET H. 2004. Fungal bioinsecticide survival in response to UVB in 3D digitized grapevine canopies: a simulation study, pp. 187-189. En: Godin, C. (ed). 4th International workshop on functional-structural plant models. 7-11 june 2004. Montpellier. France. 201 p.

THOMA, F. 1999. Light and dark in chromatin repair: repair of UV induced DNA lesions by photolyase and nucleotide excision repair. EMBO Journal 18: 6585-6598.

TOBAR, S.; VÉLEZ, P.; MONTOYA, E. 1996. Selección de aislamientos patogénicos de Beauveria bassiana y Metarhizium anisopliae por resistencia a la luz ultravioleta. Congreso de la Sociedad Colombiana de Entomología (SOCOLEN). Cartagena -Colombia. 17 19 de julio de 1996, p. 76.

VILLAMIZAR, L.; COTES, A.; GÓMEZ, M. 1997. Producción masiva y preformulación de Metarhizium spp. (Metschnicov) para el control biológico de la langosta llanera Rhammatocerus schistocercoides (Rhen). Revista Colombiana de Entomología 23 (3-4): 119-124.

YOUNG, A.; POTTE, C.; NIKAIDO, O.; PARSONS, P.; BOENDERS, J.; RAMSDEN, J. 1998. Human melanocytes and keratinocytes exposed to UVB or UVA in vivo show comparable levels of thymine dimers. Journal Investigation Dermatology 111: 936-940.