Effect of culture conditions on the enzymatic activity of Serratia marcescens against Tecia solanivora (Lepidoptera: Gelechiidae)
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The enzymatic activity of Serratia marcescens extracts was evaluated, previously inoculated or not on Tecia solanivora Povolny larvae, and successively grown in culture media supplemented or not with potential virulence inductors (wheat bran and homogenised T. solanivora larvae). The bacteria biomass obtained from these media were used to prepar enzymatic extracts for which the activity of the enzymes N-acetilglucosaminidase, quimoelastase protease PR-1 and ., Beta-esterase were determined. The bacteria were grown up in five successive cultures on these media, and in each culture these activities were also evaluated. We determined that successive growth of the bacteria on culture media supplemented with these potential substrates affected the extract enzymatic activities, increasing with the third successive growing of S. marcescens in the supplemented culture media, these activities being slighty higher when the bacteria grew on media supplemented with homogenised insect larvae.
BALAKRISHNAN, H.; SRINIVASAN, M.; RELE, M. 1997. Extracellular protease activities in relation to xylanase secretion in an alkalophilic Bacillus sp. Biotechnology Letters 19 (7): 599-601.
BISWAS, S.; MISHRA, A.; NANDA, G. 1988. Xylanase and B-xylosidase production by Aspergillus ochraceus during growth on lignocelluloses. Biotechnollogy Bioenginering 3 1: 613-616.
BOSA,C. 1996. Desarrollo de una metodología para evaluar la actividad insecticida de cepas nativas de Bacillus thuringiensis para el control de Spodoptera frugiperda J. E. Smith (Lepidoptera: Noctuidae). Trabajo de Grado de Biólogo. Universidad Distrital FJC, Bogotá. 98 p.
BRADFORD, M. 1976. Rapid sensitive method for the quantitation of microgram quantíties of protein utilizing the principie of protein dye binding. Analytical Biochemistry 72: 254-284
DECEDUE, C.: BROUSSARD,E.; LARSON.A.; BRAYMER,H. 1979. Purification and characterization of the extracellular proteinase of Serratia marcescens. Biochimica et Biophysica Acta 569: 293-301.
DUMUSOIS C,.PRIEST,F. 1993. Extracellular serine protease synthesis by mosquitopathogenic strains of Bacillus sphaericus. Journal of Applied Bacteriology 75: 416419.
FLACH, J.; PILET, P.; JOLLES, p. 1992. Wnats new in chitinase research? Experientia 48: 701-716.
FLYG, C.; XANTHOPOULOS, K. 1983. Insect pathogenic properties of S. marcescens. Passive and active resistance to insect immunity studied with protease-deficient and phage-resistant mutants. Journal of General Microbiology 129: 453-464.
GONZÁLES, M.; VALENCIA, A.; BUSTILLO, A. 1999. Incremento de la patogenicidad de B. bassiana hacia Hypothenemus hampei, utilizando cutícula de broca en el medio de cultivo. Resúmenes. XXVICongreso de la Sociedad Colombiana de Entomología. p. 156. Julio. Bogotá.
GRIMONT, P.; GRIMONT, F. 1978. The Genus Serratia. Annual Review Microbiology 32: 221-248.
MONREAL, J.; REESE, E. 1969. The chitinase of Serratia marcescens. Canadian Journal Microbiology 15: 689-696.
NÚÑEZ, V. 1993. Perspectivas de la biología molecular en el control de larvas de Scarabaeidae de importancia agrícola. En: Diversidad y manejo de plagas subterráneas. A, Morón (ed.). Sociedad Mexicana de Entomología e Instituto de Ecología, Xalapa, Veracruz, México. p 217-233.
SAMPSON, M.; GOODAY, G. 1998. Involvement of chitinases of Bacillus thuringiensis during pathogenesis in insects. Microbiology 144: 2189-2194.
SCHAERFFENBERG, B. 1964. Biological and environmental conditions for the development of mycoses caused by Beauveria sp., and Metarhizium sp. Journal of Invertebrate Pathology 6:8-20.
SOUZA, M.; ROBERTO, J.; MILAGRES, A. 1999. Solid-state fermentation for xylanase production by Thermoascus aurantiacus using response surface methodology. Applied Microbiologycal Biotechnology 52: 768-772.
ST.LEGER.R. 1997. Biology and Mechanisms of Insect-cuticle Invasion by Deuteromycete Fungal Pathogens. Cap LO. p. 21 1-225. En: Parasites and Pathogens of Insects. Vol 2. N. Beckage., S. Thompson.,y B. Federici (eds.) AcademicPress. San Diego, California.
ST. LEGER, R.; CHARNLEY, A.; COOPER, R. 1986a. Cuticle-Degrading Enzymes of Entomopathogenic Fungi: Cuticle Degradation in vitro by enzymes from entornopathogens. Journal of Invertebrate Pathology 47: 167-177.
ST. LEGER. R.; CHARNLEY, A.; COOPER, R. 1986b. Cuticle-degrading enzymes of entomopathogenic fungi: Mechanisms of interaction between pathogen enzymes and insect cuticle. Journal of Invertebrate Pathology 47: 295-302.
ST. LEGER, R.; CHARNLEY, A.; COOPER, R. 1986c. Cuticle-degrading enzymes of entomopathogenic fungi: synthesis in culture on cuticle. Journal of Invertebrate Pathology 48: 85-95.
THALER, J.; DUVIC, B.; GIVAUDANA, BOEMARE, N. 1998. Isolation and entomotoxic properties of the Xenorhabdus nematophilus Fl Lecithinase. Applied and Environmental Microbiology 64 (7): 2367-2373.
THONART, A.; COTES, A.; CAMPOS,D.; ROBLAIN, D.; LEPOIVRE,p. 1991. Modelling of uses of microorganisms in the degradation of natural polymers. En: Production and utilization of lignocellulosics. Galletti (ed.). Geambloux, Belgium. p. 79-89.
VILLAMIZAR, L. 1998. Efecto de la composición del medio de cultivo en la virulencia de sobre la langosta lIanera Rhammatocerus schistocercoides, Tesis de Maestría para optar al título de Microbióloga. UniversidadNacional de Colombia. Bogotá. I 12 p.
YOLJNG, M.; CARROAD, P. 1981. Dependance of extracellular chitinase activity of S. marcescens QMB1466on continuos culture dilution rateo Canadian Journal Microbiology 27: 142-144.
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