Effect of ultraviolet radiation on the insecticidal activity of a Spodoptera frugiperda (Lepidoptera: Noctuidae) nucleopolyhedrovirus
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The corn armyworm, Spodoptera frugiperda, is a polyphagous insect that causes important economic losses worldwide. Its control with highly toxic chemical insecticides is inefficient given the generation of resistance. Biological control with nucleopolyhedroviruses emerges as an alternative considering the efficacy and high specificity of these agents. However, baculoviruses are affected by environmental conditions, ultraviolet solar radiation being the most limiting factor for field efficacy. For this reason, the present work aimed to determine the susceptibility of a S. frugiperda nucleopolyhedrovirus to type UVA, UVB and UVC monochromatic ultraviolet radiation. Five concentrations of purified virus were exposed to each type of UV radiation over different times for a maximum of one hour. A negative effect of the three radiation types was observed on insecticidal activity, being greatest at higher exposure time and UV radiation energy. These results confirmed the need to develop a formulation which includes solar protectors for ensuring biopesticide efficacy under field conditions.
ALVES, S. 1986. Controle microbiano de insetos. Brasil, Ed. Manole. 406 p.
ASANO, S. 2005. Ultraviolet protection of a granulovirus product using ironoxide. Applied Entomology and Zoology 40 (2): 359-354.
BARRETO, M.; GUIMARAES, C.; TEIXEIRA, F.; PAIVA, E.; VALICENTE, F. 2005. Effect of Baculovirus spodoptera isolates in Spodoptera frugiperda larvae and their characterization by RAPD. Neotropical Entomology 34 (1): 67-75.
BATISTA, A.; ALVES, N.; AUGUSTO, N.; PEREIRA, R.; ALVES, E. 2001. Stability and persistence of two formulations containing Anticarsia gemmatalis nuclear polyhedrovirus (AgMNPV). Neotropical Entomology 30 (3): 411-416.
BRAGA, G.; FLINT, S.; MILLAR, C.; ANDERSON, A.; ROBERTS, D. 2001. Both solar UV A and UV B radiation impair conidial culturability and delay germination in the entomopathogenic fungus. Photochemistry and Photobiology 74 (5): 734-739.
BURGES, H. 1981. Microbial control of pest and plant diseases 1970-1980. Academic Press. London. 949 p.
DEVOTTO, L.; GERDING, M. 2003. Respuesta de dos aislamientos chilenos de Metarhizium anisopliae (Metschnikoff) Sorokin a la adición de un protector solar. Agricultura Técnica (Chile) 63 (4): 339-346.
EDGINGTON, S.; SEGURA, H.; DE LA ROSA, W.; WILLIAMS, T. 2000. Photoprotection of Beauveria bassiana: testing simple formulations for control of the coffee berry borer. International Journal of Pest Management 46 (3): 169-176.
FEDERICI, B. 1997. The baculoviruses. Plenum Press. New York. p. 33-56.
FERNÁNDEZ, J. L. 2003. Estimación de umbrales económicos para Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) en el cultivo del maíz. Universidad de Granma. Disponible en: http://www.inia.es/IASPV/2002/vol173/FERNANDEZ.pdf #search=’spodopterauniversidadfernandezumbral’.html. Fecha última revisión: 1 noviembre 2005.
FERRER, F. 2001. Biological Control of Agricultural Insect Pests in Venezuela; Advances, Achievements, and Future Perspectives. Biocontrol News and Information 22 (3): 67–74.
FINNEY, D. 1952. Probit analysis. Cambridge University Press. London. 151p.
GRIEGO, V.; MARTIGNONI, M.; CLAYCOMB, A. 1985. Inactivation of nuclear polyhedrosisnvirus (Baculovirus Subgroup A) by monochromatic radiation. Applied and Environmental Microbiology 49: (3) 709-710.
IGNOFFO, C.; BATZER, O. 1971. Microencapsulation and ultraviolet protectants to increase sunlight stability of insect virus. Journal of Economic Entomology 64 (4): 850-853.
IGNOFFO, C.; HOSTETTER, D.; SIKOROWSKI, P.; SUTTER, G.; BROOKS, W. 1977. Inactivation of representative species of entomopathogenic viruses, a bacterium, fungus and protozoan by an ultraviolet light source. Environmental Entomology 6 (3): 411-415.
IGNOFFO, C.; RICE, W.; McINTOSHI, A. 1989. Inactivation of nonoccluded baculovirus and baculovirus-DNA exposed to simulated sunlight. Environmental Entomology 18 (1): 177-183.
MARTIGNONI, M.; IWAI, P. 1985. Laboratory evaluation of new ultraviolet absorbers for protection of Douglas-fir tussock moth (Lepidotera: Lymantriidae) baculovirus. Journal of Economic Entomology 78 (4): 982-987.
MASCARENHAS, V.; LEONARD, B.; BURRIS, E.; GRAVES, J. B. 1996. Beet Armyworm (Lepidoptera: Noctuidae) Control on Cotton in Louisiana. Florida Entomologist 79 (3): 336.
MURILLO, R.; CABALLERO, P. 2006. El potencial de los baculovirus como agentes de control biológico de plagas. Phytoma España: La revista profesional de sanidad vegetal 179: 51-63.
NIEGEL, D. P.; DYLAN, G. J. 2003. Ecological roles of solar UV radiation: Towards and integrated approach (Review). Trends in Ecology and Evolution 18 (1): 48-54.
PAVONE, D. 2003. Formulación de un bioinsecticida con base en el hongo Nomuraea rileyi (Farlow) Samson para el control de Spodoptera frugiperda Smith (Lepidoptera: Noctuidae). Universidad Central de Venezuela Fondo Nacional de Ciencia, Tecnología e innovación de Venezuela. Disponible en: [en línea]: http://www.facyt.uc.edu.ve/biologia/webmstr/tesisdp.pdf. Fecha última revisión: 18 mayo 2007.
SAXENA, D.; BEN-DOV, E.; MANASHEROB, R.; BARAK, Z.; BOUSSIBA, S.; ZARITSKY, A. 2002. A UV tolerant mutant of Bacillus thuringiensis subsp. kurstaki producing melanin. Current Microbiology 44: 25-30.
SHAPIRO, M. 1989. Congo red as an ultraviolet protectant for the gypsy moth (Lepidoptera: Lymantriidae) nuclear polyhedrosis virus. Journal of Economical Entomology 82: (2) 548-550.
SMITS, N.; ROUGIER, M.; FARGUES, J.; GOUJET, R.; BONHOMME, R. 1996. Inactivation of Paecilomyces fumosoroseus conidia by diffuse and total solar radiation. FEMS Microbiology Ecology 21: 167-173.
TANADA, Y.; KAYA, H. 1993. Insect pathology. New York. Academic press. 623 p.
VALICENTE, F.; Da COSTA, E. 1995. Controle da lagarta do cartucho Spodoptera frugiperda (J. E. Smith), com o Baculovirus spodoptera, aplicado via água de irrigação. Anales de la Sociedade Entomologica do Brasil 24 (1): 61-67.
VALICENTE, F.; PEIXOTO, M.; PAIVA, E.; KITAJIMA, E. 1989.Identificação e purificação de um vírus da poliedrose nuclear da lagarta Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuide). Anales de la Sociedade Entomologica do Brasil 18: 71-81.
VILLAMIZAR, L.; ZEDDAM, J.; ESPINEL, C.; COTES, A. 2005. Implementación de técnicas de control de calidad en el proceso productivo de un bioplaguicida a base del granulovirus de Phthorimaea operculella PhopGV. Revista Colombiana de Entomología 31 (2): 127-132.
WAKEFIELD, G.; GREEN, M.; LIPSCOMB, S.; FLUTTER, B.2004. Modified titania nanomaterials for sunscreen applications-reducing free radical generation and DNA damage. Material Science and Technology 20 (8): 985-988.
WHO (World Health Organization). 1994. Ultraviolet Radiation. Environmental Health Criteria 14. Geneva. Switzerland. pp. 351.
ZAR, J. 1999. Biostatistical Analysis. Cuarta edición. Prentice Hall. New Jersey. 663 p.
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