Effect of cryopreservation on the viability and the biocontrol activity of Nomuraea rileyi against Spodoptera frugiperda (Lepidoptera: Noctuidae)
Main Article Content
The entomopathogenic fungus Nomuraea rileyi is one of the most promising alternatives for the control of Spodoptera frugiperda, the most important pest of maize in Colombia. In previous work, a native isolate of N. rileyi was selected that caused 100% mortality on larvae of the pest under laboratory conditions. However, biocontrol activity of microorganisms may be affected by different factors such as preservation method. This study determined the effect of cryopreservation in glycerol and peptone at - 70°C on its viability and biocontrol activity over time. From a suspension of 1 x 107 conidia.ml-1, germination percentage, number of colony-forming units (CFU) and percent efficacy of the microorganism on larvae were determined before cryopreservation (control), after 24 hours, 12 months and 18 months. An effect on the viability of the microorganism expressed as percent germination and as CFU was not evident 24 hours after cryopreservation with respect to the control; but after 12 months there was a delay of 18 hours to reach a percent germination above 90% and a one exponent loss to CFU. However, no significant differences were detected in moth mortality in any of the evaluated times, being higher than 96.4%. It is concluded that cryopreservation over 18 months does not affect the germination or the biocontrol activity but does affect the CFU.
BAHAMÓN, T.; AYCARDI, E.; OROZCO, J.; MARÍN, P.; BUSTILLO, A. 2001. Preservación de la patogenicidad de Beauveria bassiana (Bálsamo) Vuillemin (Moniliales: Moniliaceae) contra la broca del café en diferentes sistemas. Revista Colombiana de Biotecnología 3 (1): 80-90.
BOSA, C. F.; CHÁVEZ, D.; TORRES, L.; PARÍS, A.; VILLAMIZAR, L.; COTES, A. M. 2004. Evaluación de aislamientos nativos de Nomuraea rileyi para el control de Spodoptera frugiperda (Lepidoptera: Noctuidae). Revista Colombiana de Entomología 30 (1): 94-97.
BOUCIAS, D. G.; TIGANO, M. S.; SOSA GÓMEZ, D. R.; GLARE, T. R.; INGLIS, P. W. 2000. Genotypic properties of the entomopathogenic fungus Nomuraea rileyi. Biological Control (19): 124-38.
CASTILLO, L. F. 2003. Efecto de la composición del medio de cultivo en la virulencia de Nomuraea rileyi sobre el gusano cogollero del maíz Spodoptera frugiperda (Lepidoptera: Noctuidae). Trabajo de grado de Microbióloga Industrial. Pontificia Universidad Javeriana, Facultad de Microbiología Industrial. Bogotá.149 p.
CIBA – GEIGY. 1973. Cómo realizar un bioensayo. Manual de ensayos de campo. 215 p.
CLARKSON, J.; CHARNLEY, A. K. 1996. New insights into the mechanisms of fungal pathogenesis in insects. Trends in Microbiology 4 (5) 197-202.
CROAN, S. C.; BURDSALL, H. H.; RENTMEESTER, R. M.1999. Preservation of tropical wood-inhabiting basidiomycetes. Mycologia 91 (5): 908-916.
GARCÍA, J. L.; CLAVIJO, S. 1985. Incidencia del hongo Nomuraea sp (Deuteromycete: Ascomycotina) en larvas de Spodoptera frugiperda criadas en el laboratorio y alimentadas con plantas provenientes de campos comerciales de maíz. Boletín de Entomología Venezolana. N. S. 4(7): 53-59.
GARCÍA, F.; MOSQUERA, M.; VARGAS, C.; ROJAS, L. 2002. Control biológico, microbiológico y físico de Spodoptera frugiperda (Lepidotera: Noctuidae), plaga del maíz y otros cultivos en Colombia. Revista Colombiana de Entomología 28 (1): 53-60.
HALLSWORTH, J. E.; MAGAN, N. 1996. Culture age, temperature and pH affect the polyol and trehalose contents of fungal propagules. Applied and Environmental Microbiology 62 (7): 2435-2442.
HUBÁLEK, Z. 2003. Protectants used in the cryopreservation of microorganisms. Cryobiology 46 (3): 205-229.
LASTRA, C.C.; HAJEK, A. E.; HUMBER, R. A. 2002. Comparing methods of preservation for cultures of entomopathogenic fungi. Canadian Journal of Botany 80(10):1126-1130.
LEGARD, D. E.; CHANDLER, C. K. 2000. Cryopreservation of the strawberry pathogens Colletotrichum gloeosporoides, C. acutatum, C. fragariae and Phomopsis obscurans in a – 95°C mechanical ultralow temperature freezer. HortScience 35 (7): 1357-1361.
LEÓN, M.; PULIDO, J. L. 1991. Importancia del control natural del gusano cogollero Spodoptera frugiperda J. E. Smith. Memorias. Seminario Spodoptera frugiperda (El Gusano Cogollero) en sorgo, maíz y en otros cultivos. p. 78-82. Cali.
LEZAMA-GUTIÉRREZ, R.; JAMM, J.; MOLINA-OCHOA, J.; LÓPEZ-EDWARDS, M.; PESCADOR-RUBIO, A.; GONZÁLEZ-RAMÍREZ, M.; STYER, E. 2000. Occurence of entomophatogens of Spodoptera frugiperda (Lepidoptera: Noctuidae) in the mexican states of Michoacán, Colima, Jalisco and Tamaulipas. Armyworm Symposium. México. 8 p.
MINISTERIO DE AGRICULTURA Y DESARROLLO RURAL, 2004. Colombia: Producción, superficie y rendimiento de productos agropecuarios web site. http:// www.comunidadandina.org/estadisticas/ comp9099/agro-col.xls. Fecha último acceso:24 septiembre 2004.
MOTA, M.; CAMPOS, A.K.; ARAÚJO, J. V. 2003. Sporulation, radial growth and biomass production of A. robusta and M. thaumasium submitted to different methods of preservation. Brazilian Journal of Microbiology 34 (2): 157-160
RYAN, M.; JEFFRIES, P.; BRIDGE, P.; SMITH, D. 2001. Developing cryopreservation protocols to secure fungal gene function. CryoLetters (22) 125-134.
SIMIONE, F. 1998. Cryopreservation Manual. American Type Culture Collections – Nelge Nunc International Corp. Estados Unidos. 8 p.
SMITH, D.; THOMAS, V. E. 1997. Cryogenic light microscopy and the development of cooling protocols for the cryopreservation of filamentous fungi. World Journal of Microbiology and Biotechnology 14 (1): 49-57.
STOYCHEV, I.; HOMOLKA, L.; NERUD, F.; LISÁ, L. 1998. Activities of ligninolytic enzymes in some white-rot basidiomycete strains after recovering from cryopreservation in liquid nitrogen. International Journal of General and Molecular Microbiology 73 (3): 211-214.
VILLAMIZAR, L.; ARRIERO, C.; BOSA, F.; COTES, A. 2004. Desarrollo de preformulados a base de Nomuraea rileyi para el control del Spodoptera frugiperda (Lepidoptera: Noctuidae). Revista Colombiana de Entomología 30 (1): 99105.
- KARIM RAYMOND-C., FABIOLA ROJAS-B., EFRAÍN BENAVIDES-O., ALBA MARINA COTES, LAURA VILLAMIZAR, VANIA RONDEROS-J., PAOLA GARCÍA-M., Effect of entomopathogenic fungi on the cattle tick Boophilus microplus (Acari: Ixodida): use of pathogenicity activators , Revista Colombiana de Entomología: Vol. 30 No. 1 (2004)
- CARLOS ESPINEL, LISSETTE TORRES, ERIKA GRIJALBA, LAURA VILLAMIZAR, ALBA MARINA COTES, Preformulations for control of the whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) under laboratory conditions , Revista Colombiana de Entomología: Vol. 34 No. 1 (2008)
- CARLOS ESPINEL-CORREAL, EVERTH E. EBRATT, ALBA MARINA COTES, Evaluation of natives strains of Metarhizium anisopliae for biological control of llanera locust Rhammatocerus schistocercoides (Orthoptera:Acrididae) , Revista Colombiana de Entomología: Vol. 24 No. 1 (1998)
- CARLOS FELIPE BOSA-O., DORA CHÁVEZ, LISSETTE TORRES, ALEJANDRO PARÍS, LAURA VILLAMIZAR, ALBA MARINA COTES, Evaluation of native isolates of Nomuraea rileyi for the control of Spodoptera frugiperda (Lepidoptera: Noctuidae) , Revista Colombiana de Entomología: Vol. 30 No. 1 (2004)
- LAURA VILLAMIZAR, CAROLINA ARRIERO, CARLOS FELIPE BOSA-O., ALBA MARINA COTES, Development of preformulated products based on Nomuraea rileyi for control of Spodoptera frugiperda (Lepidoptera: Noctuidae) , Revista Colombiana de Entomología: Vol. 30 No. 1 (2004)
- CARLOS FELIPE BOSA, ANDREA CLAVIJO-MC, MIRIAM FRIDA KARLSSON, ALBA MARINA COTES, PETER WITZGALL, Response of Tecia solanivora (Lepidoptera: Gelechiidae) to volatile compounds of potato, Solanum tuberosum , Revista Colombiana de Entomología: Vol. 37 No. 1 (2011)
- OLGA YANETH PEREZ, ALEJANDRO RODRIGUEZ, ALBA MARINA COTES, A rapid and reliable method for the evaluation of Bacillus thuringiensis native strains against Tecia solanivora (Povolny) (Lepidoptera : Gelechiidae) , Revista Colombiana de Entomología: Vol. 23 No. 2 (1997)
- PAULA MARIÑO, LAURA VILLAMIZAR, CARLOS ESPINEV, ALBA MARINA COTES, Characterization of granular biopesticide prototypes based on Metarhizium anisopliae for the control ofAncognatha scarabaeoides (Coleoptera: Melolonthidae) , Revista Colombiana de Entomología: Vol. 30 No. 1 (2004)
- RICARDO PÉREZ, JAVIER GARCÍA-GONZÁLEZ, ALBA MARINA COTES, Effect of a biopesticide on the predatory activity of Delphastus pusillus (Coleoptera: Coccinellidae) , Revista Colombiana de Entomología: Vol. 34 No. 2 (2008)
- LAURA FERNANDA VILLAMIZAR-RIVERO, ALBA MARINA COTES, DANIEL URIBE, Relation between enzimatic activity and virulence of Metarhizium anisopliae over llanera locust (Orthoptera: Acrididae) , Revista Colombiana de Entomología: Vol. 27 No. 2 (2001)
Similar Articles
- María Camila Ortega, Sindy Lorena Mojica-Ramos, Erika Valentina Vergara-Navarro, Paola Sotelo-Cardona, Associated entomofauna with edible canna crops Canna indica (Cannaceae) in three areas of Colombia , Revista Colombiana de Entomología: Vol. 46 No. 1 (2020)
You may also start an advanced similarity search for this article.
Authors retain the copyright on their work and are responsible for the ideas expressed in them. Once a manuscript is approved for publication, authors are asked for a publication license for the term of legal protection, for all territories that allows the use, dissemination and disclosure of the same.