Control of the cattle tick Boophilus microplus with Metarhizium anisopliae, laboratory and field studies.
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The biocontrol potential of eight strains of Metarhizium anisopliae was evaluated for the cattle tick Boophilus microplus under laboratory and field conditions. The strain 137bm caused the highest effect on the reproductive capacity of the tick, decreasing tick oviposition by 90 and 96% when adult ticks were treated in vitro with 1x108 and 1x109 conidia/ ml. It also reduced egg viability by more than 98% in the same doses. In the field trials it was possible to reduce tick infestation by 75% in Holstein x Cebú cows when M. anisopliae 137bm was applied at a concentration of 1x108 conidias/ ml. Also, the fecundity of the ticks coming from cows treated with M. anisopliae was three times lower than that of the control group. On the other hand, strain 137bm applied to the meadow at a concentration of 5x1012 conidia/ha concentration caused a decrease in the larval tick population close to 86% one week after an application with conidia of M. anisopliae.
BARCELOS, A.; FIORIN, A.; MONTEIRO, A.; VERÍSSIMO, C.1998. Effects of Metarhizium anisopliae on the tick Boophilus microplus (Acari: Ixodidae) in stabled cattle. Journal of Invertebrate Pathology 71 (2): 189-191.
BENJAMÍN, M.; ZHIOUA, E.; OSTFELD, R. 2002. Laboratory and field evaluation of the entomopathogenic fungus Metarhizium anisopliae (Deuteromycetes) for controlling questing adult Ixodes scapularis (Acari: Ixodidae). Journal of Medical Entomology 39 (5): 723-728.
BITTENCOURT, V. 2000. Trials to control south american ticks with entomopathogenic fungi. Annals of the New York Academy of Sciences 916: 555-558.
BITTENCOURT, V.; MASSARD, C.; LIMA, A. 1994a. Ação do fungo Metarhizium anisopliae sobre a fase não parasitaria do ciclo biológico de Boophilus microplus. Revista Universidad Rural. Série Ciências da Vida 16 (1-2): 49-55.
BITTENCOURT, V.; MASSARD, C.; LIMA, A. 1994b. Ação do fungo Metarhizium anisopliae em ovos e larvo do garrapato Boophilus microplus. Revista Universidad Rural. Série Ciências da Vida 16 (1-2): 41-47.
BITTENCOURT, V.; SOUZA, E.; PERALVA, S.; MASCARENIAS, A.; ALVES, S. 1997. Avaliação da eficacia in vitro de dois isolados do fungo entomopatogênico Beauveria bassisana (Bals.) Vuill. Em fêmeas ingurgitadas de Boophilus microplus (Canestrini, 1887) (Acari: Ixodidae). Revista Brasileira de Parasitologia Veterinaria 6 (1): 49-52.
CHAGAS, A.; LEITE, R.; FURLONG, J.; PRATES, H.; PASSOS, W. 2003. Susceptibilidade do carrapato Boophilus microplus a solventes. Ciencia Rural 33 (1): 109-114.
DA COSTA, G.; SARQUIS, M.; DE MORAES, A.; BITTENCOURT, V. 2002. Isolation of Beauveria bassisana and Metarhizium anisopliae var anisopliae from Boophilus microplus tick (Canestrini, 1887); in Rio de Janeiro State; Brazil. Mycopathologia 154 (4): 207-209.
GINDIN, G.; SAMISH, M.; ALEKSEEV, E.; GLAZER, I. 2000. The susceptibility of Boophilus annulatus (Ixodidae) ticks to entomopathogenic fungi. Biocontrol Science and Technology 11 (1): 111-118.
GUEDES, A.; DA SILVA, I.; MASUDA, A.; SCHRANK, A.; HENNING, M. 2000. In vitro assessment of Metarhizium anisopliae isolates to control the cattle tick Boophilus microplus. Veterinary Parasitology 94 (1-2): 117-125.
HASSANAIN, M.; EL-GARBY, M.; ABDEL-GHAFFAR, F.; ELSHARABY, A.; ABDEL MEGEED, K. 1997. Biological control studies of soft and hard Ticks in Egypt. I The effect of Bacillus thurigiensis varieties of soft and hard tick (Ixodidae). Parasitology Research 83 (3): 209-213.
HILL, E. 1998. Entomopathogenic nematodes as control agents of developmental stags of the black legged tick: Ixodes scapularis. Journal of Parasitology 84(6): 1124-1127.
JONSSON, N.; MATSCHOSS, A.; PEPPER, P.; GREEN, P.; ALBRECHT, M.; HUNGERFORD, J.; ANSELL, J. 2000. Evaluation of TickGARDPLUS; a novel vaccine against Boophilus microplus; in lactating Holstein-Friesian cows. Veterinary Parasitology 88 (3-4): 275-285.
KAAYA, G. 2000. Laboratory and field evaluation for entomogenous fungi for tick control. Annals of the New York Academy of Sciences 916: 559-564.
KAAYA, G.; ASAN, S. 2000. Entomogenous fungi as promising biopesticides for tick control. Experimental and Applied Acarology 24 (12): 913-926.
KAAYA, G.; MWANGI, E.; OUNA, E. 1996. Prospects for biological control of livestock ticks; Rhipicephalus appendiculatus and Amblyomma variegatum; using the entomogenous fungi Beauveria bassiana and Metarhizium anisopliae. Journal of Invertebrate Pathology 67 (1): 15-20.
LÓPEZ, E.; ORDUZ, S. 2003. Metarhizium anisopliae and Trichoderma viride for control nests of the fungus-growing ant; Atta cephalotes. Biological Control 27 (2): 194-200.
LUZ, C.; FARGUES, J. 1997. Temperature and moisture requirements for conidial germination of an isolate of Beauveria bassiana; pathogenic to Rhodnius prolixus. Mycopathologia 138 (3): 117125.
PRATES, H.; LEITE BR.; CRAVEIRO CA.; OLIVEIRA D A. 1998. Identification of some chemical components of the essential oil from molasses grass (Melinis minutiflora Beauv.) and their activity against cattle-tick (Boophilus microplus). Journal of the Brazilian Chemical Society 9 (2): 193-197.
QUESADA-MORAGA, E.; SANTOS-QUIROS, R.; VALVERDEGARCIA, P.; SANTIAGO-ALVAREZ, C. 2004. Virulence; horizontal transmission; and subletal reproductive effects of Metarhizium anisopliae (Anamorphic fungi) on the German cockroach (Blattodea: Blattellidae). Journal of Invertebrate Pathology 87 (1): 51-58.
RODRÍGUEZ-VIVAS, R.; RODRIGUEZ-AREVALO F.; ALONSODIAZ M.; FRAGOSO-SANCHEZ H.; SANTAMARIA V.; ROSARIO-CRUZ R. 2006. Prevalence and potential risk factors for amitraz resistance in Boophilus microplus ticks in cattle farms in the State of Yucatan, Mexico. Preventive Veterinary Medicine 75 (3-4): 280-286.
RUIZ, L.; ORDUZ, S.; LÓPEZ, E.; GUZMAN, F.; PATARROYO, M.; ARMENGOL, G. 2007. Immune response in mice and cattle after immunization with a Boophilus microplus DNA vaccine containing bm86 gene. Veterinary Parasitology 144 (1-2): 138145.
SOUZA, E.; REIS, R.; BITTENCOURT, V. 1999. Evaluation of in vitro effect of the fungi Beauveria bassiana and Metarhizium anisopliae on eggs and larvae or Amblyomma cajennense. Revista Brasileira de Parasitologia Veterinaria 8 (2): 127-131.
ZHIOUA, E.; BROWING, M.; JOHNSON, P.; GINSBERG, H.; LEBRUN, R. 1997. Pathogenicity of the entomopathogenic fungus Metarhizium anisopliae (Deuterocycetes) to Ixodes scapularis (Acari: Ixodidae). Journal of Parasitology 83 (5): 815-818.
- ADRIANA ORTIZ, ALEJANDRO MADRIGAL, SERGIO ORDUZ, Behavior evaluation of the Atta cephalotes ants (Hymenoptera: Formicidae) during the contamination of the fungus garden with Trichoderma lignorum , Revista Colombiana de Entomología: Vol. 25 No. 2 (1999)
- FERNANDO VALLEJO, MIGUEL ANGEL MORÓN, SERGIO ORDUZ, First report and description of Phyllophaga obsoleta Blanchard (Coleoptera: Scarabaeoidea, Melolonthidae) in Colombia , Revista Colombiana de Entomología: Vol. 23 No. 1 (1997)
- NORA HELENA RESTREPO, MARÍA MERCEDES PATIÑO, SERGIO ORDUZ, THAIS DÍAZ, WILLIAM ROJAS, Characterization of genes coding for Bacillus thuringiensis ssp. medellin toxic proteins , Revista Colombiana de Entomología: Vol. 22 No. 1 (1996)
- THAIS DÍAZ, NORA RESTREPO, SERGIO ORDUZ, WILLIAM ROJAS, DISTRIBUTION AND ISOLATION OF Bacillus thuringiensis IN COLOMBIA , Revista Colombiana de Entomología: Vol. 19 No. 2 (1993)
- ELKIN LÓPEZ, MAGALLY ROMERO, ADRIANA ORTIZ, SERGIO ORDUZ, First report of Metarhizium anisopliae infecting queens of Atta cephalotes (Hymenoptera: Formicidae) from Colombia , Revista Colombiana de Entomología: Vol. 25 No. 1 (1999)
- NORA HELENA RESTREPO, MARÍA MERCEDES PATIÑO, SERGIO ORDUZ, Stability analysis of a recombinant bacterium with larvicidal activity , Revista Colombiana de Entomología: Vol. 22 No. 3 (1996)
- MAURICIO REALPE, SERGIO ORDUZ, Expression and Toxicity of Bacillus thuringiensis subesp. israelensis strains harbouring the gene encoding the main mosquitocidal 8-endotoxin from Bacillus thuringiensis subesp. medellin , Revista Colombiana de Entomología: Vol. 24 No. 2 (1998)
- CÉSAR SEGURA, FANNY GUZMÁN, MANUEL E. PATARROYO, SERGIO ORDUZ, Activation of the Bacillus thuringiensis subesp. medellin CryllBb protein by digestion with trypsin and Culex quinquefasciatus (Diptero: Culicidae) larval gut extracts , Revista Colombiana de Entomología: Vol. 24 No. 2 (1998)
- DIEGO VILLANUEVA, NUBIA VELÁSQUEZ, ESPERANZA RODRÍGUEZ, SERGIO ORDUZ, RAFAEL ARANGO, Molecular characterization of a Colombian Bacillus thuringiensis strain with activity against Tecia solanivora (Lepidoptera: Gelechiidae) , Revista Colombiana de Entomología: Vol. 35 No. 2 (2009)
- NORA RESTREPO, MARÍA M. PATIÑO, THAIS DIAZ, MARTHA C. TAMAYO, SERGIO ORDUZ, Biochemical, immunological and toxicological characteristics of the crystal proteins of Bacillus thuringiensis subsp. medellin , Revista Colombiana de Entomología: Vol. 22 No. 2 (1996)
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