DISTRIBUTION AND ISOLATION OF Bacillus thuringiensis IN COLOMBIA
Main Article Content
ln order to find natural enemies of mosquitoes, entomopathogenic bacteria were isolated from soil and dead mosquito larvae samples taken from different areas in the country. The methodology of Brownbridge and Margali for the isolation of spore-forming bacteria was used. The isolates showing toxicity towards Culex quienquefasciatus (Say) larvae were submitted to tests of biochemical identification and sensibility to antibiotics. Using larvae of C. quinquefasciatus, Aedes aegypti (L.) and Anopheles stephensi Liston the LC50 was determined. A total of 79 isolates of Bacillus thuringiensis Berliner toxic to mosquito larvae were found. The isolates CIB 163-131 and CIB 24-273 were found to belong to the serotype H-30,.new for the world, and it was nominated Bacillus thuringiensis subsp. medellin (B.t.m.). The toxic proteins of those isolates showed immunological characteristics different from those of B. thuringiensis subsp. israelensis, which is well distributed in the tropical warm areas of Colombia.
- ELKIN LÓPEZ, GUSTAVO LÓPEZ, SERGIO ORDUZ, Control of the cattle tick Boophilus microplus with Metarhizium anisopliae, laboratory and field studies. , Revista Colombiana de Entomología: Vol. 35 No. 1 (2009)
- ADRIANA ORTIZ, ALEJANDRO MADRIGAL, SERGIO ORDUZ, Behavior evaluation of the Atta cephalotes ants (Hymenoptera: Formicidae) during the contamination of the fungus garden with Trichoderma lignorum , Revista Colombiana de Entomología: Vol. 25 No. 2 (1999)
- FERNANDO VALLEJO, MIGUEL ANGEL MORÓN, SERGIO ORDUZ, First report and description of Phyllophaga obsoleta Blanchard (Coleoptera: Scarabaeoidea, Melolonthidae) in Colombia , Revista Colombiana de Entomología: Vol. 23 No. 1 (1997)
- NORA HELENA RESTREPO, MARÍA MERCEDES PATIÑO, SERGIO ORDUZ, THAIS DÍAZ, WILLIAM ROJAS, Characterization of genes coding for Bacillus thuringiensis ssp. medellin toxic proteins , Revista Colombiana de Entomología: Vol. 22 No. 1 (1996)
- ELKIN LÓPEZ, MAGALLY ROMERO, ADRIANA ORTIZ, SERGIO ORDUZ, First report of Metarhizium anisopliae infecting queens of Atta cephalotes (Hymenoptera: Formicidae) from Colombia , Revista Colombiana de Entomología: Vol. 25 No. 1 (1999)
- NORA HELENA RESTREPO, MARÍA MERCEDES PATIÑO, SERGIO ORDUZ, Stability analysis of a recombinant bacterium with larvicidal activity , Revista Colombiana de Entomología: Vol. 22 No. 3 (1996)
- MAURICIO REALPE, SERGIO ORDUZ, Expression and Toxicity of Bacillus thuringiensis subesp. israelensis strains harbouring the gene encoding the main mosquitocidal 8-endotoxin from Bacillus thuringiensis subesp. medellin , Revista Colombiana de Entomología: Vol. 24 No. 2 (1998)
- CÉSAR SEGURA, FANNY GUZMÁN, MANUEL E. PATARROYO, SERGIO ORDUZ, Activation of the Bacillus thuringiensis subesp. medellin CryllBb protein by digestion with trypsin and Culex quinquefasciatus (Diptero: Culicidae) larval gut extracts , Revista Colombiana de Entomología: Vol. 24 No. 2 (1998)
- DIEGO VILLANUEVA, NUBIA VELÁSQUEZ, ESPERANZA RODRÍGUEZ, SERGIO ORDUZ, RAFAEL ARANGO, Molecular characterization of a Colombian Bacillus thuringiensis strain with activity against Tecia solanivora (Lepidoptera: Gelechiidae) , Revista Colombiana de Entomología: Vol. 35 No. 2 (2009)
- NORA RESTREPO, MARÍA M. PATIÑO, THAIS DIAZ, MARTHA C. TAMAYO, SERGIO ORDUZ, Biochemical, immunological and toxicological characteristics of the crystal proteins of Bacillus thuringiensis subsp. medellin , Revista Colombiana de Entomología: Vol. 22 No. 2 (1996)
Authors retain the copyright on their work and are responsible for the ideas expressed in them. Once a manuscript is approved for publication, authors are asked for a publication license for the term of legal protection, for all territories that allows the use, dissemination and disclosure of the same.