Stability analysis of a recombinant bacterium with larvicidal activity
Main Article Content
The recombinant DNA technology offers new alternatives in the production of improved microorganisms for large scale production. However, the stability of recombinant plasmids during long culture periods is a serious problem for industrial use. The aim of this work was to introduce in the native aquatic bacterium Bacillus thuringiensis subsp. israeliensis CIB 30-0501b the plasmid pGSP11 that contains the genes coding the binary toxin of B. sphaericus and to analyze the expression and stability of this plasmid under laboratory conditions. This analysis showed that the bacterium grew best in the culture medium Lucia Bertani broth at 28°C with tetracicline (30 mg/mi). The presence of the pGSP11 plasmid was detected after eight hours of growth, coinciding with the moment that the expression of the plasmid-coded toxins started. An increase in the culture temperature was always correlated with a decrease in toxin synthesis which was not accompanied by plasmid loss. The presence of the selection antibiotic showed an important effect on plasmid conservation.
- ELKIN LÓPEZ, GUSTAVO LÓPEZ, SERGIO ORDUZ, Control of the cattle tick Boophilus microplus with Metarhizium anisopliae, laboratory and field studies. , Revista Colombiana de Entomología: Vol. 35 No. 1 (2009)
- ADRIANA ORTIZ, ALEJANDRO MADRIGAL, SERGIO ORDUZ, Behavior evaluation of the Atta cephalotes ants (Hymenoptera: Formicidae) during the contamination of the fungus garden with Trichoderma lignorum , Revista Colombiana de Entomología: Vol. 25 No. 2 (1999)
- ELKIN LÓPEZ, MAGALLY ROMERO, ADRIANA ORTIZ, SERGIO ORDUZ, First report of Metarhizium anisopliae infecting queens of Atta cephalotes (Hymenoptera: Formicidae) from Colombia , Revista Colombiana de Entomología: Vol. 25 No. 1 (1999)
- FERNANDO VALLEJO, MIGUEL ANGEL MORÓN, SERGIO ORDUZ, First report and description of Phyllophaga obsoleta Blanchard (Coleoptera: Scarabaeoidea, Melolonthidae) in Colombia , Revista Colombiana de Entomología: Vol. 23 No. 1 (1997)
- THAIS DÍAZ, NORA RESTREPO, SERGIO ORDUZ, WILLIAM ROJAS, DISTRIBUTION AND ISOLATION OF Bacillus thuringiensis IN COLOMBIA , Revista Colombiana de Entomología: Vol. 19 No. 2 (1993)
- DIEGO VILLANUEVA, NUBIA VELÁSQUEZ, ESPERANZA RODRÍGUEZ, SERGIO ORDUZ, RAFAEL ARANGO, Molecular characterization of a Colombian Bacillus thuringiensis strain with activity against Tecia solanivora (Lepidoptera: Gelechiidae) , Revista Colombiana de Entomología: Vol. 35 No. 2 (2009)
- MAURICIO REALPE, SERGIO ORDUZ, Expression and Toxicity of Bacillus thuringiensis subesp. israelensis strains harbouring the gene encoding the main mosquitocidal 8-endotoxin from Bacillus thuringiensis subesp. medellin , Revista Colombiana de Entomología: Vol. 24 No. 2 (1998)
- CÉSAR SEGURA, FANNY GUZMÁN, MANUEL E. PATARROYO, SERGIO ORDUZ, Activation of the Bacillus thuringiensis subesp. medellin CryllBb protein by digestion with trypsin and Culex quinquefasciatus (Diptero: Culicidae) larval gut extracts , Revista Colombiana de Entomología: Vol. 24 No. 2 (1998)
- NORA HELENA RESTREPO, MARÍA MERCEDES PATIÑO, SERGIO ORDUZ, THAIS DÍAZ, WILLIAM ROJAS, Characterization of genes coding for Bacillus thuringiensis ssp. medellin toxic proteins , Revista Colombiana de Entomología: Vol. 22 No. 1 (1996)
- NORA RESTREPO, DIANA GUTIÉRREZ, MARÍA MERCEDES PATIÑO, ISABELLE THIÉRY, ARMELLE DELÉCLUSE, SERGIO ORDUZ, Cloning, expression and toxicity of a mosquitocidal toxin gene of Bacillus thuringiensis subsp. medellin , Revista Colombiana de Entomología: Vol. 23 No. 2 (1997)
Authors retain the copyright on their work and are responsible for the ideas expressed in them. Once a manuscript is approved for publication, authors are asked for a publication license for the term of legal protection, for all territories that allows the use, dissemination and disclosure of the same.