Fine structure of Beauveria bassiana chlamydospores in culture amended with cooper oxychloride
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The entomogenous fungos, Beauveria bassiana, pathogen of the coffee berry borer, Hypothenemus hampei, when growing on SDA or rice rarely produces chlamydospore; however, when SDA and rice were amended with 600, 3000 & 6000 ppm of copper oxychloride, ninety-nine isolates from different insect orders produced a large number of chlamydospores. The chlamydospore cytoplasm of all isolates contain large lipid deposits and vacuoles, mitochondria, and endoplasmic reticulum, all typical of fungal cells. The induction of chlamydospores in B. bassiana may be of interest in developing formulations for the control of terrestrial insects due to the persistence of this structure. This is the first time that a chlamydospore formation in B. bassiana is reported.
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