Please ensure Javascript is enabled for purposes of website accessibility
Skip to main content Skip to main navigation menu Skip to site footer
10.25100/socolen.v24i1.9827
Submitted: 26-05-2020
Published: 15-01-1998

First report of the B biotype of Bemisia tabaci (Homoptera: Aleyrodidae) in Colombia

Centro Internacional de Agricultura Tropical (CIAT)
Centro Internacional de Agricultura Tropical (CIAT)
Centro Internacional de Agricultura Tropical (CIAT)
Corporación Colombiana de Investigación Agropecuaria (CORPOICA)
Keywords: Bemisia tabaci, Non-specific esterases, RAPD, Hosts, Symptoms, Silverleaf

How to Cite

1.
QUINTERO C, CARDONA C, RAMÍREZ D, JIMÉNEZ N. First report of the B biotype of Bemisia tabaci (Homoptera: Aleyrodidae) in Colombia. Rev. Colomb. Entomol. [Internet]. 1998 Jan. 15 [cited 2026 Oct. 24];24(1):23-8. Available from: https://revistacolombianaentomologia.univalle.edu.co/index.php/SOCOLEN/article/view/9827
Metrics

327 | 289




 

Download:

Abstract

The presence of the Bemisia tabaci (Gennadius) B biotype was confirmed in Colombia by means of biochemical, molecular, and etiological tests. The biochemical identification consisted of the determination of non-specific esterase banding patterns. Polymorphism among samples collected in Córdoba, Sucre, Atlántico, Valle del Cauca, and Cundi­namarca was found, and almost all of the samples were identified as the B biotype, by comparison with A biotype samples drawn from a colony maintained at CIAT for several years. A high degree of polymorphism was found in the B biotype, which was widespread in the area, attacking 23 species of crop plants and 11 weeds. The similarity between a/B esterase patterns of the two biotypes was only 20%. At the molecular level, RAPD amplification products obtained with the primers OPA-04, OPA-11 and OPA-17 were also found to be different between biotypes A and B. Furthermore, after feeding the two biotypes en four cucurbit species, the silver leaf symptom was induced only on Cucurbita moschata (Duchesne) by B biotype nymphs, 21 days after the infestation. The B biotype was found to be more aggressive since it successfully colonized C. mixta Pang., C. pepo L., C. ficifolia Bouché y C. moschata, 23 to 33% faster than biotype A.

326 288
  1. BLACK, W.C.; DUTEAU, N.M.; PUTERKA, G.J.; NECHOLS, J.R.; PETTORINI, J.M. 1992. Use of random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) to detect DNA polymorphisms in aphids. Bulletin of Entomological Research (Reino Unido) 82:151-159.
  2. BROWN, J.K. 1993. Evaluación crítica sobre los biotipos de mosca blanca en América, de 1989 a 1992. pp. 1-9. En: Memorias del Taller Cen­troamericano y del Caribe sobre moscas blancas. CATIE, Turrialba, Costa Rica. Agosto 3­5, 1992.
  3. BROWN, J.K.; FROHLICH, D.R.; ROSELL, R.C. 1995a. The sweetpotato or silverleaf whitefly: biotypes of Bemisia tabaci or a species complex? Annual Review of Entomology (Es­tados Unidos) 40, p. 511-534
  4. BROWN, J.K.; COATS, S.A.; BEDFORD, I.D.; MARKHAM, P.G.; BIRD, J.; FROHLICH, D.R. 1995b. Characterization and distribution of esterase electromorphs in the whitefly, Bemisia tabaci (Genn.) (Homoptera; Aleyrodidae). Biochemical Genetics (Estados Unidos) 33, p. 205- 214.
  5. COSTA, H.S.; BROWN, J.K. 1991. Variation and biological characteristics and esterase patterns among populations of Bemisia tabaci Genn. and the association of one population with silverleaf symptom development. Entomologia Experimen­talis et Applicata (Holanda) 61:211-221.
  6. GAWEL, N.J.; BARTLETT, A.C. 1993. Characte­rization of differences between whiteflies using RAPD-PCR. Insect Molecular Biology (Esta­dos Unidos) 2: 33-38.
  7. PERRING, T.M.; FARRAR, C.A.; BELLOWS, T.S. ; COOPER, A.D.; RODRIGUEZ, R.J. 1993. Evidence for a new species of whitefly: UCR findings and implications. California Agriculture (Estados Unidos) 47: 7-8.
  8. PERRING, T.M. 1996. Biological differences of two species of Bemisia that contribute to adaptative advange. pp. 3-16. In: Bemisia: 1995 Taxonomy, Biology, Damage, Control and Management. (Gerling D & RT Mayer, eds.). Intercept Limited, Andover, UK.
  9. PRABHAKER, N.; COUDRIET, D.L.; MEYER­DIRK, D.E. 1987. Discrimination of three whitefly species (Homoptera; Aleyrodidae) by electrophoresis of non-specific esterases. Journal of Applied Entomology (Alemania) 103:447-451.
  10. ROHLF, F.J. 1994. NTSYS-pc. Numerical Taxonomy and Multivariate Analysis System, version 1.80. Exeter Software. Applied Biostatistics, Inc.
  11. SAMBROOK, J.; FRITSCH, E.F.; MANIATIS, T. 1989. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, New York.
  12. WOOL, D.; GERLING, D.; NOLT, B.L.; CONSTANTINO, L.M.; BELLOTTI, A.C.; MORALES, F.J. 1989. The use of electropho­resis for identification of adult whiteflies (Homoptera, Aleyrodidae) in Israel and Colom­bia. Journal of Applied Entomology (Alema­nia) 107:344-350.
Download data is not yet available.

How to Cite

1.
QUINTERO C, CARDONA C, RAMÍREZ D, JIMÉNEZ N. First report of the B biotype of Bemisia tabaci (Homoptera: Aleyrodidae) in Colombia. Rev. Colomb. Entomol. [Internet]. 1998 Jan. 15 [cited 2026 Oct. 24];24(1):23-8. Available from: https://revistacolombianaentomologia.univalle.edu.co/index.php/SOCOLEN/article/view/9827
Citations
Crossref
Scopus
Google Scholar
Europe PMC

Authors retain the copyright on their work and are responsible for the ideas expressed in them. Once a manuscript is approved for publication, authors are asked for a publication license for the term of legal protection, for all territories that allows the use, dissemination and disclosure of the same.