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In order to select entomopathogenic isolates against Premnotrypes vorax four isolates of Beauveria bassiana, three of Beauveria brongniartii and one of Metarhizium anisopliae, were evalua­ted under laboratory conditions. Adults of Premnotrypes vorax were treated with a unique concentration containing 1x108 propagules/ml, of each of them, and mortality was evaluated daily. To deter­mine their lethal concentrations 50 (CL5o) and 90 (CL90) successive concentrations of the selected isolates were used, from 1x105 to 1x109 propagules/ml. Biocontrol activity by a combination of selected isolates of Metarhizium anisopliae and Beauveria bassiana was determined; the bioassay was performed by using the CL50 oí each of them, and comparing the result with the mortality obtained by using twiceCLSI~of individual isolates. From the eight isolates evaluated Mt.L1 of Metarhizium anisopliae and Bv.L2 of Beauveria bassiana were selected because caused 100% insects mortality after 24 and 25 days respectively. These isolates showed lethal concentrations 50 y 90 of 13x105 and 50x106 propagules/ml, respectively for Mt.L1 and 55x105 and 48x107 propagules/ml, respectively for Bv.L2. The mortality observed in Premnotrypes vorax was significantly superior when the isolates were utilized in combination because produced 100% of mortality ten days after the application, while when the individual activity was evaluated with the isolate Mt.L1 53,33% the mortality was observed and with the isolate Bv.L2 the mortality was of the 43,44%, in the same time.

TORRES-TORRES, L., & COTES, A. M. (1999). Biocontrolator activity of entomogenous fungí against Premnotrypes vorax (Coleoptera: Curculionidae) throught their utilization individual or combined. Revista Colombiana De Entomología, 25(2), 121–129. https://doi.org/10.25100/socolen.v25i2.9761
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