Main Article Content


We evaluated the yield of a potassium acetate protocol with minor modifications for the extraction of genomic DNA. The results showed that using the modified protocol gave a higher DNA yield (0.61, and concentration 37.34 ng/µL) was obtained with respect to the original protocol. The amplification of DNA by PCR for mitochondrial regions demonstrated the high quality of this DNA for molecular population studies, in less time and cost, without the use of highly toxic reagents.

GOLCZER, G., & ARRIVILLAGA, J. (2008). Modification of a standard protocol for DNA extraction from smaller sandflies (Phlebotominae: Lutzomyia). Revista Colombiana De Entomología, 34(2), 199–202.

ARANSAY, A. M.; SCOULICA, E.; CHANIOTIS, B.; TSELENTIS, Y. 1999. Typing of sandflies from Greece and Cyprus by DNA polymorphism of 18S rRNA gene. Insect Molecular Biology 8: 179-184.

DE ARMAS, Y.; RODRÍGUEZ, M. M.; María M.; BISSET., J.A. 2005. Modificación de un método de extracción de ADN genómico de Aedes aegypti (Diptera: Culicidae). Revista Colombiana de Entomología 31: 203-6.


The taxonomic status of genetically divergent populations of Lutzomyia longipalpis (Diptera: Psychodidae) based on the distribution of mitochondrial and isozyme Variation. Journal of Medical Entomology 40: 615-627.

BEATI, L.; CÁCERES, A. G.; LEE, J. A.; MUNSTERMANN, L. E. 2004. Systematic relationships among Lutzomyia sand flies (Diptera: Psychodidae) of Perú and Colombia based on the analysis of 12S and 28S ribosomal DNA sequences. Journal of Parasitology 34: 225-34.

BENDER, W.; SPIERER, P.; HOGNESS, D. S. 1983. Chromosomal walking and jumping to isolate DNA from the ace and rosy loci and the Bithorax complex in Drosophila melanogaster. Journal of Molecular Biology 168: 17-33.

CABRERA, O. L.; MUNSTERMANN, L. E.; CÁRDENAS, R.; FERRO, C. 2003. PCR as a tool in confirming the experimental transmission of Leishmania chagasi to hamsters by Lutzomyia longipalpis (Diptera: Psychodidae). Biomedica 23: 239-244

COLLINS, F. H.; MÉNDEZ, M. A.; RASMUSSEN, M. O.; MEHAFFEY, P. C.; BESANSKY, N. J.; FINNERTY, V. 1987. A ribosomal RNA gene probe differentiates member species of the Anopheles gambiae complex. Americam. Journal of Tropical Medicine and Hygiene 37: 37-41.

ESSEGHIR, S.; READY, P. D.; KILLICK-KENDRICK, R.; BENISMAIL, R. 1997. Mitochondrial haplotypes and phylogeography of Phlebotomus vectors of Leishmania major. Insect Molecular Biology 6: 211-25.

HODGKINSON, V. H.; BIRUNGI, J.; QUINTANA, M., DIETZE, R.; MUNSTERMANN, L. E. 2003. Mitochondrial cytochrome b variation in populations of the visceral leishmaniasis vector Lutzomyia longipalpis across eastern Brazil. American Journal of Tropical Medicine and Hygiene 69: 386-92.

ISH-HOROWIZ, D. 1982. Personal communication in: rate of turnover of structural variants in the rDNA gene family of Drosophila melanogaster. Nature 295: 564-568.

JORQUERA, A.; GONZÁLEZ, R.; MARCHÁN-MARCANO, E.; OVIEDO, M.; MATOS, M. 2005. Multiplex-PCR for detection of natural Leishmania infection in Lutzomyia spp. captured in an endemic region for cutaneous leishmaniasis in state of Sucre, Venezuela. Memories Instituto Oswaldo Cruz 100: 43-46.

KATO, H.; UEZATO, H.; KATAKURA, K.; CALVOPIN, M.; MARCO, J. D.; BARROSO, P. A.; GÓMEZ, E. A.; MIMORI, T.; KORENAGA, M. T.; IWATA, H.; NONAKA, S.; YOSHIHISA, A. 2005. Detection and identification of Leishmania species within species naturally infected sandflies in the andean areas of Ecuador by polymerase chain reaction. American Journal Tropical. Medical and Hygiene 72: 87-93.

MICHALSKY, E. K.; CONSUELO, L.; FORTES-DIAS, P.; PIMENTA, F. P.; NAGILA, F. C.; SECUNDINO, E. S. DIAS. 2002. Assessment of PCR in the detection of Leishmania spp. in experimental infected individual phlebotominae sandflies (Diptera: Psychodidae): Revista Instituto de Medical Tropical Sao Paulo 44: 255-259.

NODARSE, J. F.; RODRÍGUEZ, J.; FUENTES, O.; CASTEX, M.; FERNÁNDEZ-CALIENES, A. 2004. Comparación entre 5 métodos para la extracción de ADN de triatomínos, su utilización en la técnica de ADN polimórfico amplificado al azar. Revista Cubana de Medicina Tropical 56: 208-213.

PARVISI, P.; AMIRKHAMI, A. 2008. Mitochondrial DNA characaterization of Sergentomyia sintoni populations and fiding mammalian leishmania infections in this sandflies using ITSrDNA. Iranian Journal of Veterinary 9: 10-18.

READY, P. D.; LAINSON, R.; SHAW, J. J.; SOUZA, A. A. 1991.DNA probe for distinguishing Psychodopygus wellcomi from Psychodopygus complexus (Diptera: Psychodidae). Memories Instituto Oswaldo Cruz 86: 41-49.

SOUZA, L.; FALQUETO, A.; BIRAL DOS SANTOS, C.; GRIMALDI, G.; CUPOLILLO, E. 2007. Genetic structure of Lutzomyia (Nyssomyia) intermedia populations from to ecologic regions in Brazil where transmission of Leishmania (Vianni) brasiliensis reflects distinct eco-epidemiologic features. American Journal of Tropical Medicine and Hygiene 76: 559-565.

TESTA, J. M.; MONTOYA-LERMA, J.; CADENA, H.; OVIEDO, M.; READY, P. D. 2002. Molecular identification of vectors of Leishmania in Colombia: Mitochondrial introgression in the Lutzomyia townsendi series. Acta Tropica 84: 205-18.

TORGERSON, D. G.; LAMPO, M.; VELÁZQUEZ, Y.; WOO, P. T. 2003. Genetic relationships among some species groups within the genus Lutzomyia (Diptera: Psychodidae). American Journal of Tropical Medicine and Hygiene 69: 484-93.

VIVERO, R. J.; CONTRERAS-GUTIÉRREZ, BEJARANO, M. A. E; Elías, E. 2007. Analysis of the primary and secondary structure of the mitochondrial serine transfer RNA in seven species of Lutzomyia. Biomedica 27: 429-438.

YOUNG, D. G.; DUNCAN, M. A. 1994. Guide to the identification and geographic distribution of Lutzomyia sand flies in Mexico, the West Indies, central and South America (Diptera: Psychodidae). Associated Publishers American Entomological Institute. Gainsville, Florida. 881 p.


Download data is not yet available.