Identification of Spodoptera frugiperda biotypes (Lepidoptera: Noctuidae) through using mitochondrial and nuclear markers

https://doi.org/10.25100/socolen.v34i2.9270
COI region PCR-RFLP´s Msp1 restriction enzyme FR region Hybrids

Article Sidebar

PDF (Español)

Published: Jul 15, 2008
Issue: Vol. 34 No. 2 (2008)
Section Agriculture / Research paper

Publcation metrics

82




 


Main Article Content

Authors

ANA MARÍA VÉLEZ-ARANGO Universidad Nacional de Colombia, Sede Medellín
RAFAEL EDUARDO ARANGO-I. Corporación para Investigaciones Biológicas (CIB)
DIEGO VILLANUEVA M. Corporación para Investigaciones Biológicas (CIB)
ELIZABETH AGUILERA-G. Corporación Colombiana de Investigación Agropecuaria (CORPOICA)
CLARA INÉS SALDAMANDO-B. Universidad Nacional de Colombia, Sede Medellín
Abstract

Spodoptera frugiperda is considered a primary pest in corn crops and a secondary pest in rice, cotton, sorghum and pastures. It exhibits two biotypes (corn and rice) that are almost identical morphologically, but differ in their genetic composition and resistance to insecticides and Bacillus thuringiensis, with the corn biotype more resistant than the rice biotype. This work is the first molecular identification in Colombia of these biotypes for larvae collected from corn, rice and cotton crops in the department of Tolima by using a PCR-RFLP of the mitochondrial gene cytochrome oxidase subunit I (COI) for the identification of corn biotype, and a PCR amplifying a FR tandem region of the nuclear DNA to identify the rice biotype. The results differentiated three populations of S. frugiperda: a) corn biotype, composed of individuals that generated digestion products with the enzyme MspI of the COI gene, b) rice biotype, composed of individuals that generated amplification products higher than 500 bp with the FR marker, and finally c) a group of individuals classified as “hybrids” between these markers, product of the cross between a corn biotype female and rice biotype male and vice versa. Individuals genotyped as the corn biotype were collected exclusively from corn and cotton crops and the individuals genotyped as the rice biotype were mainly collected from rice crops and in low proportions from corn and cotton crops.

How to Cite
VÉLEZ-ARANGO, A. M., ARANGO-I., R. E., VILLANUEVA M., D., AGUILERA-G., E., & SALDAMANDO-B., C. I. (2008). Identification of Spodoptera frugiperda biotypes (Lepidoptera: Noctuidae) through using mitochondrial and nuclear markers. Revista Colombiana De Entomología, 34(2), 145–150. https://doi.org/10.25100/socolen.v34i2.9270
References

ADAMCZYK, J. R.; H OLLOWAY, J. J.; LEONARD, J. W.; GRAVES, J. B. 1997. Susceptibility of fall armyworm collected from different plant hosts to selected insecticides and transgenic Bt cotton. Journal of Cotton Science 1 (1): 21-28.

ÁLVAREZ, J. A.; SÁNCHEZ, G. 1983. Variación en el número de instares de Spodoptera frugiperda (J. E. Smith). Revista Colombiana de Entomología 9 (1): 43-49.

BUSATO, G. R.; GRUZTMACHER, A. D.; DE OLIVEIRA, A. C.; VIEIRA, E. A.; ZIMMER, P. A.; KOPP, M. M.; DE BANDEIRA, J.; MAGALAHES, T. 2004. Analise da estrutura e diversidade molecular de poblacioes de Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) asociadas as culturas de milho e arroz no Rio Grande do Sul. Neotropical Entomology 33: 709-716.

BUSTILLO, A. E.; POSADA, F. J. 1983. Patogenicidad de un aislamiento de Nomuraea rileyi sobre larvas del cogollero del maíz Spodoptera frugiperda. Revista Colombiana de Entomología 12 (1): 5-15.

CLARK, P. L.; MOLINA-OCHOA, J.; MARTINELLI, S.; SKODA, S. R..; ISENHOUR, D. I.; LEE, D. J.; KRUMM, J., T.; FOSTER, J. E. 2007. Population variation of the fall armyworm, Spodoptera frugiperda, in western hemisphere. Journal of Insect Science 7: 05.

DRES, M.; MALLET, J. 2002. Host races in plant-feeding insects and their importance sympatric speciation. Philosophical Transactions of the Royal Society of Science 357: 471-492.

GARCÍA, F.; MOSQUERA, M. T.; VARGAS, C.; ROJAS, L. A 2002. Control biológico, microbiológico y físico de Spodoptera frugiperda (Lepidoptera: Noctuidae) plaga del maíz y otros cultivos en Colombia. Revista Colombiana de Entomología 28 (1): 53-60.

GENSTAT. 2003. GENSTAT 5.0 Release 4.23DE, Lawes Agricultural Trust, Rothamsted Experimental Station.

LEVY, C. H.; GARCÍA-MARUNIAK, A.; MARUNIAK, J. 2002. Strain identification of Spodoptera frugiperda (Lepidoptera: Noctuidae) insects and cell line: PCR-RFLP of cytocrome oxidase c subunit I gene. Florida Entomologist 85 (1): 186-190.

LÓPEZ-EDWARDS, M.; HERNÁNDEZ-MENDOZA, J. L.; PESCADOR-RUBIO, A.; MOLINA-OCHOA, J.; LEZMAGUTIERREZ, R.; HAMM, J. J.; WISEMAN, B. R. 1999. Biological differences between five populations of fall armyworm (Lepidoptera: Noctuidae) collected from corn in Mexico. Florida Entomologist 82 (2): 254-262.

LU, Y. J.; KOCHERT, G. D.; ISENHOUR, D. J.; ADANG, M. J. 1994. Molecular characterization of a strain-specific repeated DNA sequence in the fall armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae). Insect Molecular Biology 3: 123–130.

MARTINELLI, S.; CLARK, P. L.; ZUCCHI, M.I.; SILVAFILHO, M.C.; FOSTER, J. E. OMOTO, C. 2007. Genetic structure and molecular variability of Spodoptera frugiperda (Lepidoptera: Noctuidae) collected in maize and cotton field in Brazil. Bulletin of Entomological Research 97: 225-231.

MCMICHAEL, M.; PASHLEY, D. P. 1999. Differences in amplified fragment-length polymorphism in fall armyworm (Lepidoptera: Noctuidae) host strains. Annals of the Entomological Society of America 92 (2): 175-181.

MEAGHER, R. L.; GALLO-MEAGHER M. 2003. Identifying host strains of fall armyworm (Lepidoptera: Noctuidae) in Florida using mitochondrial markers. Florida Entomologist 86 (4): 450455.

NAGOSHI, R. D.; MEAGHER, R. L. 2003a. Fall armyworm FR sequences map to sex chromosomes and their distribution in the world indicate limitations in interstrain mating. Insect Molecular Biology 12 (5): 453-456.

NAGOSHI, R. D.; MEAGHER, R. L. 2003b. FR Tandem-Repeat Sequence in Fall armyworm (Lepidoptera: Noctuidae) Host Strains. Annals of the Entomological Society of America 96 (3): 329-335.

NAGOSHI, R. D.; MEAGHER, R. L. 2004. Behaviour and distribution of the two fall armyworm host strains in Florida. Florida Entomologist 87 (4): 440-448.

NAGOSHI, R. D.; MEAGHER, R. L.: NUESSSLY, G.; HALL, D. G. 2007a. Effects of fall armyworm (Lepidoptera: Noctuidae) interstrain mating in wild populations. Environmental Entomology 35 (2): 561-568.

NAGOSHI, R. D.; SILVIE, P.; MEAGHER, R. L.; LOPEZ, L.; MACHADO, V. 2007b. Identification and comparison of fall armyworm (Lepidoptera: Noctuidae) host strains in Brazil, Texas, and Florida. Annals of the Entomological Society of America 100: 394402.

PASHLEY, D. P. 1986. Host-associated genetic differentiation in fall armyworm (Lepidoptera: Noctuidae) a sibling species complex? Annals of Entomological Society of America 79: 898-904.

PASHLEY, D. P.; MARTIN, J. A. 1987. Reproductive incompatibility between host strains of the fall armyworm (Lepidoptera: Noctuidae). Annals of Entomological Society of America 80: 731–733.

PASHLEY, D. P.; HAMMOND, A. M.; HARDY, T. N. 1992. Reproductive isolating mechanisms in fall armyworm host strains (Lepidoptera: Noctuidae). Annals of Entomological Society of America 85: 400-405.

PASHLEY, D. P. 1998. Sex linkage and speciation in Lepidoptera. pp. 309-319. In Howard, D.; Berlocher, S. (eds). Endless forms: species and speciation. Oxford. NY.

PASHLEY, D. P.; MCMICHAEL M.; SILVAIN J. F. 2004. Multilocus genetic analysis of host use, introgression and speciation in host strains of fall armyworm (Lepidoptera: Noctuidae). Annals of Entomological Society of America 97 (5): 1034-1044.

PINZÓN, G. 1992. Especies sibling de Spodoptera frugiperda. Departamento de desarrollo y registros CIBA-GEYGI COLOMBIANA. 38-41 p

TORRES, L.; COTES, A. M. 2005. Efecto de la crioconservación sobre la viabilidad y actividad biocontroladora de Nomuraea rileyi contra Spodoptera frugiperda (Lepidoptera: Noctuidae). Revista Colombiana de Entomología 31 (2): 133-138.

SAMBROOK, J.; RUSELL, D. 2001. Molecular Cloning. A Laboratory Manual. 3ª. Ed. Cold Spring Harbor Laboratory Press. SOKAL, R. R.; ROLHF, F. J. 1995. Biometry. W. H. Freeman and company, NY.

ZENNER DE POLANÍA, I.; MADONADO, H. A.; MEJIA CRUZ, R. A.; BAYONA, M. 2007. Susceptibilidad de algunas plagas (Lepidoptera, Noctuidae) del algodonero al gene Cry1ac del Bacillus thuringiensis, pp. 147. En: Resúmenes del XXXIV Congreso Colombiano de Entomología.

Authors retain the copyright on their work and are responsible for the ideas expressed in them. Once a manuscript is approved for publication, authors are asked for a publication license for the term of legal protection, for all territories that allows the use, dissemination and disclosure of the same.