Determination of the median lethal concentration (LC50) of mycoinsecticides for the control of Ceratitis capitata (Diptera: Tephritidae)
Main Article Content
The aim of this study was to determine the median lethal concentration (LC50) of the commercial products Boveril WP® (Beauveria bassiana) and Metarril WP® (Metarhizium anisopliae) on the larvae and pupae of the fruit fly, Ceratitis capitata. Insects used in this study came from a laboratory colony. The evaluated product concentrations were 10.00, 15.00, 20.00 and 25.00 g/L of water, which correspond, respectively, to 5.00x109, 7.50x109, 10.00x109 and 12.50x109 viable conidia/L of water for the two products, and in the control only water was applied. Third instar larvae and pupae of C. capitata were used in this study. Results showed an overall mortality of larvae with all conidial concentrations of M. anisopliae. The LC50 values for larvae were 2.99 and 2.97 g/L for Boveril® and Metarril®, respectively, while for pupae they were 3.12 and 4.74 g/L for Boveril® and Metarril®, respectively. The high pathogenicity demonstrated by lower conidial concentrations of the tested products may mean greater efficiency from both economic and environmental points of view.
ABBOTT, W. S. 1925. A method of computing the effectiveness of an insecticide. Journal of Economic Entomology 18: 265-267.
ALMEIDA, G. B.; PRATISSOLI, D.; POLANCZYK, R. A.; HOLTC, A. M.; VICENTINI, V. B. 2007. Determinação da concentração letal média (CL50) de Beauveria bassiana para o controle de Brevicoryne brassicae. Idesia 25: 69-72.
ALVES, S. B. 1992. Perspectivas para utilização de fungos entomopatogênicos no controle de pragas no Brasil. Pesquisa Agropecuária Brasileira 27: 77-86.
ALVES, S. B.; ROSSI, L. S.; WALDER, J. M.; VIEIRA, S. A. 2004. Avaliação de fungos entomopatogênicos para Ceratitis capitata. Manejo Integrado de Plagas y Agroecología 72: 31-38.
BRIDGE, P. D.; ABRAHAM, Y. J.; CORNISH, M. C.; PRIOR, C.; MOORE, D. 1990. The chemotaxonomy of Beauveria bassiana (Deuteromycotina: Hyphomycetes) isolated from coffe berry borer Hypothenemus hampei (Coleoptera: Scolytidae). Mycology 111: 85-90.
CARVALHO, R. S.; NASCIMENTO, A. S. 2002. Criação e utilização de Diachasmimorpha longicaudata para controle biológico de moscas-das-frutas (Tephritidae), pp. 165-179. In: Parra, J. R. P.; Botelho, P. S. M.; Correa-Ferreira, B.; Bento, J. M. S. Controle biológico no Brasil: parasitóides e predadores Editora Manole. 609 p.
CASTILLO, M. A.; MOYA, P.; HERNÁNDEZ, E.; PRIMOYÚFERA, E. 2000. Susceptibility of Ceratitis capitate Wiedmann (Diptera: Tephritidae) to entomopathogenic fungi and their extracts. Neotropical Entomology 19: 274-282.
DIMBI, S.; MANIANIA, N. K.; LUX, S. A.; EKESI, S.; MUEKE, J. K. 2003. Pathogenicity of Metarhizium anisopliae (Metsch.) Sorokin and Beauveria bassiana (Balsamo) Vuillemin, to three adult fruit by species: Ceratitis capitate (Weidemann), C. rosa var. fasciventris Karsch and C. cosyra (Walker) (Diptera: Tephritidae). Mycopathologia 156: 375-382.
EKESI, S.; MANIANIA, N. K.; LUX, S. A. 2002. Mortality in three african tephritid fruit by puparia and adults caused by the entomopathogenic fungi, Metarhizium anisopliae and Beauveria bassiana. Biocontrol Science Technology 12: 7-17.
FINNEY, D. J. 1971. Probit analysis: a statistical treatment of the sigmoid response curve. Cambridge University Press. Cambridge. 246 p.
FRANÇA, I. W. B.; MARQUES, E. J.; TORRES, J. B.; OLIVEIRA, J. V. 2006. Efeitos de Metarhizium anisopliae (Metsch.) Sorok. e Beauveria bassiana (Bals.) Vuill. sobre o percevejo predador Podisus nigripisnus (Dallas) (Hemiptera: Pentatomidae). Neotropical Entomology 35: 349-356.
GALLO, D.; NAKANO, O.; SILVEIRA NETO, S.; CARVALHO, R. P. L.; BAPTISTA, G. C.; BERTI FILHO, E. PARRA, J. R. P.; ZUCCHI, R. A.; ALVES, S. B.; VENDRAMIM, J. D.; MARCHINI, L. C.; LOPES, J. R. S.; OMOTO, C. 2002. Entomologia agrícola. Piracicaba: Fealq, 920 p.
GRIJALBA, B.; VILLAZAR, E. P.; COTES, A. M. 2009. Evaluación de la estabilidad de Paecilomyces sp. y Beauveria bassiana frente a la radiación ultravioleta. Revista Colombiana de Entomología 35 (1): 1-6.
IGNOFFO, C. M. 1992. Environmental factors affecting persistence of entomopathogens. Florida Entomologist 75: 516-525.
KAAYA, G. P.; MUNYINYI, D. M. 1995. Biocontrol potential of the entomogenous fungi Beauveria bassiana and Metarhizium anisopliae for tsetse flies (Glossina spp.) at developmental sites. Journal of Invertebrate Pathology 66: 237-241.
LOUREIRO, E. S.; MOINO JR., A. 2006. Patogenicidade de fungos hifomicetos aos pulgões Aphis gossypii Glover e Myzus persicae (Sulzer) (Hemiptera: Aphididae). Neotropical Entomology 35: 660-665.
MOCHI, D. A.; MONTEIRO, A. C.; BORTOLI, A. S.; DÓRIA, H. O. S.; BARBOSA, J. C. 2006. Pathogenicity of Metarhizium anisopliae for Ceratitis capitate (Wied.) (Diptera: Tephritidae) in soil with different pesticides. Neotropical Entomology 35: 382-389.
OLIVEIRA, F. Q. 2010. Biological control of fruit flies. Revista Verde de Agroecologia e Desenvolvimento Sustentável 5: 23-
OLIVEIRA, F. Q.; MALAQUIAS, J. B.; FERREIRA, L. L.; RIBEIRO, T. S.; PEREIRA, A. I. A. 2010a. Efficacy of molasses and Beauveria bassiana (Balsamo) Vuill on the control of Cosmopolites sorditus Germar, 1824. Engenharia Ambiental (Unipinhal) 7: 127-132.
OLIVEIRA, F. Q.; BATISTA, J. L.; MALAQUIAS, J. B. 2010b. Biological control of fruit flies with entomopathogenic fungi, pp. 187-192. In: Seabra, G.; Novaes da Silva, J. A.; Mendonça, I. T. L. (eds). A Conferência da Terra Fórum Internacional do Meio Ambiente. João Pessoa: Editora Universitária da UFPB.
OMOTO, C.; DENNEHY, C. J.; MCCOY, C. W.; CRANE, S. E.; LONG, J. W. 1994. Detection and characterization of the interpopulation variation of citrus rust mite (Acari: Eriophyidae) resistance to dicofol in Florida citrus. Journal of Economic Entomology 87: 566-572.
ONOFRE, S. B.; VARGAS, L. R. B.; ROSSATO, M.; BARROS, N. M.; BOLDO, J. T.; NUNES, A. R. F; AZEVEDO, J. L. 2002. Controle biológico de pragas na agropecuária por meio de fungos entomopatogênicos, pp. 297-347. In: Serafini, L. A.; Barros, N., M., Azevedo, J. L. (eds.) Biotecnologia na agricultura e na agroindústria. Guaíba. Livraria e Editora Agropecuária 463pp.
PEREIRA, R. M.; STIMAC, J. L.; ALVES, S. B. 1993. Soil antagonism affecting the dose-response of workers of the red imported fire ant Solenopsis invicta, to Beauveria bassiana conidia. Journal Invertebrate Pathology 61: 156-161.
QUESADA-MORAGA, E.; RUIZ-GARCIA, A.; SANTIAGO-ÁLVAREZ, C. 2006. Laboratory evaluation of entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae against puparia and adults of Ceratitis capitate (Diptera: Tephritidae). Journal of Economic Entomology 26: 1955-1966.
REYS, E. G. 2003. Effect de la temperatura, humedad relativa y humedad de suelo, sobre la patogenicidade de Metarhizium anisopliae (Hyphomycetes) em larvas de Anastrepha ludens (Diptera: Tephritidae). Tesis Doctorado em Ciencias: Biotecnologia. Universidad de Colima. 165 p.
SAS/STAT User’s Guide, (2003). Cary, NC: SAS Institute Inc. SILVA, C. A. D. 2001. Seleção de isolados de Beauveria bassiana patogênicos ao bicudo do algodoeiro. Pesquisa Agropecuária Brasileira 36: 243–247.
STEINKRAUS, D. C.; GEDEN, C. J.; RUTZ, D. A.; KRAMER, J. P. 1990. First report of the natural occurrence of Beauveria bassiana (Moniliales: Moniliaceae) in Musca domestica (Diptera: Muscidae). Journal of Medical Entomology 27: 309-312
- FLÁVIA QUEIROZ DE OLIVEIRA, JACINTO DE LUNA BATISTA, JOSÉ BRUNO MALAQUIAS, CARLOS HENRIQUE DE BRITO, EMMERSON PEREIRA DOS SANTOS, Susceptibility of the predator Euborellia annulipes (Dermaptera: Anisolabididae) to mycoinsecticides , Revista Colombiana de Entomología: Vol. 37 No. 2 (2011)
- JOCÉLIA GONÇALVES DA SILVA, JACINTO DE LUNA BATISTA, JOÁLISSON GONÇALVES DA SILVA, CARLOS HENRIQUE DE BRITO, Use of vegetable oils in the control of the citrus black fly, Aleurocanthus woglumi (Hemiptera: Aleyrodidae) , Revista Colombiana de Entomología: Vol. 38 No. 2 (2012)
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Authors retain the copyright on their work and are responsible for the ideas expressed in them. Once a manuscript is approved for publication, authors are asked for a publication license for the term of legal protection, for all territories that allows the use, dissemination and disclosure of the same.