Molecular identification of forensically significant beetles (Coleoptera) in China based on COI gene

https://doi.org/10.25100/socolen.v37i1.9050
Forensic entomology Sarcosaphagous beetles Mitochondrial DNA

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Published: Jan 15, 2011
Issue: Vol. 37 No. 1 (2011)
Section Agriculture / Research paper

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Authors

QUAN ZHUANG Central South University, School of Basic Medical Sciences
JIFENG CAI Central South University, School of Basic Medical Sciences
MENGQI ZHANG Central South University, School of Basic Medical Sciences
HUA FENG Beijing Public Security Bureau
YADONG GUO Central South University, School of Basic Medical Sciences
LINGMEI LAN Central South University, School of Basic Medical Sciences
YAOQING CHEN Changsha Public Security Bureau in Hunan
Abstract

Precise identification of insect species plays an essential role in the accurate estimation of the postmortem interval (PMI), especially when information on the postmortem phenomenon is not available. Sarcosaphagous beetles infest and colonize human and animal remains in the late stage of decomposition, and their morphological similarity poses a great challenge for forensic entomologists, as an existing key may be incomplete or difficult for non-specialists to use. A method for easy and accurate species-level identification at any life stage is required. In this study, a 272-base pair region of the mitochondrial cytochrome oxidase I (COI) gene was used to explore its utility in the identification of forensically important beetles. Twenty-four specimens were collected from 14 locations in nine provinces of China. Phenogram analysis of the sequenced segments by the unweighted pairgroup method analysis (UPGMA) method showed that all specimens were properly assigned into six species with strong similarity, which indicates the possibility of separating congeneric species with the short COI fragment. These results will be instrumental for implementation of the Chinese database of forensically relevant beetles.

How to Cite
ZHUANG, Q., CAI, J., ZHANG, M., FENG, H., GUO, Y., LAN, L., & CHEN, Y. (2011). Molecular identification of forensically significant beetles (Coleoptera) in China based on COI gene. Revista Colombiana De Entomología, 37(1), 95–102. https://doi.org/10.25100/socolen.v37i1.9050
References

ALESSANDRINI, F.; MAZZABTI, M.; ONOFRI, V.; TURCHI, C.; TAGLIABRACCI, A. 2008. MtDNA analysis for genetic identification of forensically important insects. Forensic Science International: Genetics Supplement Series 1 (1): 584-585.

AMENDT, +J.; KRETTEK, R.; ZEHNER, R. 2004. Forensic entomology. Naturwisseschaften 2 (91): 51-65.

ANDERSON, G.S. 2000. Insect succession on carrion and its relationship to determining time of death, in: J.H. Byrd, J.L. Castner (Eds.), Forensic Entomology. The utility of arthropods in legal investigations. Boca Raton. CRC Press. 143-175 p.

ARNOLDI, F. G.; OGOH, K.; OHMIYA, Y.; VIVIANI, V. R. 2007. Mitochondrial genome sequence of the Brazilian lumines cent click beetle Pyrophorus divergens (Coleoptera Elateridae): mitochondrial genes utility to investigate the evolutionary history of Coleoptera and its bioluminescence. Gene 405 (1-2): 1-9.

AVISE, J. C. 2000. Phylogeography: The history and formation of species. Harvard University Press, Cambridge. vii+453 p.

BENECKE, M. 2008. A brief survey of the history of forensic entomology. Act Biologica Beurodis 14:15-38.

BYRD, J. H.; CASTNER, J. L. 2000. Entomological evidence: utility of arthropods in legal investigations. Boca Raton. CRC Press. xxiii+663p.

CATTS, E. P. 1992. Problems in estimating the PMI in death investigations. Journal of Agricultural Entomology 37 (9): 253-272.

CROZIER, R. H.; CROZIER, Y. D. 1993. The mitochondrial genome of the honeybee Apis mellifera: complete sequence and genome organization. Genetics 113 (1): 97-117.

DANIEL, J. F. 1999. Molecular systematics of cytochrome oxidase I and 16s from Neochlamisus leaf beetle and the importance of sampling. Molecular Biology and Evolution 16 (1): 67-82.

DAVID, C. M.; MICHAEL, P. S.; STEVEN, J. B.; COOPER, S. J.; LAURENCE, A. M. 2002. Phylogenetics of Australian Acacia thrips: the evolution of behaviour and ecology. Molecular Phylogenetics and Evolution 25 (2): 278-292.

DAVID, J. R.; BRENT, C. E.; PEDRO, O.; GODFREY, M. H. 2001. The Diversification of the Genus Nesotes (Coleoptera: Tenebrionidae) in the Canary Islands: Evidence from mtDNA. Molecular Phylogenetics and Evolution 21(2): 321-326.

DIRK, A.; MICHAEL, T. M.; ALFRIED, P. V. 2007. DNA-based taxonomy for associating adults and larvae in multi-species assemblages of chafers (Coleoptera: Scarabaeidae). Molecular Phylogenetics and Evolution 44 (1): 436-449.

ELENA, M.; MARTINEZ, N.; JOSE, G.; JOSE, S. 2005. Phylogeny and molecular evolution of the tribe Harpalini (Coleoptera, Carabidae) inferred from mitochondrial cytochrome-oxidase I. Molecular Phylogenetics and Evolution 35 (1): 127-146.

FANG, X. M. 2009. Phylogenetic analysis of Chrysomelidae in China inferred from mitochondrial COI sequences (Coleoptera: Chrysomeloidea). Journal of Suzhou University 24 (1): 113-117.

FRIEDRIC, M.; MUQIM, N. 2003. Sequence and phylogenetic analysis of the complete mitochondrial genome of the flour beetle Tribolium castaneum. Molecular Phylogenetics and Evolution 26 (3): 502-512.

GOFF, M.; CATTS, P. 1990. Arthropods basic structure and biology. In Catts, P.; Haskell, N. (eds.). Entomological and death a procedural guide. Joyce´s Print Shop. 38.71.

GREENBERG, B. 2002. Key to the eggs, larvae, pupae and adults of some forensically important flies, pp. 54–153. In: B. Greenberg & J. C. Kunich (eds). Entomology and the law flies as forensic indicators. Cambridge University Press, Cambridge. xii+298 p. GUO, Y. D.; CAI , J. F.; WANG, X. H.; LAN, L. M.; LIU, Q. L.; LI, X.; CHANG, Y. F.; ZHONG, M.; WANG, X.;WEN, J. F. 2010. Identification of forensically important sarcophagid flies (Diptera: Sarcophagidae) based on COI gene in China. Romanian Journal of Legal Medicine 18 (3): 217-224.

HARVEY, M. L.; DADOUR, I. R.; GAUDIERI, S. 2003. Mitochondrial DNA cytochrome oxidase I gene: potential for distinction between immature stages of some forensically important fly species (Diptera) in western Australia. Forensic Science International 131(2-3): 134-139.

HASKELL, N. H.; HALL, R. D.; CERVANKA, V. J. 1997. On the body: Insects’ life stage presence and their postmortem artifacts. pp. 415-448. Forensic taphonomy: The post mortem fate of human remains. Boca Raton. CRC Press, Florida. xxvi+636 p.

JUDITH, A. S.; NICOLA, C. B. 2008. Molecular genetic identification of forensically important flies in the UK. Forensic Science International: Genetics Supplement Series 1 (1): 620-622. KIM, I.; LEE, S. C.; JIN, B. S. 2000. Genetic divergence and phylogenetic relationships among the Korean fireflies, Hotaria papariensis, Luciola lateralis, and Pyrocoelia rufa (Coleoptera: Lampyridae), using mitochondrial DNA sequences. Korean Journal of Applied Entomology 39 (4): 211-226.

LAN, L. M.; LIAO, Z. G.; CHEN, Y. Q.; YAO, Y.; LI, J. B.; LI, M. O.; CAI, J. F. 2006. Advances of forensic entomology in China.Journal of Forensic Medicine 22 (6): 448-450.

LECLERCQ, M.; LECOMTE, J. 1978. Emergency treatment of insect bite complications. Revue Médicale de Liège 33: 240 249.

LEE, S. C.; BAE, J. S.; KIM, I.; SUZUKI, H.; KIM, S. R.; KIM, J. G.; KIM, K. Y.; YANG, W. J.; LEE, S. M.; SOHN, H.D.; JIN, B. R. 2003. Mitochondrial DNA sequence-based population genetic structure of the firefly, Pyrocoelia rufa (Coleoptera: Lampyridae). Biochemical Genetics 41 (11-12): 427-452.

LI, X.; OGOH, K.; OHBA, N.; LIANG, X.; OHMIYA, Y. 2007. Mitochondrial genomes of two luminous beetles, Rhagophthalmuslufengensis and Rohbai (Arthropoda, Insecta, Coleoptera). Gene 392 (1-2) 196-205.

LIU, Q. L.; CAI, J. F.; GUO, Y. D.; WANG, X.H.; GU, Y.; WEN, J.F.; MENG ,F.M.; YI, W.P. 2011. Letter to Editor. Identification of forensically significant calliphorids based on mitochondrial DNA cytochrome oxidase I (COI) gene in China. Forensic Science International, in press.

LU, B. L.; WU, H. Y. 2003. Classification and identification of important medical insects of China. Henan Science and Technology Publishing House, Zhengzhou. 800 p.

LUNT, D. H.; ZHANG, D. X.; SZYMURA, J. M.; HEWITT, G. M. 1996. The insect cytochrome oxidase I gene: evolutionary patterns and conserved primers for phylogenetic studies. Insect Molecular Biology 5 (3): 153-165.

OZDEMIR, S.; SERT, O. 2009. Determination of Coleoptera fauna on carcasses in Ankara province, Turkey. Forensic Science International 183 (1-3): 24-32.

PAUL, F.; RUGMAN, J.; ROBERT, W.; TOM, V. N.; RICHARD, S. 2009. Molecular differentiation of the Psyttalia concolor (Szépligeti) species complex (Hymenoptera: Braconidae) associated with olive fly, Bactrocera oleae (Rossi) (Diptera: Tephritidae), in Africa. Biological Control 49: 17-26.

PENG, Q. Y.; YE, L. S.; MA, L. P.; CAI, J .F. 2009. The succession of sarcophagus beetles on carrion and its application in forensic medicine. Journal of Forensic Medicine 25 (6): 447-450.

SAIGUSA, K.; TAKAMIYA, M.; MATSUMASA, M.; AOKI, Y. 2006. The forensic availability of a simple and time-saving method for the identification of dipteran species to estimate postmortem interval using entomological evidence. Legal Medicine 8(4): 252-254

SIMON, C.; FRATI, F.; BECKENBACH, A.; CRESPI, B.; LIU, H.; LOOK, P. F. 1994. Evolution, weighting and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Annals of the Entomological Society of America 87 (6): 651-701.

SKEVINGTON, J. H.; YEATES, D. K. 2000. Phylogeny of the Syrphoidea (Diptera) inferred from mtDNA sequences and morphology with particular reference to classification of the Pipunculidae (Diptera). Molecular Phylogenetics and Evolution 16 (2): 212-224.

SNEATH, P.; SOKAL, R. 1973. Numerical Taxonomy. Freeman, San Francisco.

STIJIN, D; MATTHIAS, G. 2009. COI sequence variability between Chrysomyinae of forensic interest. Forensic Science International: Genetics 3 (2): 89-95.

SU, Z. H.; IMURA, Y. K.; MUNEHIRO, O.; SYOZO, O. 2004. Pattern of phylogenetic diversification of the Cychrini ground beetles in the world as deduced mainly from sequence comparisons of the mitochondrial genes. Gene 326:43-57.

SUKONTASON, K.; NARONGCHAI, P.; KANCHAI, C. 2007. Forensic entomology cases in Thailand: a review of cases from

to 2006. Parasitology Research 101(5): 1417-1423.

SUZUKI, H.; SATO, Y.; OHBA, N. 2002. Gene diversity and geographic differentiation in mitochondrial DNA of the GeUPGMAi firefly, Luciola cruciate (Coleoptera: Lampyridae). Molecular Phylogenetics and Evolution 22 (2): 193-205.

SUZUKI, H.; SATO, Y.; OHBA, N.; BAE, J. S.; JIN, B. R.; SOHN, H. D.; KIM, S. E. 2004. Phylogenetic analysis of the firefly, Luciola lateralis, in Japan and Korea based on mitochondrial cytochrome oxidase II gene sequences (Coleoptera: Lampyridae). Biochemical Genetics 42 (9-10): 287-300.

TAMURA, K.; DUDLEY, J.; NEI, M.; KUMAR, S. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution 24 (8):15961599.

WALLMAN, J. F.; DONNELLAN, S. C. 2001. The utility of mitochondrial DNA sequences for the identification of forensically important blowflies (Diptera: Calliphoridae) in southeastern Australia. Forensic Science International 20(1-2):60-67.

WANG, B. Q.; CAI J. F.; GE, Y.; LI, F. Z.; MAN, Y.; CHANG, Y. F. 2008. Advances of the study of sarcosaphagous insects in forensic medicine. Journal of Forensic Medicine 24 (3): 210-213.

WELLS, J. D.; SPERLING, F. A. 2001. DNA-based identification of forensically important Chrysomyinae (Diptera: Calliphoridae). Forensic Science International 120 (1-2): 110-115.

WELLS, J. D.; WALL, R.; STEVENS, J. R. 2007. Phylogenetic analysis of forensically important Lucilia flies based on cytochrome oxidase I sequence: a cautionary tale for forensic species determination. International Journal of Legal Medicine 121(3): 229-233.

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