Entomopathogenic nematodes (Rhabditida: Heterorhabditidae and Steinernematidae) screening for lesser mealworm Alphitobius diaperinus (Coleoptera: Tenebrionidae) control
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Alphitobius diaperinus (Coleoptera: Tenebrionidae), an important poultry insect pest, is difficult because of the insect’s behavior and biology and the restrictions for the used of synthetic insecticides. So, entomopathogenic nematodes are an alternative for A. diaperinus control. This study aimed to screen in laboratory entomopathogenic nematodes for A. diaperinus control. Fourteen strains were tested, and for the best LC50 and LC90 was estimated, as well as the control efficiency on adult insects at different temperatures (18, 22, 26, 30 and 35ºC). The poultry litter and sanitary products effect on nematodes were also evaluated. All strains were pathogenic with mortality values varying from 1 to 99%. Steinernema arenarium was the most effective causing 99% mortality. The estimated LC90 was 106 infective juveniles (IJ) per insect (approximately 25 IJ/cm2). No nematodes activity was observed at temperatures above 30°C. The antagonistic action of poultry litter inhibit completely the effect on adult insects and a reduction on larvae control by 57%.
ABREU, V. M. N.; PAIVA, D. P.; ABREU, P. G.; COLDEBELLA, A. 2011. Evaluation of litter material and ventilation systems in poultry production: III. Litter reuse, darkling beetle populations and intestinal parasites. Revista Brasileira de Zootecnia 40: 13721380.
ALVES, L. F. A.; ALVES, V. S.; BRESSAN, D. F.; NEVES, P. M. O. J.; ALVES, S. B. 2004. Ocorrência de Metharizium anisopliae (Metsch) Sorok. Em adultos de cascudinho (Alphitobius diaperinus) (Panzer) (Coleoptera: Tenebrionidae) em aviários comerciais em Cascavel, PR. Neotropical Entomology 33: 793-795.
ALVES, L. F. A.; GASSEN, M. H.; PINTO, F. G. S.; NEVES, P M. O. J.; ALVES, S. B. 2005a. Ocorrência natural de Beauveria bassiana (Bals.) Vuilleman (Moliniales: Moniliaceae) sobre o cascudinho (Alphitobius diaperinus) (Panzer) (Coleoptera: Tenebrionidae), em aviário comercial em Cascavel, PR. Neotropical Entomology 34: 507-510.
ALVES, L. F. A.; ROHDE, C.; ALVES, V. S. 2005b. Patogenicidade de Steinernema glaseri e S. carpocapsae (Nematoda: Rhabdita) contra o cascudinho, Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae). Neotropical Entomology 34: 139-141.
ALVES, L. F. A.; NEVES, P. M.O. J.; OLIVEIRA, R. C.; OLIVEIRA, D. G. P. 2008. Fatores a serem considerados na utilização de Beauveria bassiana visando o manejo de populações de Alphitobius diaperinus em aviários comerciais. Arquivos do Instituto Biológico 75: 13-20.
ALVES, V. S.; MOINO, JR. A.; SANTA-CECILIA, L. V. C.; ANDALÓ, V.; SOUZA, G. C. 2009. Patogenicidade de nematóides entomopatogênicos a cochonilha-da-raiz-do-cafeeiro Dysmicoccus texensis (Tinsley) (Hemiptera: Pseudococcidae) em laboratório. Arquivos do Instituto Biológico 76: 67-73.
BACON, C. W. 1986. Effects of broiler litter volatiles and ammonia on fungal spore germination. Poultry Science 65: 710-716.
CHERNAKI-LEFFER, A. M. 2004. Dinâmica populacional, estimative da resistência a inseticidas e suscetibilidade do cascudinho Alphitobius diaperinus (Panzer, 1797) (Coleoptera: Tenebrionidae) a inseticidas reguladores de crescimento e a fungos entomopatogênicos. Tese (Pós-Graduação em Ciências Biológicas)– Universidade Federal do Paraná – UFPR. 128 p.
CHERNAKI-LEFFER, A. M.; LAZZARI, F. A.; LAZZARI, S. M.; ALMEIDA, L. M. 2001. Controle do cascudinho. Avicultura Industrial 1094: 22-25.
CHERNAKI-LEFFER, A. M.; BIESDORF, S. M.; ALMEIDA, L. M.; LEFFER, E. V. B.; VIGNE, F. 2002. Isolamento de enterobactérias em Alphiobius diaperinus e na cama de aviários no oeste do estado do Paraná, Brasil. Revista Brasileira de Ciência Avícola 4: 243-247.
CHERNAKI-LEFFER, A. M.; SOSA-GOMEZ, D. R.; ALMEIDA, L. M. 2007. Selection for entomopathogenic fungi and LD50 of Metarhizium anisopliae (Metsch.) Sorok. for the Lesser Mealworm Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae). Revista Brasileira de Ciência Avícola 9: 209-213.
CHINIVASAGAM, H.N., REDDING, M., RUNGE, G., BLACKALL, P.J. 2010. Presence and incidence of food-borne pathogens in australian chicken litter. British Poultry Science 51: 311-318.
DESPINS, J. L.; AXTELL, R. C. 1995. Feeding behavior and growth of broiler chicks fed larvae of the darkling beetle, Alphitobius diaperinus. Poultry Science 74 (2): 331-336.
FERREIRA, D. F. 2003. Manual do sistema Sisvar para análises estatisiticas. Disponível em: <http://www.dex.ufla.br/danielff/sisvarmanual.pdf> [Review date: 25 apr 2006].
GEDEN, C. J.; ARENDS, J. J.; BROOKS, W. M. 1985. Susceptibility of the lesser mealworm, Alphitobius diaperinus (Coleptera: Tenebrionidae) to the entomogenous nematodes Steinernema feltiae, S. glaseri (Steinernematidae) and Heterorhabditis heliothidis (Heterorhabditidae). Journal of Entomology Science 20: 331-339.
GEDEN, C. J.; ARENDS, J. J.; AXTELL, R. C. 1987. Field trials of Steinernema feltiae (Nematoda: Steinernematidae) for control of Alphitobius diaperinus (Coleoptera: Tenebrionidae) in commercial broiler and turkey houses. Journal of Economic Entomology 80: 136-141.
GEDEN, C. J.; AXTELL, R. C. 1988. Effect of temperature on nematode (Steinernema feltiae [Nematoda: Steinernematidae]) treatment of soil for control of lesser mealworm (Coleoptera: Tenebrionidae) in turkey houses. Journal of Economic Entomology 81: 800-803.
GEDEN, C. J.; STEINKRAUS, D. C. 2003. Evaluation of three formulations of Beauveria bassiana for control of lesser mealworm and hide beetle in Georgia poultry houses. Journal of Economic Entomology 96: 1602-1607.
HADDAD, M. L., 1998. Utilização do Polo-PC para análise de Probit. pp. 999-1013. In: Alves, S. B. (Ed.). Controle microbiano de insetos. FEALQ, Piracicaba.
HAZELEGER, W. C.; BOLDER, N. M.; BEUMER, R. R.; JACOBS-REITSMA, W. F. 2008. Darkling beetles (Alphitobius diaperinus) and their larvae as potential vectors for the transfer of Campylobacter jejuni and Salmonella enteric serovar paratyphi B variant java between successive broiler flocks. Applied Environmental Microbiology 74: 6887-6891.
JAAP, A. K.; BICHO, C. L.; SILVA, A.V. F. 2010. Importância e medidas de controle de Alphitobius diaperinus em aviários. Ciência Rural 40: 1668-1673.
MATIAS, R. S. 1992. Controle de Alphitobius diaperinus em piso e cama de aviários. Pesquisa Agropecuária Brasileira 27: 205-207. OLIVEIRA, R. C. 2005. Influência do substrato e a utilização de armadilhas com Beauveria bassiana e inseticida no controle de Alphitobius diaperinus. Tese de Doutorado. Universidade Estadual de Londrina UEL. 66 p.
PINTO, D. M.; RIBEIRO, P. B.; BERNARDI, E. 2005. Flutuação populacional de Alphitobius diaperinus (Panzer, 1979) (Coleoptera: Tenebrionidae), capturados por armadilhas do tipo sanduíche, em granja avícola, no município de Pelotas, RS. Arquivos do Instituto Biológico 72: 199-203.
POINAR, G. O. Jr. 1979. Nematodes for biological control of insects. Boca Raton, Florida: CRC Press.
RODRIGUEIRO, T. S. C.; GINARETE, C. M. A.; LEITE, L. G.; TAVARES, F. M.; GOULART, R. M.; GIACOMETTI, F. H. C. 2008. Eficiência de Heterorhabditis indica IBCB-N05 (Rhabditida: Heterorhabditidae) no controle de Alphitobius diaperinus (Coleoptera: Tenebrionidae) sob comedouros de granja avícola. Arquivos do Instituto Biológico 75: 279-284.
ROHDE, C.; ALVES, L. F. A.; NEVES, P. M. O. J.; ALVES, S. B.; DA SILVA, E. R. L.; ALMEIDA, J. E. M. 2006. Seleção de Isolados de Beauveria bassiana (Bals.) Vuill. e Metarhizium anisopliae (Metsch.) Sorok. contra o Cascudinho Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae). Neotropical Entomology 35: 231-240.
STEELMAN, D. 1996. Darkling beetles are costly pests. Poultry Digest 55: 22-23.
STEINKRAUS, D. C.; GEDEN, C. J.; RUTZ, D. A. 1991. Susceptibility of lesser mealworm (Coleoptera: Tenebrionidae) to Beauveria bassiana (Moniliales: Moniliaceae): effects of host stage, substrate, formulation, and host passage. Journal of Medical Entomology 28: 314-321.
SZALANSKI, A. L.; PALMER, T. W.; MCKAY, T.; STEELMAN, C. D. 2004. Infectivity of Steinernema spp. (Nematoda: Steinernematidae) to adult litter beetles, Alphitobius diaperinus (Coleoptera: Tenebrionidae) in the laboratory. Biocontrol Science Technology 14: 81-85.
SZCZEPANIK, M. 2000. Temperature effects on the efficacy of nematodes against the lesser mealworm Alphitobius diaperinus (Coleoptera: Tenebrionidae). Polskie Pismo Entomologiczne 69: 483-490.
VITTORI, J.; SCHOCKEN-ITURRINO, R. P.; TROVÓ, K. P.; RIBEIRO, C. A. M.; BARBOSA, G. G.; SOUZA, L. M.; PIGATTO, C. P. 2007. Alphitobius diaperinus as a vector of Clostridium perfrigens in broiler houses in the state of São Paulo Brazil. Ciência Rural 37: 894-896.
WATSON, D. W.; GUY, J. S.; STRINGHAM, S. M. 2000. Limited transmission of turkey coronavirus in young turkeys by adult Alphitobius diaperinus (Coleoptera: Tenebrionidae). Journal of Medical Entomology 37: 480-483.
- Bruna A. Guide, Elaine A. Soares, Camila R. B. Itimura, Viviane S. Alves, Nematodos entomopatogénicos para el control de la cochinilla de la raíz de la yuca, Dysmicoccus sp. (Hemiptera: Pseudococcidae) , Revista Colombiana de Entomología: Vol. 42 No. 1 (2016)
- VANESSA ANDALÓ, ALCIDES MOINO JR, CLEBER MAXIMINIANO, VICENTE P. CAMPOS, LÚCIA A. MENDONÇA, Influence of temperature and duration of storage on the lipid reserves of entomopathogenic nematodes , Revista Colombiana de Entomología: Vol. 37 No. 2 (2011)
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