Microsatellite identification associated with resistance to the mite Mononychellus tanajoa (Acari: Tetranychidae)
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The development of the cassava genetic map has promoted research such as the finding of genes associated with the resistance of pests that diminish the production and quality of the crop. Molecular tools, such as markers can facilitate the identification of the genes that dominate resistance to pests this is the specific case of the presence of resistance to the green mite Mononychellus tanajoa (CGM) in cassava (Manihot esculenta). Initially, at International Center for Tropical Agriculture CIAT, individuals populations of backcrosses (BC1), lineage of four denominated families CW, were identified. These BC1 were identified and categorized according to 1-5 scale of damage reported by the Entomology Program at International Center for Tropical Agriculture. Resistant and susceptible individuals were identified. A total of 500 microsatellite markers were evaluated using the bulk segregant analysis (BSA). Five of them expressed polymorphism and showed significant differences between parental and contrast groups and three markers, namely, SSRY 11, SSRY 346 and, NS 1099. Statistical analysis revealed that markers NS 1099 and NS 346 displayed the highest association between resistance and the individuals of families CW 67, CW 66 and CW 67, respectively. On the basis of these results we can recommend the use of microsatellite for further analysis in other populations in order to achieve the of some chromosomal regions conferring resistance to green mite cassava.
ALLEM, A. C. 1994. The origin of Manihot esculenta Crantz (Euphorbiaceae). Genetic Resources and Crop Evolution 41: 133-150.
AWOLEYE, F., VAN DUREN, M., DOLEZEL, J., NOVAK, F. J.1994 Nuclear DNA content and in vitro induced somatic polyploidization (Manihot esculenta Crantz) Cassava breeding. Euphytica 76: 195-202.
BELLOTTI, A. C.; SMITH, L.; LAPOINTE, S. T. 1999. Recent advances in cassava pest management. Annual Review of Entomology 44: 343-370.
BURBANO, M.; CARABALÍ, A.; MONTOYA-L., J.; BELLOTTI, A. C. 2007. Resistencia de especies de Manihot a Mononychellus tanajoa (Acariformes), Aleurotrachelus socialis y Phenacoccus herreni (Hemiptera). Revista Colombiana de Entomología 33 (2): 110-115.
DELLAPORTA, S. L.; WOOD, J.; HICKS, J. B. 1983. A plant DNA mini preparation: version II. Plant Molecular Biology Report 1: 19-21.
FAO/FIDA (Fondo Internacional de Desarrollo Agrícola/Organización de las Naciones Unidas para la Agricultura y la Alimentación). 2000. La economía mundial de la yuca: hechos, tendencias y perspectivas. Italia. 78 p.
FREGENE, M.; ÁNGEL, F.; GÓMEZ, R.; RODRÍGUEZ, F.; CHAVARRIAGA, P.; ROCA, W.; TOHME, J.; BONIERBALE, M.1997. A molecular genetic map of cassava (Manihot esculenta Crantz). Theoretical and Applied Genetics 95: 431-441.
HURTADO, P. X.; ÁLVAREZ, E.; FREGENE, M.; LLANO, G. A. 2004. Detección de marcadores microsatélites asociados con la resistencia a Xanthomonas axonopodis pv. manihotis en una familia de yuca (bc1). Fitopatolología Colombiana 28 (2): 81-86.
JOS, J. S., NAIR, S. G. 1979. Pachytene pairing in relation to pollen fertility in five cultivars of Cassava. Cytologia 44: 813-820.
LENIS, J. I.; BRAUN, R.; MESA, N.; DUQUE, M. 1993. Uso de escalas para la estimación de poblaciones de ácaros Tetranychidae en cultivos de yuca. pp 139-172. En: Braun, R. (Ed.). Bases fundamentales para investigación sobre los ácaros plagas y sus enemigos naturales en el Ecuador. Documento de trabajo No 126. CIAT, Cali-Colombia. 51 p.
MICHELMORE, R. W.; PARAN, I.; KESSELI, R. V. 1991. Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proceedings of the National Academy of Sciences of USA 88 (21): 98289832.
MORAES, G.; DENMARK, H.; GUERRERO, J. M. 1982. Phytoseiid mites of Colombia (Acarina: Phytoseiidae). En: International Journal Acarology 8 (1): 15-22.
OKOGBENIN, E.; FREGENE, M. 2001. Genetic mapping of QTLs affecting productivity and plant architecture in a full-sib cross from non-inbred parents in cassava (Manihot esculenta Crantz) Theoretical and Applied Genetics 107: 1452-1462.
OKOGBENIN, E.; MARIN, J.; FREGENE, M. 2006. An SSR-based molecular genetic map of cassava. Euphytica 147: 433-440. OSPINA, B.; CEBALLOS, H. 2002. La yuca en el tercer milenio. Sistemas mordernos de producción, procesamiento y comercialización. CLAYUCA. 586 p. (Publicacion CIAT; No. 327) ISBN 958-694-043-8. Cali. Colombia.
ROA, A.; MAYA, C.; DUQUE, M. C.; TOHME, J.; ALLEM, A.C.; BONIERBALE, M. W. 1997. AFLP analysis of relationships among cassava and other Manihot species. Theoretical and Applied Genetics 95: 741-750.
- OCTAVIO VARGAS-H., ANTHONY C. BELLOTTI, PERFORMANCE LOSSES CAUSED BY Phenacoccus herreni Cox & Williams IN TWO YUCCA CLONES , Revista Colombiana de Entomología: Vol. 10 No. 3-4 (1984)
- LUIS CARLOS PARDO-LOCARNO, JAMES MONTOYA-LERMA, AART SCHOONHOVEN, Abundance of whitegrubs (Coleoptera: Melolonthidae) in two colombian agroecosystems , Revista Colombiana de Entomología: Vol. 29 No. 2 (2003)
- JOSE A. CASTILLO, ANTHONY C. BELLOTTI, DIAGNOSTIC CHARACTERS OF FOUR SPECIES OF FLOURY LICE (PSEUDOCOCCIDAE) IN YUCA CROPS (Manihot esculenta) AND OBSERVATIONS ON SOME OF THEIR NATURAL ENEMIES , Revista Colombiana de Entomología: Vol. 16 No. 2 (1990)
- CLAUDIA MARÍA HOLGUÍN-A, ARTURO CARABALI, ANTHONY C. BELLOTTI, Intrinsic rate of population increase of Aleurotrachelus socialis (Hemiptera:Aleyrodidae) in cassava Manihot esculenta , Revista Colombiana de Entomología: Vol. 32 No. 2 (2006)
- JORGE E. PEÑA, ANTHONY C. BELLOTTI, STUDIES ON THE FLIES OF THE STEM AND FRUIT OF YUCCA Anastrepha pickeli and Anastrepha manihoti , Revista Colombiana de Entomología: Vol. 3 No. 3-4 (1977)
- ELSA LILIANA MELO-M., CARLOS ALBERTO ORTEGA-OJEDA, ANDREAS GAIGL, RALF-UDO EHLERS, ANTHONY C. BELLOTTI, Evaluation of two commercial strains of entomonematodes as control agents of Cyrtomenus bergi Froeschner (Hemiptera: Cydnidae) , Revista Colombiana de Entomología: Vol. 32 No. 1 (2006)
- MARITZA BURBANO-M., ARTURO CARABALÍ-M., JAMES MONTOYA-L., ANTHONY C. BELLOTTI, Resistance of Manihot species to Mononychellus tanajoa (Acariformes), Aleurotrachelus socialis, and Phenacoccus herreni (Hemiptera) , Revista Colombiana de Entomología: Vol. 33 No. 2 (2007)
- FRANCISCO LÓPEZ-MACHADO, DANIEL C. PECK, JAMES MONTOYA-LERMA, The importance of vibrational communication in the reproductive behavior of grassland spittle bugs (Homoptera: Cercopidae) , Revista Colombiana de Entomología: Vol. 27 No. 1 (2001)
- Anderson Arenas, James Montoya-Lerma , Revisión taxonómica de los géneros de escarabajos tigre neotropicales de la subtribu Odontocheilina (Coleoptera: Cicindelidae) , Revista Colombiana de Entomología: Vol. 47 No. 2 (2021): Servicios Ecosistémicos
- ANA MILENA CAICEDO-V., ANTHONY C. BELLOTTI, Survey of native entomogenous nematodes associated with Cyrtonemus bergi Froeschner (Hemiptera: Cydnidae) in eight Colombian sites , Revista Colombiana de Entomología: Vol. 22 No. 1 (1996)
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