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A new genetic variation and potential new haplotype of Bactericera cockerelli was identified based on the analysis of the mitochondrial region Cytochrome Oxidase subunit I of two populations from greenhouses in Villa Unión, Durango and Saltillo, Coahuila, Mexico. A variation was found in the base 247 of a 500 bp amplified of the mtCOI gene, this change implies the substitution of an adenine (A) to a Guanine (G), this mutation was detected in the insects collect from greenhouses and in their progeny obtained under laboratory conditions. This genetic variant has a great potential to be a new haplotype of B. cockerelli, therefore, it was designated Mexico Haplotype 1 (MXH1) with GenBank accession number KX130767. A total of ten insects from the original collection and then from laboratory cages were evaluated for Lso infection and all tested negative.

Yisa María Ochoa-Fuentes, Universidad Autónoma Agraria Antonio Narro

Profesor Investigador, Departamento de Parasitología Agrícola

Beltrán-Beache, M., Delgado-Ortíz, J. C., Ochoa-Fuentes, Y. M., & Cerna Chávez, E. (2022). Genetic variation of Bactericera cockerelli Šulc. (Hemiptera: Triozidae) suggests new haplotype in México . Revista Colombiana De Entomología, 48(2). https://doi.org/10.25100/socolen.v48i2.11094

ARTIMO, P.; JONNALAGEDDA, M.; ARNOLD, K.; BARATIN, D.; CSARDI, G.; de CASTRO, E.; DUVAUD, S.; FLEGEL, V.; FORTIER, A.; GASTEIGER, E.; GROSDIDIER, A.; HERNÁNDEZ, C.; IOANNIDIS, V.; KUZNETSOV, D.; LIECHTI, R.; MORETTI, S.; MOSTAGUIR, J.; REDASCHI, N.; ROSSIER, G.; XENARIOS, I.; STOCKINGER, H. 2012. ExPASy: SIB bioinformatics resource portal, Nucleic Acids Research 40 (W1): W597-W603. https://doi.org/10.1093/nar/gks400

CERNA, E.; BELTRÁN, M.; OCHOA, Y. M.; HERNÁNDEZ, O.; DELGADO, J. C. 2021. Bactericera Cockerelli Vector de Candidatus Liberibacter Solanacearum, morfometría y haplotipos nn poblaciones de México. Revista Mexicana de Ciencias Agrícolas 26 (Número especial): 81-94. https://doi.org/10.29312/remexca.v0i26.2939

CHAPMAN, R. I.; STRUBE, L.; BEXTINE, B. 2010. Population genetics of the potato psyllid: Impacts on zebra chip epidemiology, pp. 64-68. In Proceedings, 10th Annual Zebra Chip Reporting Session, 7-10 November 2010, Texas AgriLife, College Station, TX.

CROSSLIN, J. M.; LIN, H.; MUNYANEZA, J. E. 2011. Detection of Candidatus Liberibacter solanacearum in the potato psyllid, Bactericera cockerelli (Sulc), by conventional and real-time PCR. Southwestern Entomologist 36 (2): 125-135. https://doi.org/10.3958/059.036.0202

DOYLE, J. J.; DOYLE, J. L. 1990. Isolation of plant DNA from fresh tissue. Focus 12 (1): 13-15. https://www.scirp.org/(S(351jmbntvnsjt1aadkposzje))/reference/ReferencesPapers.aspx?ReferenceID=633608

LIEFTING, L. W.; SUTHERLAND, P. W.; WARD, L. I.; PAICE, K. L.; WEIR, B. S.; CLOVER, G. R. G. 2009. A new ‘Candidatus Liberibacter’ species associated with diseases of solanaceous crops. Plant Disease 93 (3): 208-214. https://doi.org/10.1094/PDIS-93-3-0208

LIU, D.; TRUMBLE, J. T.; STOUTHAMER, R. 2006. Genetic differentiation between eastern populations and recent introductions of potato psyllid (Bactericera cockerelli) into western North America. Entomologia Experimentalis et Applicata 118 (3): 177-183. https://doi.org/10.1111/j.1570-7458.2006.00383.x

MORRIS, J.; SHILLER, J.; MANN, R.; SMITH, G.; YEN, A.; RODONI, B. 2017. Novel ‘Candidatus Liberibacter’ species identified in the Australian eggplant psyllid, Acizzia solanicola. Microbial Biotechnology 10 (4): 833-844. https://doi.org/10.1111/1751-7915.12707

MUNYANEZA, J. E. 2010. Psyllids as vectors of emerging bacterial diseases of annual crops. Southwestern Entomologist 35 (3): 471-477. https://doi.org/10.3958/059.035.0335

MUNYANEZA, J. E.; SENGODA V. G.; GARZÓN-TIZNADO, J. A.; CÁRDENAS-VALENZUELA, O. G. 2009. First Report of "Candidatus Liberibacter solanacearum" in tomato plants in Mexico. Plant Disease 93 (10): 1076. https://doi.org/10.1094/PDIS-93-10-1076A

MUNYANEZA, J. E.; CROSSLIN J. M.; UPTON J. E. 2007. Association of Bactericera cockerelli (Homoptera: Psyllidae) with “zebra chip”, a new potato disease in southwestern United States and Mexico. Journal of Economic Entomology 100 (3): 656-663. https://doi.org/10.1093/jee/100.3.656

MUSTAFA, T.; HORTON, D. R.; COOPER. W. R.; SWISHER, K. D.; ZACK, R. S.; MUNYANEZA, J. E. 2015. Interhaplotype fertility and effects of host plant on reproductive traits of three haplotypes of Bactericera cockerelli (Hemiptera: Triozidae). Environmental Entomology. 44 (2): 300-308. https://doi.org/10.1093/ee/nvu029

NACHAPPA, P.; LEVY, J.; PIERSON, E.; TAMBORINDEGUY, C. 2011. Diversity of endosymbionts in the potato psyllid, Bactericera cockerelli (Triozidae), vector of zebra chip disease of potato. Current Microbiology 62 (5): 1510-20. https://doi.org/10.1007/s00284-011-9885-5

NATIONAL CENTER FOR BIOTECHNOLOGY INFORMATION (NCBI). 1988. Bethesda (MD): National Library of Medicine (EE. UU.) [Check Date: August 8, 2016]. https://www.ncbi.nlm.nih.gov/

RAVINDRAN, A.; LEVY, J.; PIERSON, E.; GROSS, D. C. 2011. Development of primers for improved PCR detection of the potato zebra chip pathogen, ‘Candidatus Liberibacter solanacearum’. Plant Disease 95 (12): 1542-1546. https://doi.org/10.1094/PDIS-05-11-0386

SECOR, G. A.; RIVERA, V. V.; ABAD, J. A.; LEE, I. M.; CLOVER, G.R.G.; LIEFTING, L. W.; LI, X.; De BOER, S. H. 2009. Association of ‘Candidatus Liberibacter solanacearum’ with zebra chip disease of potato established by graft and psyllid transmission, electron microscopy, and PCR. Plant Disease 93 (3): 574-583. https://doi.org/10.1094/PDIS-93-6-0574

SWISHER, K. D.; MUSTAFA, T.; COOPER, R. W.; MUNYANEZA, J. E. 2018. Role of ‘Candidatus Liberibacter solanacearum’ and Bactericera cockerelli Haplotypes in Zebra Chip incidence and symptom severity. American Journal of Potato Research 95: 709-719. https://doi.org/10.1007/s12230-018-9678-5

SWISHER, K. D.; HENNE, D. C.; CROSSLIN, J. M. 2014. Identification of a fourth haplotype of Bactericera cockerelli (Hemiptera: Triozidae) in the United States. Insect Science 14 (161): 1-7. https://doi.org/10.1093/jisesa/ieu023

SWISHER, K. D.; ARP, A. P.; BEXTINE, B. R.; AGUILAR, E. Y.; CROSSLIN, J. M.; MUNYANEZA, J. E. 2013b. Haplotyping the Potato Psyllid, Bactericera cockerelli, in Mexico and Central America. Southwestern Entomologist 38 (2): 201-208. http://dx.doi.org/10.3958/059.038.0205

SWISHER, K. D.; MUNYANEZA, J. E.; CROSSLIN, J. M. 2013a. Temporal analysis of potato psyllid haplotypes in the United States. Environmental Entomology 42 (2): 381-393. https://doi.org/10.1603/EN12261

SWISHER, K. D.; MUNYANEZA, J. E.; CROSSLIN, J. M. 2012. High resolution melting analysis of the Cytochrome Oxidase I gene identifies three haplotypes of the potato psyllid in the United States. Environmental Entomology 41 (4): 1019-1028. http://dx.doi.org/10.1603/EN12066

Received 2021-03-23
Accepted 2022-06-28
Published 2022-09-06