Production of a biopesticide based on Bacillus thuringiensis subsp. kurstaki, in the laboratory
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Abstract
In Colombia, the widespread utilization of bioinsecticides such as Bacillus thuringiensis (Bt) has to be supported with products that can compete both in price and availability with chemical pesticides. The objective of this work was to study different nutrients derived from agro-industrial sources, for the production of B. thuringiensis subsp. kurstaki (Btk) and to optimize the process in a 1-liter fermenter. With this purpose, biomass and spores production was followed-up spectrophotometrically and by plating in Luria Bertani medium (LB), respectively. Furthermore, the toxicity of the final product was evaluated on larvae ofSpodopterafrugiperda (Lep: Noctuidae) using a diet surface bioassay. The present work allowed the design of a culture medium that yields the following results in a 1-liter fermentation: Biomass 4,03 g/ 1, spore concentration 21„107 sp/ml, potency 1.284.002 Ul/ml, price US$0,03/1. These results are highly promising, because the medium is economic, produces an acceptable quantity of spores and biomass and the latter exhibits high toxicity on lepidopterous larvae.
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