Detection of Beauvericin in the entomopathogenic fungus Beauveria bassiana by using polyclonal antibodies

https://doi.org/10.25100/socolen.v30i2.9540
BEA ELISA Mycotoxin

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Published: Jul 15, 2004
Issue: Vol. 30 No. 2 (2004)
Section Agriculture / Research paper

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Authors

JORGE W. ARBOLEDA-V. Centro Nacional de Investigaciones de Café (Cenicafé)
FERNANDO DELGADO-B Universidad Católica de Manizales
ARNUBIO VALENCIA-J. Universidad de Caldas
Abstract

The entomopathogenic fungus Beauveria bassiana (Bals.) Vuillemin is a good tool for biological control of insects that attack economically important crops. This fungus produces a ciclohexadepsipeptide toxin that exhibits biological activity as an insecticide. The objective of this research was to produce polyclonal antibodies (Pabs) that could be used for the immunological quantification method in order to quantify of the production of beauvericin in seven (7) strains of B. bassiana (Bb) from the collection of Cenicafe-Colombia. Liquid cultures of Sabouraud "s medium were inoculated with 3,0 x 105 conidia/ml of each isolate and these extracts were filtered and evaluated at 7, 9 and 11 days of growth. Pabs were obtained through two intramuscular injections in New Zealand white rabbits. The injections contained 2,5 mg of the emulsified immunogen with f'reund's complete adjuvant for the first irtjection and with Freund's incomplete adjuvant for the second. The serological tests used in this experiment were ELISA and Dot-blot. The results showed differences in toxin production among seven isolates of the fungus B. bassiana. Ali isolations showed a peak of production between 9 and 1 1  days of culture. The levels of the beauvericin detected fluctuated between L 16 µ.g/¡,I for isolate B. bassiana 9610 and 1,57 µg / µl for isolate B. bassiana 9205. This methodology will permit selection of the strains most efficient in toxin production, which could be incorporated into integrated pest management programs.

How to Cite
ARBOLEDA-V., J. W., DELGADO-B, F., & VALENCIA-J., A. (2004). Detection of Beauvericin in the entomopathogenic fungus Beauveria bassiana by using polyclonal antibodies. Revista Colombiana De Entomología, 30(2), 125–130. https://doi.org/10.25100/socolen.v30i2.9540
References

ABBAS A. K.; LlCHTMAN A., H.; POBER, J. S. 1995. Inmunología Celular y Molecular. Interamericana Mcgraw-Hill. Madrid España. 517 p.

BALL, E. 1974. Serological test for the identification of plant viruses. Phytopathology. NewYork 3 I p.

BUCHAWALDT L., L.; JENSEN, J. S. 1991. HPLC purification of destruxins produced by Alternaría brassicae in culture and leaves of Brassica napus. Biochemistry 30 (7): 2311-2316.

BUSTILLO P., A. E.; CASTILLO H., A.; VILLALBA O., D. A.; VÉLEZ A., P.E.; MORALES O., E. 1991. Evaluaciones de campo con el hongo Beauveria bassiana para el control de la broca del café Hypothenemus hampei en Colombia. p. 679-686. En: COLLOQUE Scientifique International sur le café, 14. San Francisco, juillet 14-19,1991 París, ASIC.

BUSTlLLO P., A. E.; CÁRDENAS, M.R.; VILLALBA G., D. A.; BENAVIDES M., P.; OROZCO H., J.; POSADA F., F.J. 1998. Manejo Integrado de la Broca del Café Hypothenemus hampei (Ferrari) en Colombia. Centro Nacional de Investigaciones en Café CENICAFE.Chinchiná, Colombia. 134 p.

CASTELLANOS, D. 1997. Importancia en la patogenicidad de la acción enzimática el hongo Beauveria bassiana sobre la broca del café. Revista Colombiana de Entomología 23 (1-2): 65-71.

CHAMPLlN F., R.; GRULA E. A. 1979. Noninvolvement of Beauvericin in the entomopathogenicity of Beauveria bassiana. Applied and Environmental Microbiology 37: 1122-1125.

DELGADO, F.; LÓPEZ, Y.; GIRADO E, M. 2001. Actividad enzimática de hongos y su patogenicidad sobre Hypothenemus hampei. Manejo Integrado de Plagas. Costa Rica. Junio, N° 60 p. 43-49 .

DEV, S.; KOUL, O. 1997 Insecticides of natural origino Amsterdam, Mardwood Academic Publisher. 365 p.

DI PAOLA, R.; NENNA, S.; FORNELLY, F.; MORETTI, A.; LOGRIECO, A.; CAIAFFA, M. F.; BOTTALlCO, A.; TURSI, A.; MACCHIA,L. 1994. Cytotoxicity of Beauvericin on humans B-Iymphocyte cell lines. in ICACI XV EMCI 94. Allergy and Clinical Immunology News 2, p. 256.

ESTRADA V., M. N.; VÉLEZ A., P.E.; MONTOYA R., E. C.. BUSTlLLO P., A. E. 1999. Esporulación, germinación y patogenicidad de aislamientos monoespóricos de Beauveria bassiana. Cenicafé 50 (1): 49-65.

EVLAKHOVA, A.; RAKITIN, A. 1968. Insecticidas biológicos y sus aplicaciones. Doklady Academic Nauk. SSSR 178: 485-488.

EYAL, J.; MABUD,K. J.; FISCHBEIN,J. F.;WALTER, J. F.; OSBORNE,L. S. 1994. Assessment of Beauveria bassiana Eco-lstrain, which produces a red pigments for microbiological control. Applied Biochemistry and Biotechnology 44: 65-79.

FERRON, P. 1981. Pest control by the fungi Beauveria bassiana and Metarhizium anisopliae. En: BURGES,M.(ed. ) Microbial control of pest and plant diseases. 19701980. London, Academic Press. p. 465482.

GENTHNER, F.J.; MIDDAUGH, D. P.1994. Effects of Beauveria bassiana and its toxins on Mysidopsis bahia (Mysidaceae). Archives of Environmental Contamination and Toxicology 26: 90-94.

GONZÁLEZ G., M.T.; POSADA F., F.J.; BUSTlLLO> P.,A. E. 1993. Desarrollo de un bioensayo para evaluar la patogenicidad de Beauveria bassiana sobre Hypothenemus hampei Cenicafé 44 (3): 93-102.

GUPTA, S.; MONTLOR, C. HUANG, Y. S. 1995. Isolation of novel beauvericin analogues from the fungus Beauveria bassiana. Journal of Natural Products 58 (5): 733738.

IGNOFFO, C. M.; MANDAVA, N. B. 1988. Handbook of natural pesticides. V. 5 Microbial Insecticides. Part A. Entomogenous protozoa and fungí. Boca Ratón, CRCPress. 243 p.

JEGOROV, A.; SEDMERA, P.; MAVLICEK, V.; MATMA, V. 1998. Destruxin Ed1 a cyclopeptide the fungus Metarhizium anisopliae. Phytochemistry 49 (6): 1815-1817.

KERSHAW M, .J.; MOORHOUSE E, .R.; BATEMAN, R.; REYNOLDS, S. E.; CHARNLEY,A. K. 1999. The role of destruxins in the pathogenicity of Metarhizium anisopliae for three species of insect. Journal of Invertebrate Pathology 74: 2 ¡j-223.

KUCERA, M. 1971 Toxins of the entomophagous fungus Beauveria bassiana. 11. Effect of nitrogen sources on formation of the toxic protease in submerged culture. Journal of Invertebrate Pathology 17: 211-215.

MOCHIZUKI, K.; OHMORI, K.; TAMURA, M.; SHIZURI, Y.; NISHIYAMA, S.; MIYOSHI, E.; YAMAMURA,S. 1993. The structures of bioactive cyclodepsipeptides, beauveriollides I and II,metabolites of entomopathogenic fungi Beauveria sp. Bulletin of lhe chemical Sociely of Japan 66: 30413046.

NAMATAM, E.; TOMODA, H; TABATA, N.; SI. S.Y.; OMURA, S. 1999. Structure elucidation of fungal Beauveriolide-III, a novel inhibitor of lipid droplet formation in mouse macrophages. The Journal of Antibiotic 52: 7-12.

NILANONTA,C. H.; ISAKA, M.; KITTAKOOP, P.; PALlTTAPONGARNPIM P; KAMCHONWONGPAlSAN, S.; PITTAYAKHAJONWUT D.,;TANTICHAROEN, M.; THEBTARANONTH Y,. 2000. Antimycobacterial and Antiplasmodial Cydodepsipeptides from the Insect Pathogenic Fungus Paecilomyces tenuipes BCC 1614. Planta Médica 66: 756-758.

OJCIOUS D., M.; ZYCHLlNSKY, L.; ZHENG, M.; YONG, D. E. 1999. lonophore-induced apoptosis: role of DNA fragmentation and calcium fluxes. Experimental Cell Research 197: 43-49.

PATERSON, l.; CHARNLEY, K.; COOPER, R.; CLARKSON, J. 1994. Partial characterizations of specific induce of a cuticle degrading protease from the insect pathogenic fungus Metarhizium anisopliae. Microbiology (140): 3153-3159.

ROMBACH M., C. 1988. Production of Beauveria bassiana (Deuteromycotina: Nyphomycetes) sympoduloconidia in submerged culture. Entomophaga 33: 315-324.

SIGMA.2001. Catalog Sigma Aldrich 2000 2001l. USA.2843 p.

SMITH, R. J.; PEKRUL, S.; GRULA, E. A. 1981. Requirement for sequential enzymatic activities for penetration ofthe integument of the corn earworn (Heliothis zea). Journal of lnvertebrate Pathology 38: 335-344.

STRASSER N, ABENDSTEIND, STUPPNERN, BUTT, T. 2000. Monitoring the distribution of secondary metabolites produced by the entomopathogenous fungus Beauveria brongniartii with particular reference to oosporein. Mycological Research 10: 12271233.

THAKUR, R.; SMITH, J. 1997. Liquid Chromatography I Thermospray I Mass Spectrometry Analysis of beauvericin. Journal of Agricultural Food Chemistry 45: 12341239.

URIBE, S. S.; SALDARRIAGA, Y.; PINEDA, F.; ARANGO, J.; VELEZ, I. 1997. Producción de beauvericina por Beauveria bassiana 940 I aislada sobre Lutzomya sp. (Diptera: Psychodidae) vectores de Leishmaniosis. Revista Colombiana de Entomología 23 (3-4): 137-143.

VÉLEZ A., P.E.; POSADA F., F.J.; MARíN M., P.; GONZÁLES, M. T.; OSORlOV, E.; BUSTILLO P. A. E. 1997. Técnicas para el control de calidad de formulaciones de hongos entomopatógenos. Chinchiná, CENICAFE, (Boletín Técnico Cenicafé N° 17) p. 1-34.

VÉLEZ A., P.E.; GONZÁLEZ, M. T.; RIVERA, A.; BERNAL M.G.; BUSTILLO P.,A. E.;ESTRADA, M. N. 1998. Caracterización de aislamientos de Beauveria bassiana y Metarhizium anisopliae de la colección de hongos entomopatógenos de CENICAFE.Resúmenes, XXV Congreso Sociedad Colombiana de Entomología. p. 89. Cali.

VEY, A.; QUIOT, J. M.; VAGO, C.: FARGES, J. 1985. Effect inmunodepresseur de toxines fongines: inhibition de la reaction de encapsulement multicéllulaire par les destruxines. Comtes Rendusde l'academie des Séauces 300: 647-651.

VEY, A.; NOAGLAND, R.; BUTT,T.M. 200 l. Toxic metabolites of fungal biocontrol agents, in Fungal biocontrol Agents: Progress, Problems &: Potential, (Bult, T.M.,Jackson, C. &: Magan, N., eds.) CABI, Wallingford.

VOLCY, C.: PARDO, V. M. 1994. Principios de micología. Universidad Nacional de Colombia. Medellín. 141 p.

WILSON D., M.; SYDENHAME., W; LOMBAERT, G. A.; TRUCKSESS M., W.; ABRAMSON, D. BRENNET G., A. 1998. Mycotoxin analytical techniques. p. 135-182. En: Mycotoxins in Agriculture and Food Safety (SINHA, K. K. and BHATNAGAR, D., eds.). Marcel Dekker, NewYork.

ZACNARUK, R. 1973. Penetrationof the cuticular layers of elaterid larvae (Coleoptera) by the fungus Metarhizium anisopliae and notes on bacterial invasion. Journal of lnvertebrate Pathology 21: 101-106.

ZIZCA, J.; WElSER, J. 1993. Effectof Beauvericin a toxic metabolite of Beauveria bassiana on the ultrastructure of Culex pipiens autogenicus larvae. Cytobios 75: 13-19.

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