Susceptibility of fifth instar nymphs of Rhodnius prolixus (Hemiptera: Reduviidae) to the action of the fungus Beauveria bassiana
Main Article Content
The effect of the entomopathogenic fungus Beauveria bassiana (strain UdeA13) was evaluatedon fifth instar nymphs of Rhodnius prolixus under laboratory conditions. The strain was isolated from R. pallescens in the region of San Onofre (Sucre) and cultivated on Sabouraud Dextrosa Agar (SDA). Theinsect colonies were propagated and reared under controlled conditions of temperature (25-27ºC) and relative humidity (80%) in darkness for growth and reproduction. The insects were feed weekly for 1 h with hen´s blood (Gallus gallus). The treatments consisted of conidial suspensions of the entomopathogenic fungus B. bassiana at concentrations of 3x105, 1x107, 3x108 and 3x109 conidia/ml which were sprayed on the insects using the Pulverization Tower emulator system. Control insects were sprayed with an aqueous solution of Tween 80 (0,01%). Effects were compared among fungus treatments and the controls. Significative differences (P<0,01) were found among all treatments, regardless of whether the control group was included in the analysis. The concentrations of 3x108 and 3x109 conidia/ml produced 90 to 100% of mortality between 7 and 9 days after treatment. The results of this research indicate that B. bassiana (UdeA13) can be considered as a possible agent for the biological control of the vector R. prolixus.
ARROYAVE, E. 1995. Susceptibilidad de algunas especies de triatominos al hongo entomopatógeno Beauveria bassiana. Trabajo de grado. Instituto de Biología. Universidad de Antioquia. Medellín. p. 40.
BOTERO, D.; RESTREPO, M. 1998. Parasitosis humanas. 3ª edición. Corporación para Investigaciones Biológicas. Medellín. Colombia. p. 203-222.
BURGERJON, A. 1956. Pulvérisation et poudrage en laboratoire par des préparations pathogènes insectiicides Ann INRA Epiphyties 7: 675-684.
CALLE, J. 2000. Vers un contròle microbiologique des populations colombiennes de Triatominae, insectes vecteurs de la maladie de Chagas. Tesis presentada para obtener el título de Doctor de la Universidad de Paris V. Univ. De París V. –René Descartes-Facultad de Medicina Necker.
CIDEIM. 1994. Manual de entomología médica para investigadores de América Latina. Fundación Centro Internacional de Entrenamiento e Investigaciones Médicas. Centro colaborador de la OMS. Sección Enfermedad de Chagas. p.280.
DIAS, J. C. P.; SCHOFIELD, C. J. 1999. The evolution of Chagas Disease (American Trypanosomiasis) Control after 90 years of Carlos Chagas Discovery. Memories Instituto Oswaldo Cruz, Río de Janeiro. 94 Suppl. I: 103-121.
FARGUES, J.; LUZ, C. 1998. Effects of fluctuating moisture and temperature regimes on sporulation of Beauveria bassiana on cadavers of Rhodnius prolixus. Biocontrol Science and Technology 8: 323-334.
FARGUES, J.; LUZ C. 2000. Effects of fluctuating moisture and temperature regimes on the infection potential of Beauveria bassiana for Rhodnius prolixus. Journal of Invertebrate Pathology 75 (3): 202-211.
FERRON, P.; FARGUES, J.; RIBA, G. 1991. Fungias microbial insecticides against pests. Handbook of applied mycology. Vol 2 (Arora, D. K., Ajello. L. & Mujerki, E. G., eds.) Dekker, New York, p. 665-706.
GUHL, F.; NICHOLLS, S. 2001. Manual de procedimientos para el diagnóstico de la Enfermedad de Chagas. Universidad de los Andes, Instituto Nacional de Salud, OMS. Santa Fe de Bogotá, Colombia. 98 p.
GUTIÉRREZ, A. 2003. Reconocimiento de termitas y hongos entomomopatógenos en la Reforestadora San Sebastián de Buena Vista, Magdalena. Tesis de Maestría. Universidad Nacional de Colombia, Sede Medellín. 176 p.
LECUONA, R. E.; EDELSTEIN, J. D.; BERRETTA, M. F.; LA ROSSA, F. R.; ARCAS, J. A. 2001. Evaluation of Beauveria bassiana (Hyphomycetes) Strains as Potential Agents for Control of Triatoma infestans (Hemiptera: Reduviidae). Journal of Medical Entomology 38 (2): 172-179.
LEE, E. T. 1980. Statistical methods for survival data analysis. Estados Unidos. Lifetime Learning. 557 p.
LENT, H.; WYGODZINSKY, P. 1979. Revision of the Triatominae (Hemiptera: Reduviidae), and their significance as Vectors of Chagas Disease. Bulletin of the American Museum of Natural History 163 (3): 410411.
LUZ, C.; FARGUES, J. 1999. Dependence of the entomopathogenic fungus, Beauveria bassiana, on high humidity for infection of Rhodnius prolixus. Mycopathologia 146 (1): 33-41.
LUZ, C.; TIGANO M.; IONIZETE G.; CORDEIRO C.; ALJANABI S. 1998a. Selection of Beauveria bassiana and Metarhizium anisopliae isolates to control Triatoma infestans. Memories Instuto Oswaldo Cruz, Rio de Janeiro 93 (6): 839-846.
LUZ, C.; SILVA, Y. G.; CORDEIRO, C. M.; TIGANO, M. S. 1998b. Beauveria bassiana (Hyphomycetes) as a possible agent for biological control of Chagas disease vectors. Journal of Medical Entomology 35 (6): 977-979.
LUZ, C.; ROCHA, L. F.; HUMBER, R. A. 2003. Record of Evlachovaea sp. (Hyphomycetes) on Triatoma sórdida in the State of Goiás, Brazil, and Its Activity Against Triatoma Infestans (Reduviidae, Triatominae). Journal of Medical Entomology 40 (4): 451-454.
MINISTERIO DE SALUD COLOMBIA. 1998. Boletín informativo. Programa Nacional para la prevención de la enfermedad de Chagas. p 4.
MIWAKO, T.; EDMANB, J. D. 2001. Lack of manipulation of Rhodnius prolixus (Hemiptera: Reduviidae) vector competence by Trypanosoma cruzi. Journal of Medical Entomology 39 (1): 44-51.
MORENO, J. 1997. Estudios epidemiológicos sobre la enfermedad de Chagas en algunas regiones de Colombia. Memorias curso de Pos-grado: genética poblacional de triatominos aplicada al control vectorial de la enfermedad de Chagas, Editores CORCAS, Santa Fe de Bogotá. Colombia. 180 p.
OMS 2002. Control of Chagas Disease. Second report of the WHO Expert Committee. Who Technical Report Series. Geneva. p 105.
PINEDA, F.; SALDARRIAGA, Y.; GOMEZ, C. 2002. Susceptibilidad de Rhodnius ecuadoriensis de quinto estado de desarrollo a la acción de Beauveria bassiana. Revista Colombiana de Entomología 28 (1): 9-12.
PINEDA, F.; SALDARRIAGA, Y.; CALLE, J.; URIBE, S. 2004. Susceptibility of Rhodnius pallescens (Hemiptera: Reduviidae) of fifth instar nymph to the action of Beauveria spp.. Aceptado para su publicación en Entomotropica.
ROMAÑA, C. 1992. Recherches sur les potentialites des Hyphomycetes Entomopathogenes (Fungi Imperfecti) dans lutte microbiologique contre les Triatominae (Heteroptera). Facultte de Medecine. Universite Montpellier. 135 p.
ROMAÑA, C. A.; FARGUES, J. F. 1992. Relative susceptibility of different stages of Rhodnius prolixus to the entomopathogenic hyphomycete Beauveria bassiana. Memorias Instituto Oswaldo Cruz, Río de Janeiro 87 (3): 363-368.
SCHOFIELD C. J. 1997. Bases técnicas y económicas para el control a gran escala de la enfermedad de Chagas. Memorias curso de Pos-grado: genética poblacional de triatominos aplicada al control vectorial de la enfermedad de Chagas, Editores Corcas, Santa Fe de Bogotá. Colombia. p. 180.
SCHOFIELD, C. J. 2000. Challenges of Chagas disease vector control on Central America (Position Paper). WHO Pesticide Evaluation Scheme (WHOPES), Global collaboration for Development of Pesticides World Health Organization, Communication Disease control and eradication. p. 240.
TANADA, Y.; KAYA, H. K. 1993. Insect pathology. Academic Press, Inc. Harcourt Brace Javanovich. Printed Unites States of America. p. 359-361.
URIBE, S.; SALDARRIAGA, Y.; PINEDA, F.; ARANGO, G. J.; VELEZ, I. D. 1997. Producción de beauvericina por Beauveria bassiana 9401 aislado sobre Lutzomyia sp. (Diptera: Psychodidae) vectores de Leishmaniosis. Revista Colombiana de Entomología 23 (3-4): 137-141.
- ANA ISABEL GUTIÉRREZ, YAMILLÉ SALDARRIAGA-O., SANDRA URJBE-S., FABIO PINEDA-G., Fungi associated with termites and their nests in eucalyptus plantations , Revista Colombiana de Entomología: Vol. 30 No. 1 (2004)
- ANA ISABEL GUTIÉRREZ-G., YAMILLÉ SALDARRIAGA-O., Observation of the pathogenicity of Metarhizium anisopliae in Nasutitermes sp. soldiers , Revista Colombiana de Entomología: Vol. 30 No. 2 (2004)
- ANA ISABEL GUTIÉRREZ-G., YAMILLÉ SALDARRIAGA-O., SANDRA URIBE-S., MÓNICA P. ZULUAGA-A., FABIO PINEDA-G., Pathogenicity of Paecilomyces lilacinus and Metarhizium anisopliae on Microcerotermes sp. termites (Isoptera: Termitidae) , Revista Colombiana de Entomología: Vol. 31 No. 1 (2005)
- FABIO PINEDA-G., YAMILLÉ SALDARRIAGA-O., CAROLINA GÓMEZ-L., Susceptibility of Rhodnius ecuadoriensis (Hemiptera: Reduviidae) of fifth instar nymph to the action of Beauveria bassiana , Revista Colombiana de Entomología: Vol. 28 No. 1 (2002)
- YAMILLÉ SALDARRIAGA-O., CARMEN A. VÁSQUEZ P., JAIME DE JESÚS CALLE-O., Virulence of Beauveria bassiana and Triflumuron on Rhodnius prolixus and R. pallescens (Hemiptera: Reduviidae) , Revista Colombiana de Entomología: Vol. 31 No. 2 (2005)
Similar Articles
- Magdiel Torres-De la Cruz, José del Carmen Gerónimo-Torres, Carlos Fredy Ortiz-García, Victoria Ayala Escobar, Manuel Pérez-De la Cruz, Silvia Cappello-García, Fungi associated with Hypothenemus hampei (Coleoptera: Curculionidae: Scolytinae) in Tabasco, Mexico , Revista Colombiana de Entomología: Vol. 45 No. 2 (2019)
- ANA ISABEL GUTIÉRREZ-G., YAMILLÉ SALDARRIAGA-O., SANDRA URIBE-S., MÓNICA P. ZULUAGA-A., FABIO PINEDA-G., Pathogenicity of Paecilomyces lilacinus and Metarhizium anisopliae on Microcerotermes sp. termites (Isoptera: Termitidae) , Revista Colombiana de Entomología: Vol. 31 No. 1 (2005)
You may also start an advanced similarity search for this article.
Authors retain the copyright on their work and are responsible for the ideas expressed in them. Once a manuscript is approved for publication, authors are asked for a publication license for the term of legal protection, for all territories that allows the use, dissemination and disclosure of the same.