Molecular characterization of Bombyx mori (Lepidoptera: Bombycidae) strains by AFLP

https://doi.org/10.25100/socolen.v32i2.9394
Sericulture Population structure Molecular markers

Article Sidebar

PDF (Español)

Published: Jul 15, 2006
Issue: Vol. 32 No. 2 (2006)
Section Agriculture / Research paper

Publcation metrics

61




 


Main Article Content

Authors

DUVERNEY GAVIRIA-A. Universidad Tecnológica de Pereira
ENRIQUE AGUILAR-F. Universidad Tecnológica de Pereira
LILIANA RAMÍREZ-L. Centro de Desarrollo Tecnológico de la Sericultura
ÁLVARO ALEGRÍA-S. Universidad Tecnológica de Pereira
Abstract

Twenty-three silkworm Bombyx mori L. strains (twelve Japanese and eleven Chinese strains), belonging to the CDTS (Technological Development Center for Sericulture) silkworm germplasm bank, were analyzed using the Amplified Fragment Length Polymorphism (AFLP) technique. Four combinations of primers were used. The four assayed combinations produced 247 bands with an average polymorphism of 54,6%; which, when corrected using Lynch and Milligan’s criteria, resulted in 168 bands with a polymorphism of 90,4%. The dendrogram was built using the UPGMA algorithm for the dissimilarity data, revealing two very well defined groups that correspond to the Japanese and Chinese geographical races. The heterozigocity average was higher in the Japanese race lines, showing a greater dispersion in the UPGMA and the main components analysis. The population structure study indicate that although the two races are not very different from each other, there exists a component of greater variance between the established groups than within them, which shows a significant level of genetic differentiation.

How to Cite
GAVIRIA-A., D., AGUILAR-F., E., RAMÍREZ-L., L., & ALEGRÍA-S., ÁLVARO. (2006). Molecular characterization of Bombyx mori (Lepidoptera: Bombycidae) strains by AFLP. Revista Colombiana De Entomología, 32(2), 221–226. https://doi.org/10.25100/socolen.v32i2.9394
References

ABRAHAM, E. G.; NAGARAJU, J.; DATTA, R. K. 1992. Biochemical studies of amylases in the silkworm, Bombyx mori L.: Comparative analysis in the diapausing and nondiapausing strain. Insect Biochemical and Molecular Biology 22 (8): 687-873.

DAMODAR, K.; ABRAHAM, E. G.; NAGARAJU, J. 1999. Microsatellites in the silkworm, Bombyx mori: Abundance, polymorphism, and strain characterization. Genome 42: 1057-1065.

DOIRA, H.; FUJII, H.; KAWAGUCHI, Y.; KIHARA, H.; BANNO, Y. 1992. Genetic stock and mutation of Bombyx mori. Institute of Genetic Resources, Kyushu University, Japon.

EXCOFIER, L.; SMOUSE, P. E.; QUATTRO, J. M. 1992. Analysis of molecular variance inferred from metric distances among DNA haplotypes: Application to human mitochondrial DNA restriction data. Genetics 131: 479-491.

GOWER, J. C. 1966. Some distance properties of latent roots and vector methods used in multivariate analysis. Biometrika 53: 325338.

LYNCH, M.; MILLIGAN, B. G. 1994. Analysis of population genetic structure with RAPD markers. Molecular Ecology 3: 91-99.

MILLER, M. P. 1998. AMOVA-PREP 1.01. A program for the preparation of ANOVA imput files from dominant marker raw data. Computer software distributed by the author.

MILLER, M. P. 1999. Tools for population genetic analysis (TFPGA) 1.3: A Windows program for the analysis of allozymes and molecular population genetic data. Computer software distributed by the author.

NAGARAJU, J. 2000. Recent advances in molecular genetics of the silk moth, Bombyx mori. Current Science 78 (2): 151161.

NAGARAJU, J. 2002. Application of the genetic principles for improving silk production. Current Science 83 (4): 409414.

NAGARAJU, J.; SHARMA, A.; SETHURAMAN, B. N.; RAO, G. V.; SINGH, L. 1995. DNA fingerprinting in the silkworm Bombyx mori using banded krait minor satellite DNA-derived probe. Electrophoresis 16: 1639-1642.

NAGARAJU, J.; REDDY, K. D.; NAGARAJA, G. M.; SETHURAMAN B. N. 2001. Comparison of multilocus RFLPs and PCR-based markers systems for genetic analysis of the silkworm, Bombyx mori. Heredity 86: 588-597.

NEI, M. 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89: 583590.

NEI, M.; LI, W. H. 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. USA. 76: 5269-5269.

REDDY, K. D.; NAGARAJU, J.; ABRAHAN, E. G. 1999. Genetic characterization of the silkworm Bombyx mori by simple sequence repeat (SSR)-anchored PCR. Heredity 83: 681-687.

ROHLF, F. J. 1998. NTSYS-PC numerical taxonomy and multivariate analysis system, Version 2.01. Owner´s manual. Exeter publication, Setauket, NY, USA.

RYMOND, M.; ROUSSET, F. 1995. An exact test for population differentiation. Evolution 49: 1280-1283.

SHARMA, A.; NIPHADKAR M. P.; NAGARAJU, J. SINGH, L. 1990. DNA fingerprint variability within and among the silkworm Bombyx mori varieties and estimation of their genetic relatedness using BKM-derived probe. The Journal of Heredity 90 (2): 315-319.

SOKAL, R. R.; ROHLF, F. J. 1981. Biometry. 2nd ed. Freemen, NY. 832 p.

SUZUKI, Y.; GAGE, L. P.; BROWN, D. D. 1972. The genes for fibroin in Bombyx mori. Journal of Molecular Biology 70: 637-649.

YEH, F. C.; YANG, R. C.; BOYLE, T. B.; YE, Z. H.; MAO, J. X. 1997. POPGENE, the user-friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Centre, University of Alberta, Edmonton, Alta.

VOS, P.; HOGERS, R.; BLEEKER, M.; REIJANS, M.; VAN DEER LEE, T.; HORNES, M.; FRIJTERS, A.; POT, J.; PELEMEN, J.; KUIPER, M.; ZABEAU, M. 1995. AFLP: A new technique for DNA fingerprinting. Nucleic Acids Research 23: 4407-4414.

Authors retain the copyright on their work and are responsible for the ideas expressed in them. Once a manuscript is approved for publication, authors are asked for a publication license for the term of legal protection, for all territories that allows the use, dissemination and disclosure of the same.