Preservation and storage of butterfly DNA using filter paper

https://doi.org/10.25100/socolen.v33i2.9345
Polimerase Chain Reaction (PCR) ND4 Lepidoptera

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Published: Dec 31, 2007
Issue: Vol. 33 No. 2 (2007)
Section Agriculture / Scientific notes

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Authors

LUZ MIRYAM GÓMEZ-P. Universidad Nacional de Colombia, Sede Medellín. Facultad de Ciencias
SANDRA I. URIBE Universidad Nacional de Colombia, Sede Medellín. Facultad de Ciencias
Abstract

Two techniques for butterfly DNA storage were compared using Polymerase Chain Reaction (PCR) amplification of a fragment from the ND4 gene. Extracted DNA was put into microcentrifuge tubes and stored at -20°C; alternatively, they were put on filter paper and stored at ambient temperatures. Fragments of DNA amplified through PCR that came from filter paper exhibited high quality. Our results suggest storage on filter paper to be a simple, effective and inexpensive alternative to preserve extracted DNA.

How to Cite
GÓMEZ-P., L. M., & URIBE, S. I. (2007). Preservation and storage of butterfly DNA using filter paper. Revista Colombiana De Entomología, 33(2), 197–199. https://doi.org/10.25100/socolen.v33i2.9345
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