Analysis of mitochondrial DNA sequences (Cytb and ND1) in Lucilia eximia (Diptera: Calliphoridae)

https://doi.org/10.25100/socolen.v37i2.9089
Forensic Entomology Citochrome b Molecular markers

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Published: Jul 15, 2011
Issue: Vol. 37 No. 2 (2011)
Section Agriculture / Scientific notes

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PAULA ANDREA GIRALDO-H. Universidad Nacional de Colombia, sede Medellín
SANDRA INÉS URIBE-S. Universidad Nacional de Colombia, sede Medellín
ANDRÉS LÓPEZ-R. Universidad Nacional de Colombia, sede Medellín
Abstract

Lucilia   eximia   (Diptera:   Calliphoridae)   is   a   species   of   forensic   importance   for   its   association  with  decomposing  bodies  in  Antioquia  and  other  regions  of  Colombia.  The  implementation  of  methods to allow the rapid and accurate identification of this species is a matter important in forensic entomology. Currently, the mitochondrial DNA sequences are considered to complement classical taxonomy and are used in the characterization of morphologically similar or indistinguishable species as in the case of forensic importance flies. We evaluated the variability of a mitochondrial region (Cytb –RNAt-Ser-ND1) of 480 bp that was amplified by PCR, from L. eximia larvae on third stage from Medellin and two other localities. The sequence differences evaluated as nucleotidic differences and genetic distances permitted the separation of individuals from the species of interest when compared with morphologically similar and related species. Furthermore, using a N.J analysis (K2p) the haplotype groupings were related to the geographical origin of individuals from L. eximia.

How to Cite
GIRALDO-H., P. A., URIBE-S., S. I., & LÓPEZ-R., A. (2011). Analysis of mitochondrial DNA sequences (Cytb and ND1) in Lucilia eximia (Diptera: Calliphoridae). Revista Colombiana De Entomología, 37(2), 273–278. https://doi.org/10.25100/socolen.v37i2.9089
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