B biotype of Bemisia tabaci (Hemiptera: Aleyrodidae): crop pest of pepper in the Cauca Valley, Colombia
Main Article Content
To identify the distribution of Bemisia tabaci biotype B in pepper crops in the Cauca Valley, Colombia, adult and pupal samples were taken from thirty-three sites in 12 different locations visited. Initial differentiation between the species Bemisia tabaci and Trialeurodes vaporariorum was based on morphological characteristics of the fourth instar (pupae). Reconfirmation of species identity and identification of biotypes was done by means of random amplified polymorphic DNA (RAPD’s-PCR analysis). The dominant species was B. tabaci B biotype, which was recorded in 50% of the samples. The A biotype of B. tabaci was not detected and a mixture of biotype B and T. vaporariorum was found in 31.2% of the samples. Levels of insecticide resistance were studied in adults and first instar nymphs of the B biotype, across six sites of the department with the methodologies and diagnostic doses previously established by CIAT. In addition, line base data, LC50, LC90 and diagnostic doses for thioxiclam hydrogen oxalate on adults and pyriproxifen and spiromesifen on first instar nymphs were calculated using the leaf-dip methodology. Adult populations showed high levels of resistance to methamidophos, and some cases of intermediate resistance to cypermethrin, imidacloprid and thioxyclam hydrogen oxalate. The nymphs from locations with excessive use of insecticides have shown intermediate resistance to imidacloprid, buprofezin and diafentiuron. The implications of these results in the management of insect in pepper crops are discuss.
ANDERSON, P. 2005. Introduction. pp. 1-11. In: Anderson, P.; Morales, F. (Eds.). Whitefly and Whitefly-Borne Viruses in the Tropics: Building a Knowledge Base for Global Action. Centro Internacional de Agricultura Tropical CIAT. Cali. Colombia. 351 p.
APRD 2009. Arthropod Pesticide Resistance Database. Disponible en: http://www.pesticideresistance.org/search/12/0/38/0/. [Fecha revisión: 29 noviembre 2009].
BUSVINE, J. R. 1971. A critical review of the techniques for testing insecticides. Commonwealth Agricultural Bureau. The Commonwealth Institute of Entomology, 56 Queens Gate, London. 345 p.
CABALLERO, R. 1994. Clave de campo para inmaduros de moscas blancas en Centroamérica (Homoptera: Aleyrodidae). Escuela Agrícola Panamericana, Departamento de Protección Vegetal, Sección de Entomología. El Zamorano (Honduras). 4 p.
CAHILL, M.; GORMAN, K.; DAY, S.; DENHOLM, I. 1996. Baseline determination and detection of resistance to imidacloprid in Bemisia tabaci (Homoptera: Aleyrodidae). Bulletin of Entomological Research. 86: 343-349.
CARDONA, C.; RENDÓN, F.; GARCIA, J.; LÓPEZ-ÁVILA, A.; BUENO, J.; RAMÍREZ, J. 2001. Resistencia a insecticidas en Bemisia tabaci y Trialeurodes vaporariorum (Homoptera: Aleyrodidae) en Colombia y Ecuador. Revista Colombiana de Entomología 27 (1-2): 33-38.
CARDONA, C.; RENDÓN, F.; RODRÍGUEZ. I.; LÓPEZ-ÁVILA, A. 2005. Whiteflies as pests of annual crops in the tropical highlands of Latin America: Insecticide resistance in Colombia and Ecuador. pp. 285-293. In: Anderson, P.; Morales, F. (Eds.). Whitefly and Whitefly-Borne Viruses in the Tropics: Building a Knowledge Base for Global Action. Centro Internacional de Agricultura Tropical CIAT. Cali. Colombia. 351 p.
CIAT 2007. Annual Report Improved Beans for the Develomping World. Centro Internacional de Agricultura Tropical. Cali. p. 205-210.
CROWDER, D.; DENNEHY, T.; ELLERS-KIRK, C.; YAFUSO, C.; ELLSWORTH, P.; TABASNIK, B.; CARRIERE, Y. 2007. Field evaluation of resistance to pyriproxifen in Bemisia tabaci (B biotype). Journal of Economic Entomology 100 (5): 1650- 1656.
DARDÓN, D. E. 1992. Las moscas blancas en Guatemala. pp. 38-41. En: Hilje, L.; Arboleda, O. (Eds.). Las moscas blancas en América Central y el Caribe. Memorias del Taller Centroamericano y del Caribe sobre moscas blancas, Turrialba, Costa Rica.
DITTRICH, V.; UK, S.; ERNST, H. 1990. Chemical control and insecticide resistance of whiteflies. pp. 263-285. In: Gerling, D. (Ed.). Whiteflies: Their Bionomics, Pest Status and Management. Intercep Limited. Andover. UK. 348 p.
DONG, C.; ZHANG, Y.; BIN, C.; BAOYUN, X.; QINGJUN, W.; GUOREN, Z. 2005. Sequence analysis of mtDNA COI gene and molecular phylogeny of different geographical populations of Bemisia tabaci (Gennadius). Agricultural Sciences in China 4 (7): 533-541.
ERDOGAN, C.; GRAHAM, D.; OKTAY, M.; GORMAN, K.; DENHOLM, I. 2008. Insecticide resistance and biotype status of populations of the tobacco whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) from Turkey. Crop Protection 27: 600-605.
GAWEL, N.J.; BARTLETT, A.C. 1993. Characterization of differences between whiteflies using RAPD-PCR. Insect. Molecular Biology 2: 33-38.
GORMAN, K.; WREN, J.; DEVINE, G.; DENHOLM, I. 2003. Characterization of neonicotinoid resistance in Bemisia tabaci from Spain. pp. 783-788. En: British Crop Protection Council (Eds.). The BCPC International Congress: Crop Science and Technology Vol. 1 y 2. Glasgow. Escocia. 900 p.
HOROWITZ, A.; KONTSEDALOV, S.; ISHAAYA, I. 2004. Dynamics of resistance to the neonicotinoids acetamiprid and thiamethoxam in Bemisia tabaci (Homoptera: Aleyrodidae). Journal of Economic Entomology 97 (6): 2051-2056.
JARA, L. A. 1998. Caracterización de la incidencia y manejo de mosca blanca en el valle de Comayagua. Tesis de Grado, Departamento de Protección Vegetal, Escuela Agrícola Panamericana (EAP), El Zamorano, HN. 84 p.
KONTSECLALOV, S.; GOTTLIEB, Y.; ISHAAYA, I.; NAUEN, R.; HOROWITZ, R.; GHANIM, M. 2009. Toxicity of spiromesifen to the developmental stages of Bemisia tabaci biotype B. Pest Management Science 65 (1): 5-13.
LIU, T.X. 2004. Toxicity and efficacy of spiromesifen, a tetronic acid insecticide, against sweetpotato withefly (Homoptera: Aleyrodidae) on melons. Crop Protection 23: 505-513.
MA, D.; GORMAN, K.; DEVINE, K.; LUO, W.; DENHOLM, I. 2007. The biptype and insecticide resistance status of Bemisia tabaci (Hemiptera: Aleyrodidae) invading cropping systems in Xinjiang Uygur Autonomus Region, northwestern China. Crop Protection 26: 612-617.
MARTÍNEZ-CARRILLO, J.; SERVIN-VILLEGAS, R.; NAVACAMBEROS, U.; CORTEZ-MONDACA, E.; GARCÍA-HERNÁNDEZ, J. 2006. A five-year study of insecticide resistance in whitefly Bemisia argentifolii Bellows & Perring from the Yaque Valley, Mexico. Southwestern Entomologist 31 (4): 307-320.
MARTÍNEZ-CARRILLO, J. L.; BROWN, J. K. 2007. First Report of the Q Biotype of Bemisia tabaci in Southern Sonora, Mexico. Phytoparasitica 35 (3): 282-284.
MORALES, F. 2005. Whiteflies as vectors of viruses in legume and vegetable mixed cropping systems in the Tropical lowlands of Central America, Mexico and the Caribbean. pp. 173-203. En: Anderson, P.; Morales, F. (Eds.). Whitefly and Whitefly-Borne Viruses in the Tropics: Building a Knowledge Base for Global Action. Centro Internacional de Agricultura Tropical CIAT. Cali. Colombia. 351 p.
NAUEN, R.; SUTUMPF, N.; ELBERT, A. 2002. Toxicological and mechanistic studies on neonicotinoid cross resistance in Q-type Bemisia tabaci (Hemiptera: Aleyrodidae). Pest Management Science 58: 868-875.
NAUEN, R.; BRESCHNEIDER, T.; ELBERT, A.; FISCHER, R.; TIENANN, R. 2003. Spirodiclofen y spiromesifen. Pesticide Outlook 14 (6): 243-245.
PALUMBO, J.; HOROWITZ, A; PRABHAKER, N. 2001. Insecticidal control and resistance management for Bemisia tabaci. Crop Protection 20 (9): 739-765.
PERRING, T. M. 2001. The Bemisia tabaci complex. Crop Protection 20 (9): 725 735.
PLAPP, F. W.; JACKMAN, J. A.; CAMPANHOLA, C.; FRISBIE, R. E.; GRAVES, J. B.; LUTRELL, R. G.; KITTEN, W. F.; WALL, M. 1990. Monitoring and management of pyrethroid resistance in the tobacco budworm (Lepidoptera: Noctuidae) in Texas, Mississippi, Louisiana, Arkansas, and Oklahoma. Journal of Economic Entomology 33 (2): 335-341.
PRABHAKER, N.; COUDRIET, D.; MEYERDIRK, D. 1985. Insecticide resistance in the sweetpotato whitefly Bemisia tabaci (Homoptera: Aleyrodidae). Journal of Economic Entomology 78: 748-752.
PRABHAKER, N.; CASTLE, S.; HENNEBERRY, T. J.; TOSCANO, N. C. 2005. Assessment of cross-resistance potential to neonicotinoid insecticides in Bemisia tabaci (Hemiptera: Aleyrodidae). Bulletin of Entomological Research 95 (6): 535-543.
QUINTERO, C.; CARDONA, C.; RAMÍREZ, D.; JIMÉNEZ, N. 1998. Primer registro del biotipo B de Bemisia tabaci (Homoptera: Aleyrodidae) en Colombia. Revista Colombiana de Entomología 24 (1-2): 23-28.
QUINTERO, C.; RENDÓN, F.; GARCÍA, J.; CARDONA, C.; LÓPEZ-ÁVILA, A.; HERNÁNDEZ, P. 2001. Especies y biotipos de moscas blancas (Homoptera: Aleyrodidae) en cultivos semestrales de Colombia y Ecuador. Revista Colombiana de Entomología 27 (1-2): 27-31.
RODRÍGUEZ, I.; CARDONA, C. 2001. Problemática de Trialeurodes vaporariorum y Bemisia tabaci (Homoptera: Aleyrodidae) como plagas de cultivos semestrales en el Valle del Cauca. Revista Colombiana de Entomología 27 (1-2): 21-26.
RODRÍGUEZ, I.; DURÁN, I.; MORALES, H.; CARDONA, C. 2003a. Líneas base, dosis diagnóstico y medición periódica de resistencia a imidacloprid, spinosad y carbosulfan en poblaciones de adultos de Thrips palmi (Thysanoptera: Thripidae) en el Valle del Cauca, Colombia. Revista Colombiana de Entomología 29 (1): 29-33.
RODRÍGUEZ, I.; MORALES, H.; CARDONA, C. 2003b. Líneas base, dosis diagnóstico y medición periódica de resistencia a insecticidas en poblaciones de adultos e inmaduros de Trialeurodes vaporariorum (Homoptera: Aleyrodidae) en el Valle del Cauca, Colombia. Revista Colombiana de Entomología 29 (1): 21-27.
RODRÍGUEZ, I.; MORALES, H.; BUENO, J.; CARDONA, C. 2005. El biotipo B de Bemisia tabaci (Homoptera: Aleyrodidae) adquiere mayor importancia en el Valle del Cauca. Revista Colombiana de Entomología 31 (1): 21-28.
SAS Institute Inc. 2000. SAS User’s guide version 8.1. SAS Institute, Cary, North Carolina, USA.
SEDILES, A. 1998. La mosca blanca Bemisia tabaci (Gennadius) en Nicaragua: Historia y problemática. Informe Universidad Nacional Agraria (UNA). Nicaragua. 23 p.
- ISAURA RODRÍGUEZ, HÉCTOR MORALES, JUAN MIGUEL BUENO, CÉSAR CARDONA M., The B biotype of Bemisia tabaci (Homoptera: Aleyrodidae) becomes more important in the Cauca Valley , Revista Colombiana de Entomología: Vol. 31 No. 1 (2005)
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Authors retain the copyright on their work and are responsible for the ideas expressed in them. Once a manuscript is approved for publication, authors are asked for a publication license for the term of legal protection, for all territories that allows the use, dissemination and disclosure of the same.