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Carbonic anhydrase (CA) catalyzes the reversible conversion of CO2 to bicarbonate and participates in mechanisms of alkalinization in the intestine of mosquitoes. The toxicity of four CA inhibitors (CAI ): acetazolamide (AZ M), methazolamide (MZM), ethoxolamide (ETX) and dorzolamide (DZA ) were evaluated in larvae of Anopheles albimanus by monitoring mortality 24, 48 and 72 hours post application, at a concentration of 50 ug/ml diluted in dimethyl sulfoxide previously. All IA C reduced the population of larvae in variable proportions. ETX showed the highest toxicity, achieving more than 80% mortality after 24 hours and 98% after 72 hours of application. The CAI , AZ M, MZM and DZA showed less toxicity (<50% mortality). Our results indicate that the CAI , including ETX in particular, is a worthy candidate as an alternative for the control of An. albimanus, which is considered a primary vector of malaria in Colombia.

Rocha O., L., Torres G., C., Corena-McLeod, M., Vélez B., I. D., & Robledo R., S. M. (2013). Effect of carbonic anhydrase inhibitors on Anopheles albimanus larvae (Diptera: Culicidae). Revista Colombiana De Entomología, 39(2), 226–228. https://doi.org/10.25100/socolen.v39i2.8240

BLANCO CASTRO, S. D.; COLOMBI, E.; FLORES, L. N. CANALES, D. 2002. Aplicación del biolarvicida Bacillus sphaericus 2362 (Griselesf) para el control de la malaria en un área de salud de la República de Honduras. Revista Cubana de Medicina Tropical 54: 134-141.

CARMONA-FONSECA, J. 2003. La malaria en Colombia, Antioquia y las zonas de Urabá y Bajo Cauca. Parte 1. Iatreia 16: 299-317.

CARMONA-FONSE CA, J. 2004. La malaria en Colombia, Antioquia y las zonas de Urabá y Bajo Cauca. Parte 2. Iatreia 17: 34-53.

CARRILLO, M. P.; SUÁREZ , M. F.; MORALES , A.; ESPINAL, C. A. 1981. Colonización y mantenimiento de una cepa colombiana de Anopheles albimanus, Wiedemann, 1820 (Diptera: Culicidae). Biomédica 1: 64-66.

CORENA, M. P.; SERON, T. J; LEHMAN, H. K.; OCHRIETOR, J. D.; KOHN, A.; TU, C.; LINSER, P. J. 2002. Carbonic anhydrase in the midgut of larval Aedes aegypti: cloning, localization and inhibition. The Journal of Experimental Biology 205: 591-602.

CORENA, M. P.; FIEDLER, M. M.; VANEKERIS , L.; TU, C.; SILVERMAN, D. N.; LINSE R, P. J. 2004. Alkalization of larval mosquito midgut and the role of carbonic anhydrase in different species of mosquitoes. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 137 (3): 207-225.

CORENA, M.; VANEKERIS , L.; SALAZA R, M. I.; BOWERS, D.; FIEDLER, M. M.; SILVERMAN, D.; TU, C.; LINSER, P. J. 2005. Carbonic anhydrase in the adult mosquito midgut. The Journal of Experimental Biology 208: 3263-3273.

FISHER, S. Z.; TARIKU, I.; CASE , N. M.; TU, C.; SERON, T.; SILVERMAN, D. N.; LINSER, P. J.; MCKENNA, R. 2006. Expression, purification, kinetic, and structural characterization of an alpha-class carbonic anhydrase from Aedes aegypti (AaCA1). Biochimica et Biophysica Acta 1764 (8): 1413-1419.

FONSECA, I.; CÁRDENAS, R.; GÓMEZ, W.; SANTACOLOMA, L.; BROCHERO, H.; OCAMPÓ, C. B.; SALAZA R, M.; MCALLISTER, J.; BROGDON, W.; QUIÑONEZ, M. 2010. Dosis diagnósticas para vigilar la resistencia a insecticidas de los vectores de malaria en Colombia. Revista Colombiana de Entomología 36: 54-61.

GEORGHIO U, G. P.; TAYLOR, C. E. 1977. Genetic and biological influences in the evolution of insecticide resistance. Journal of Economic Entomology 70 (3): 319-23.

GONZÁLEZ, R.; PALACIO MOSQUERA, J. D. 2009. Evaluación de la susceptibilidad y persistencia de los insecticidas Temephos (Abate) y Temephos (Instarphos), con exposición de larvas de Aedes aegypti y Anopheles nuñeztovari (Diptera: Culicidae). Vectors and Pest Magazine 1: 23-26.

GUTIÉRREZ, L. A.; NARANJO, N.; JARAMILLO, L. M.; MUSKUS, C.; LUCKHART, S.; CONNJ. E.; CORREA , M. M. 2008. Natural infectivity of Anopheles species from the Pacific and Atlantic regions of Colombia. Acta Trópica 107 (2): 99-105.

HEMINGWAY, J.; RANSO N, H. 2000. Insecticide resistance in Insect vectors of human disease. Annual Review of Entomology 45: 371-391.

MAESTRE, R.; REY, G.; DE LAS SALAS, J.; VERGARA, S. C.; SA NTACOLOMA, L.; GOENAGA, S.; CARRASQUILLA, M. C. 2009. Susceptibilidad de Aedes aegypti (Diptera: Culicidae) a temefos en Atlántico-Colombia. Revista Colombiana de Entomología 35 (2): 202-205.

MONTOYA-LERMA, J.; SOLARTE, Y. A.; GIRALDO -CALDERÓN, G. I.; QUIÑONES, M. L.; RUIZ -LÓPEZ, F.; WILKERSON, R. C.; GONZALEZ, R. 2011. Malaria vector species in Colombia A Review. Memórias do Instituto Oswaldo Cruz 106 (suppl. I): 223-238.

NAUEN, R. 2007, Insecticide resistance in disease vectors of public health importance. Pest Managment Science 63 (7): 628-633.

OLANO, V.; CARRASQUILLA, G.; MÉNDEZ, F. 1997. Transmisión de la malaria urbana en Buenaventura, Colombia: aspectos entomológicos. Revista Panamericana de Salud Pública 1 (4): 287-294.

ORGANIZACION MUNDIAL DE LA SALUD (WORLD HEALTH ORGANIZATION) OMS. 2005. Guidelines for laboratory and field testing of mosquito larvicides. WHO/CDS / WHOPES /GCDPP/2005.3.

SINKA, M. E.; RUBIO -PALIS, Y.; MANGUIN, S.; PATIL, A. P.; TEMPERLEY, W. H.; GETHING, P. W.; VAN BOECKEL, T.; KABARIA , C. W.; HARBACH, R. E.; HAY, S. I. 2010.The dominant Anopheles vectors of human malaria in the Americas: occurrence data, distribution maps and bionomic précis. Parasites & Vectors 3: 1-34.

SMITH, K. E.; VANEKERIS , L. A.; LINSER, P. J. 2007.Cloning and characterization of AgCA9, a novel W-carbonic anhydrase from Anopheles gambiae Giles sensustricto (Diptera: Culicidae) larvae. Journal of Experimental Biology 210: 3919-3930.

ZIMMERMAN, R. H. 1992. Ecology of malaria vectors in the Americas and future directions. Memórias do Instituto Oswaldo Cruz 87 (suppl. III ): 371-383.

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