Evaluación de la patogenicidad de Metarhizium robertsii y Beauveria bassiana contra Chloridea virescens (Lepidoptera: Noctuidae)
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In Mexico, Chloridea virescens is a pest of chickpea, tomato and other vegetables causing significant economic losses. To control this pest, three different strains (M1, M2 and M3) of Metarhizium robertsii (Previously Metarhizium anisopliae) and three strains of Beauveria bassiana (B1, B3 and B9) were evaluated under laboratory conditions, based on radial growth parameters, and conidia production and germination. The pathogenicity of the best strains was assessed at concentration of 1×108 conidia/mL, against second and third instar larvae of C. virescens, at 28 ± 1 °C, 70 ± 5 % of relative humidity and a photoperiod of 12 hours light. The six strains caused larvae mortality of C. virescens, ranging from 57 % to 97 %, with statistically significant differences compared with the control (P < 0.02). The M1 strain of M. robertsii exhibited the highest radial growth of 4.79 ± 0.02 cm, a growth rate of 3.33 mm/day, and a production of 6×109 conidia/mL at 14 days, with 96 % of germination at 24 hours. In terms of pathogenicity M1 caused 97 % of total larvae mortality at 7 days with TL50 of 4 days. The M2 and M3 strains caused mortality of 83 % and 77 % with a TL50 of 4 days. Strains B1, B3 and B9 have mortalities of 63 %, 57 % and 70 % with a TL50 of 4,3 days. (M1) M. robertsii had the best performance across all evaluated parameters, and it is therefore recommended for field testing to control of C. virescens.
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- Adara Graciano-Obeso , Cipriano García Gutíerrez, María Nancy Herrera-Moreno, Hervey Rodríguez González, Juan Carlos Sainz-Hernández, Gabriela Lizbeth Flores Zamora, Medium supplemented with insect pupae powder to induce growth of Beauveria bassiana and Metarhizium robertsii and the pathogenicity of Metarhizium robertsii , Revista Colombiana de Entomología: Vol. 49 No. 1 (2023): Vol. 49 Núm. 1 (2023)
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Accepted 2024-11-05
Published 2025-04-04

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