Main Article Content

Authors

 In Mexico, Chloridea virescens is a pest of chickpea, tomato and other vegetables causing significant economic losses. To control this pest, three different strains (M1, M2 and M3) of Metarhizium robertsii (Previously Metarhizium anisopliae) and three strains of Beauveria bassiana (B1, B3 and B9) were evaluated under laboratory conditions, based on radial growth parameters, and conidia production and germination. The pathogenicity of the best strains was assessed at  concentration of 1×108 conidia/mL, against second and third instar larvae of C. virescens, at 28 ± 1 °C, 70 ± 5 % of relative humidity and a photoperiod of 12 hours light. The six strains caused larvae mortality of C. virescens, ranging from 57 % to 97 %, with statistically significant differences compared with the control (P < 0.02). The M1 strain of M. robertsii exhibited the highest radial growth of 4.79 ± 0.02 cm, a growth rate of 3.33 mm/day, and a production of 6×109 conidia/mL at 14 days, with 96 % of germination at 24 hours. In terms of pathogenicity M1 caused 97 % of total larvae mortality at 7 days with TL50 of 4 days. The M2 and M3 strains caused mortality of 83 % and 77 % with a TL50 of 4 days. Strains B1, B3 and B9 have mortalities of 63 %, 57 % and 70 % with a TL50 of 4,3 days. (M1) M. robertsii had the best performance across all evaluated parameters, and it is therefore recommended for field testing to control of C. virescens.

Antonio Luna González , Instituto Politécnico Nacional

 
García Gutiérrez, C., Manzanarez-Jiménez, L. A., Rosas-García, N. M., & Luna González , A. (2025). Evaluación de la patogenicidad de Metarhizium robertsii y Beauveria bassiana contra Chloridea virescens (Lepidoptera: Noctuidae). Revista Colombiana De Entomología, 51(1). https://doi.org/10.25100/socolen.v51i1.13038

Abbott, W. S. (1925). A method of computing the effectiveness of an insecticide. Journal of Economic Entomology, 18(2), 265-267. https://doi.org/10.1093/jee/18.2.265a DOI: https://doi.org/10.1093/jee/18.2.265a

Acuña-Jiménez, M., Rosas-García, N., López-Meyer, M., Saínz-Hernández, J. C., Mundo-Ocampo, M., & García-Gutiérrez, C. (2015). Pathogenicity of microencapsulated insecticide from Beauveria bassiana and Metarhizium anisopliae against Tobacco budworm, Heliothis virescens (Fabricius). Southwestern Entomologist, 40(3), 531-538. https://doi.org/10.3958/059.040.0311 DOI: https://doi.org/10.3958/059.040.0311

Arévalo, M. H., & Zenner de Polanía, I. (2010). Evaluation of meridic diets suitable for efficient rearing of Heliothis virescens (F.) (Lepidoptera: Noctuidae). Revista U.D.C.A Actualidad & Divulgación Científica, 13(2), 163-173. https://doi.org/10.31910/rudca.v13.n2.2010.744 DOI: https://doi.org/10.31910/rudca.v13.n2.2010.744

Bugeme, D. M., Maniania, N. K., Knapp, M., & Boga, H. I. (2008). Effect of temperature on virulence of Beauveria bassiana and Metarhizium anisopliae isolates to Tetranychus evansi. Experimental Applied Acarology, 46(1-4), 275-285. https://doi 10.1007/s10493-008-9179-1 DOI: https://doi.org/10.1007/s10493-008-9179-1

Chandra Teja, K. N. P. & Rahman, S. J. (2016). Characterization and evaluation of Metarhizium anisopliae (Metsch.) Sorokin strains for their temperature tolerance. Micology, 7(4), 171-179. https://doi.org/10.3390/jof8010068 DOI: https://doi.org/10.1080/21501203.2016.1247116

Cheng, Y., Liu, T., Zhao, Y., Geng, W., & Liu, W. (2016). Evaluation of pathogenicity of the fungi Metarhizium anisopliae and Beauveria bassiana in Hazelnut weevil (Curculio nucum L., Coleoptera, Curculionidae) larvae. Indian Journal Microbiology, 56(4), 405-410. DOI: https://doi.org/10.1007/s12088-016-0614-4

http://doi10.1007/s12088-016-0614-4

Faria, M. R., & Wraight, S. P. (2007). Mycoinsecticides and mycoacaricides: a comprehensive list with worldwide coverage and international classification of formulation types. Biological Control, 43(3), 237-256. https://doi.org/10.1016/j.biocontrol.2007.08.001 DOI: https://doi.org/10.1016/j.biocontrol.2007.08.001

Finney, D. J. (1978). Statistical method in biological assay. 3ra edición. New York: Cambridge University Press, pp. 1-98. https://doi.org/10.1002/bimj.4710210714 DOI: https://doi.org/10.1002/bimj.4710210714

García-Gutiérrez, C., Cortez-Mondaca, E., & Manzanarez-Jiménez, L. A. (2019). Reseña del complejo Heliothis spp., y análisis de su situación actual en cultivos de importancia económica en Sinaloa. En Memoria del XLII Congreso Nacional de Control Biológico, Boca del Rio Veracruz, México. https://drive.google.com/file/d/1pXX8OmiN-okiq4TzPd-IMGErvO6j5jjA/view

Manzanarez-Jiménez, L. A., García-Gutiérrez, C., Flores-Zamora, G. L., Graciano-Obeso A., & Leal-Jiménez, L. M. (2021a). Desarrollo y hábitos de Chloridea virescens en tomate cultivado en traspatio. En Memorias del XLIII Congreso Nacional de Control Biológico, Chihuahua, México. https://drive.google.com/file/d/1O8yTmnqbPeYBKZdo-wcsdr8NTHsUmW21/view

Manzanarez-Jiménez, L. A., García-Gutiérrez, C., Rosas-García, N. M., Luna-González, A., & Escobedo-Bonilla, C. M. (2021b). Evaluación de formulaciones en aceites de Beauveria bassiana y Metarhizium anisopliae contra Chloridea virescens F. Southwestern Entomologist, 46(2), 503-510. https://doi.org/10.3958/059.046.0219 DOI: https://doi.org/10.3958/059.046.0219

Manzanarez-Jiménez, L. A., & García-Gutiérrez, C. (2020). Producción de esporas de Metarhizium anisopliae y Beauveria bassiana por cultivo bifásico y su patogenicidad contra Heliothis virescens F. En A. Merlo & S.C. Márquez (Eds.), Memorias del 4to Congreso nacional de investigación interdisciplinaria. Enfrentando retos emergentes de ciencia y tecnología (pp. 123-126). Ciudad de México, México SEPI-UPIICSA-IPN. https://www.scribd.com/document/492070594/4to-Congreso-Nacional-de-Investigacion-Interdisciplinaria-Enfrentando-retos-emergentes-de-Ciencia-y-Tecnologia

Minitab, L.L.C. (2021) Minitab. Recuperado de https:www.minitab.com

Inglis, G. D., Enkerli, J., & Goettel, M. S. (2012). Laboratory techniques used for

entomopathogenic fungi: Hypocreales. En L. Lacey (Ed), Manual of techniques in invertebrate pathology (pp. 189-253). Londres, Reino Unido: Academic Press. https://doi.org/10.1016/B978-0-12-386899-2.00007-5 DOI: https://doi.org/10.1016/B978-0-12-386899-2.00007-5

artínez-Valenzuela, C., G., Romano-Casas., Cuadras-Berrelleza, A. A., & Ortega-Martínez, L. D. (2019). Plaguicidas, impacto en salud y medio ambiente en Sinaloa (México): Implicaciones y retos en gobernanza ambiental. Trayectorias Humanas Trascontinentales, (4). https://doi.org/10.25965/trahs.1615 DOI: https://doi.org/10.25965/trahs.1615

SAS Institute. (2009). STAT/SAS User’s Guide, 9.0. ed SAS Institute Cary, NC ,1-67.

SAS/STAT 9.1 UG

SIAP. (2021). Tomate rojo. Ciudad de México. Disponible en

https://www.gob.mx/siap/acciones-y-programas/producción-agrícola-33119

Sun, J., Fuxa, J. R., & Henderson, G. (2002). Sporulation of Metarhizium anisopliae and Beauveria bassiana on Coptotermes formosanus and in vitro. Journal of Invertebrate Pathology, 81(2), 75-88. https://doi:10.1016/S0022-2011(02)00152-0 DOI: https://doi.org/10.1016/S0022-2011(02)00152-0

Vega, E. F., Luangsa-Ard, J. J., Meyling, N. V., & Blackwell, M. (2012). Fungal entomopathogens. En F. E. Vega & H.K. Kaya (Eds.), Insect Pathology (pp. 171-220), Academic Press. https://doi: 10.1016/B978-0-12-384984-7.00006-3 DOI: https://doi.org/10.1016/B978-0-12-384984-7.00006-3

Similar Articles

You may also start an advanced similarity search for this article.

Received 2023-06-30
Accepted 2024-11-05
Published 2025-04-04