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10.25100/socolen.v51i1.13038
Submitted: 30-06-2023
Published: 04-04-2025

Evaluación de la patogenicidad de Metarhizium robertsii y Beauveria bassiana contra Chloridea virescens (Lepidoptera: Noctuidae)

Instituto Politécnico Nacional
Instituto Politécnico Nacional
Instituto Politécnico Nacional
Instituto Politécnico Nacional
Author biography
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Antonio Luna González

 
Keywords: Entomopathogenic fungi, Metarhizium robertsii, radial growth, pathogenicity, strain

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García Gutiérrez C, Manzanarez-Jiménez LA, Rosas-García NM, Luna González A. Evaluación de la patogenicidad de Metarhizium robertsii y Beauveria bassiana contra Chloridea virescens (Lepidoptera: Noctuidae). Rev. Colomb. Entomol. [Internet]. 2025 Apr. 4 [cited 2026 Oct. 16];51(1). Available from: https://revistacolombianaentomologia.univalle.edu.co/index.php/SOCOLEN/article/view/13038
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Abstract

 In Mexico, Chloridea virescens is a pest of chickpea, tomato and other vegetables causing significant economic losses. To control this pest, three different strains (M1, M2 and M3) of Metarhizium robertsii (Previously Metarhizium anisopliae) and three strains of Beauveria bassiana (B1, B3 and B9) were evaluated under laboratory conditions, based on radial growth parameters, and conidia production and germination. The pathogenicity of the best strains was assessed at  concentration of 1×108 conidia/mL, against second and third instar larvae of C. virescens, at 28 ± 1 °C, 70 ± 5 % of relative humidity and a photoperiod of 12 hours light. The six strains caused larvae mortality of C. virescens, ranging from 57 % to 97 %, with statistically significant differences compared with the control (P < 0.02). The M1 strain of M. robertsii exhibited the highest radial growth of 4.79 ± 0.02 cm, a growth rate of 3.33 mm/day, and a production of 6×109 conidia/mL at 14 days, with 96 % of germination at 24 hours. In terms of pathogenicity M1 caused 97 % of total larvae mortality at 7 days with TL50 of 4 days. The M2 and M3 strains caused mortality of 83 % and 77 % with a TL50 of 4 days. Strains B1, B3 and B9 have mortalities of 63 %, 57 % and 70 % with a TL50 of 4,3 days. (M1) M. robertsii had the best performance across all evaluated parameters, and it is therefore recommended for field testing to control of C. virescens.

528 399

Antonio Luna González , Instituto Politécnico Nacional

 
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How to Cite

1.
García Gutiérrez C, Manzanarez-Jiménez LA, Rosas-García NM, Luna González A. Evaluación de la patogenicidad de Metarhizium robertsii y Beauveria bassiana contra Chloridea virescens (Lepidoptera: Noctuidae). Rev. Colomb. Entomol. [Internet]. 2025 Apr. 4 [cited 2026 Oct. 16];51(1). Available from: https://revistacolombianaentomologia.univalle.edu.co/index.php/SOCOLEN/article/view/13038
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Copyright (c) 2025 Cipriano García Gutiérrez, Lucía Araceli Manzanarez-Jiménez, Ninfa M, Rosas-García, Antonio Luna González