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 Among a series of exotic pests that have been reported in Chile, Xanthogaleruca luteola (Coleoptera: Chrysomelidae), a defoliating insect of elms (Ulmus spp.), is causing important damage in urban trees in central Chile. The insecticidal capacity of water and ethanol extracts obtained from new and mature leaves of Cestrum parqui (Solanaceae) was evaluated at various concentrations on X. luteola adults in the laboratory. Bioassays with a completely randomized design were used to analyze the effects of each extract. Also, the mean lethal concentration (LC50) was determined for both mature leaf states and solvents. A proximal analysis was done to determine the physical and chemical differences between the maturity stages. Proximal analysis of the powder of both leaf stages revealed differences (P < 0.01) in the content of humidity, ashes, lipids, and proteins, but not in the raw fiber content nor the non-nitrogenous extract. On the third day after exposure, the order of decreasing effectiveness (LC50) of
the insecticide extracts was: new-ethanol (2.5 % w/v), new-water (3.8 % w/v), mature-ethanol (6.7 % w/v) and mature-water (9.2 % w/v). Our results suggest that C. parqui leaf extracts may have potential use as a bioinsecticide in integrated pest management plans for the control of X. luteola adults, however, tests at a larger scale are necessary to confirm our results.

Huerta , A. ., Chiffelle, Ítalo, Araya, L., Curkovic, T., & Araya, J. (2021). Insecticidal capacity of Cestrum parqui (Solanaceae) leaf extracts on Xanthogaleruca luteola (Coleoptera: Chrysomelidae) adults. Revista Colombiana De Entomología, 47(2).

ASSOCIATION OF OFFICIAL ANALYTICAL CHEMISTS. 1984. Official methods of analysis. 14th Edition. AOAC. Virginia, EE.UU. 1141 p.

BAQAI, F. T.; ALI, A.; AHMAD, V. U. 2001. Two new spirostanol glycosides from Cestrum parqui. Helvetica Chimica Acta 84 (11): 3350-3356. 84:11<3350::AID-HLCA3350>3.0.CO;2-U

BARBOUCHE, N.; AMMAR, M.; HAJJEM, B.; LOGNAY, G. 2001. Contribution to the study of the biological activity of Cestrum parqui L’Hérit. extracts against the locust Schistocerca gre-

garia. Biotechnology, Agronomy, Society Environment 5 (2): 85-90.

BOROWIEC, L.; SEKERKA, L. 2010. Cassidinae. pp. 368-390. In: Löbl, I.; Smetana, A. (Eds.). Catalogue of Palaearctic Coleoptera. Vol 6. Apollo Books. Wroclaw, Polonia. 924 p.

CHAIEB, I.; HALIMA-KAMEL, M. B.; HAMOUDA, M. H. B. 2001. Effects of diet addition of Cestrum parquii (Solanaceae) extracts on some Lepidoptera pests: Pieris brassicae (Pieridae) et Spodoptera littoralis Boisduval (Noctuidae). Medlinden Faculteit Landbouww Universiteit Gent 66: 479-480.

CHAIEB, I.; HALIMA-KAMEL, M. B.; HAMOUDA, M. H. B. 2006. Insect growth regulator activity of Cestrum parqui saponins: an interaction with cholesterol metabolism. Communica-

tions in Agricultural and Applied Biological Sciences 71 (2): 489-496.

CHAIEB, I.; BOUKAMCHA, H.; BEN JANNET, H.; BENHALIMA M.; BEN HAMOUDA, M. H.; MIGHRI, Z. 2007. Purification of a natural insecticidal substance from Cestrum parqui (Solanaceae). Pakistan Journal of Biological Sciences 10 (21): 3822-3828.

CHAIEB, I.; HAMOUDA, A. B.; TRABELSI, M.; HALIMA, M. B.; HAMOUDA, M. H. B. 2009. Toxicity Investigation of Cestrum parqui Saponins to Culex pipiens Larvae. Pest Technology 3 (1): 73-75.

CHIFFELLE G.; I.; HUERTA F., A.; LIZANA R.; D. 2009. Physical and chemical characterization of Melia azedarach L. fruit and leaf for use as botanical insecticide. Chilean Journal of Agri-

cultural Research 69 (1): 38-45.

CHIFFELLE, I.; HUERTA, A.; AZÚA, F.; PUGA, K.; ARAYA, J. E. 2011a. Antifeeding and insecticide properties of aqueous and ethanolic fruit extracts from Melia azedarach L. on the elm

leaf beetle Xanthogaleruca luteola Müller. Chilean Journal of Agricultural Research 71 (2): 218-225.

CHIFFELLE, I.; HUERTA, A.; JIMÉNEZ, R.; ARAYA, J. E. 2011b. Proximal analysis and toxicity of extracts from young and mature leaves of boldo tree (Peumus boldus) on elm leaf beetle (Xanthogaleruca luteola). Canadian Journal of Forest Research 41 (12): 2259-2266.

CHIFFELLE, I.; HUERTA, A.; CELIS, M.; ARAYA, J. E. 2013. Proximal analysis and insecticidal effects of extracts from pepper tree (Schinus molle) leaves on elm leaf beetle (Xanthoga-

leruca luteola) larvae. Industrial Crops and Production 43: 523-528.

CHIFFELLE, I.; HUERTA, A.; BOBADILLA, V.; MACUADA, G.; ARAYA, J. E.; CURKOVIC, T.; CEBALLOS, R. 2019. Antifeedant and insecticidal effects of extracts from Melia azedarach fruits and Peumus boldus leaves on Xanthogaleruca luteola larvae. Chilean Journal of Agricultural Research 79 (4): 609-615.

DEFAGÓ, M.; VALLADARES, G.; BANCHIO, E.; CARPINELLA, C.; PALACIOS, S. 2006. Insecticide and antifeedant activity of different plant parts of Melia azedarach on Xanthogaleruca luteola. Fitoterapia 77 (7-8): 500-505.

DREISTADT, S. H.; DAHLSTEN, D. L.; LAWSON, A. B. 2014. Elm leaf beetle. UC Statewide IPM Program, University of California. Pest Notes Publication 7403: 1-5.

FOLCIA, A. M.; MICHETTI, M.; PELICANO, A. 2005. Aspectos biológicos y preferencia alimentaria de la vaquita del olmo Xanthogaleruca luteola (Müller) (Coleoptera: Chrysomelidae).

IDESIA (Chile) 23 (3): 7-12.

GROS, E. G.; POMILIO, A. B.; SELDES, A.; BURTON, G. 1985. Introducción al estudio de productos naturales. Organización de los Estados Americanos. Washington, EE. UU. 146 p.

GUCLU-USTUNDAG, O.; MAZZA, G. 2007. Saponins: properties, applications and processing. Critical Reviews in Food Science and Nutrition 47 (3): 231-258.

HOFFMANN, A. E. 1982. Flora silvestre de Chile: Zona Austral: una guía ilustrada para la identificación de las especies de plantas leñosas del sur de Chile: (árboles, arbustos y enredaderas leñosas). Ediciones Fundación Claudio Gay. Santiago de Chile, Chile. 258 p.

HUANQUILEF, C.; ESPINOZA, J.; MUTIS, A.; BARDEHLE, L.; HORMAZÁBAL, E; URZÚA, A.; QUIROZ, A. 2020. Antifeedant activities of organic fractions from Cestrum parqui leaves on

the Red-Haired Bark Beetle Hylurgus ligniperda. Journal of Soil Science and Plant Nutrition.

HUERTA, A.; CHIFFELLE, I.; PUGA, K.; AZÚA, F.; ARAYA, J. E. 2010. Toxicity and repellence of aqueous and ethanolic extracts from Schinus molle on elm leaf beetle Xanthogaleruca

luteola. Crop Protection 29 (10): 1118-1123.

HUERTA, A.; CHIFFELLE, I.; PUGA, K.; AZÚA, F.; JIMÉNEZ, R.; ARAYA, J. E. 2011. Life cycle of Xanthogaleruca luteola (Coleoptera: Chrysomelidae) in Santiago, Chile, and sex fenotype differentiation of adults. Boletín de Sanidad Vegetal. Plagas 37: 57-64.

INFOSTAT. 2009. InfoStat versión 2009. Grupo InfoStat, FCA, Universidad Nacional de Córdoba. Córdoba, Argentina. 334 p.

ISMAN, M. B. 2006. Botanical insecticides, deterrents, and repellents in modern agriculture and an increasingly regulated world. Annual Review of Entomology 51: 45-66. https://doi.


KUMAR, S. 2012. Biopesticides: a need for food and environmental safety. Journal of Biofertilizers & Biopesticides 3 (4): 1-3.

LAWSON, A. B.; DAHLSTEN, D. L. 2003. Evaluation of systemic insecticides as a treatment option in integrated pest management of the elm leaf beetle Xanthogaleruca luteola (Müller) (Coleoptera: Chrysomelidae). Journal of Economic Entomology 96 (5): 1455-1462.

LEFOE, G.; DOMINIAK, B.; WORSLEY, P.; DAVIES, J. 2014. Elm leaf beetle Xanthogaleruca luteola (Muller) dispersal across south eastern Australia (1989-2011). Plant Protection Quarterly

(2): 61-65.

MAISTRELLO, L.; LÓPEZ, M. A.; SORIA, F. J.; OCETE, R. 2005. Growth inhibitory activity of Daphne gnidium L. (Thymelaeaceae) extracts on the elm leaf beetle (Col., Chrysomelidae).

Journal of Applied Entomology 129 (8): 418-424.

NGO, T.; WROLSTAND, R. E.; ZHAO, Y. 2007. Color quality of Oregon strawberries-impact of genotype, composition, and processing. Journal of Food Science 72 (1): 25-32.

PAVELA, R. 2011. Antifeedant and larvicidal effects of some phenolic components of essential oils lasp lines of introduction against Spodoptera littoralis (Boisd.). Journal of Essential Oil

Bearing Plants 14 (3): 266-273.

RAFAEL, B.; MAREGGIANI, G.; FRASCHINA, A.; BILOTTI, G. 2000. Determinación de la actividad insecticida de Solanum sisymbriifolium, Cestrum parqui y Chenopodium album sobre adultos de Tribolium castaneum (Coleoptera: Tenebrionidae). Revista de la Facultad de Agronomía 20 (3): 373-377.

REGNAULT-ROGER, C.; VINCENT, C.; ARNASON, J. T. 2012. Essential oils in insect control: low-risk products in a high-stakes world. Annual Review of Entomology 57: 405-424.

ROBERTSON, J. L.; SMITH, K. C.; SAVIN, N. E.; LAVIGNE, R. J. 1984. Effects of dose selection and sample size on the precision of lethal dose estimates in dose-mortality regression.

Journal of Economic Entomology 77 (4): 833-837.

RODRIGO, E.; SANTACRUZ BOSCH, A. M.; XAMANÍ, P.; LABORDA, R. 2019. Life cycle, parasitism and damage of Xanthogaleruca luteola (Muller) in Valencia (SE Spain): a preliminary study. Urban Forestry & Urban Greening 46: 1-7.

ROMANYK, N.; CADAHIA, D. 2002. Plagas de insectos en las masas forestales. Mundi-Prensa. Madrid, Spain. 336 p.

SEGURA, R.; VÁSQUEZ, G.; COLSON, E.; GERBAUX, P.; FRISCHMON, C.; NESIC, A.; GARCÍA, D. E.; CABRERA-BARJAS, G. 2020. Phytostimulant properties of highly stable silver nanoparticles obtained with saponin extract from Chenopodium quinoa. Journal of the Science of Food and Agriculture. 100 (13): 4987-4994.

SHEKARI, M.; SENDI, J. J.; ETEBARI, K.; ZIBAEE, A.; SHADPARVAR, A. 2008. Effects of Artemisia annua L. (Asteraceae) on nutritional physiology and enzyme activities of elm leaf bee-

tle, Xanthogaleruca luteola Mull. (Coleoptera: Chrysomellidae). Pesticide Biochemistry and Physiology 91 (1): 66-74.

SUTHISUT, D.; FIELDS, P. G.; CHANDRAPATYA, A. 2011. Contact toxicity, feeding reduction, and repellency of essential oils from three plants from the ginger family (Zingiberaceae) and their major components against Sitophilus zeamais and Tribolium castaneum. Journal of Economic Entomology 104 (4): 1445-1454.

TAVARES, W. S.; CRUZ, I.; PETACCI, F.; ASSIS JÚNIOR, S. L.; SOUSA FREITAS, S.; ZANUNCIO, J. C.; SERRÃO, J. E. 2009. Potential use of Asteraceae extracts to control Spodoptera

frugiperda (Lepidop- tera: Noctuidae) and selectivity to their parasitoids Trichogramma praetiosum (Hymenoptera: Trichogrammatidae) and Telenomus remus (Hymenoptera: Scelionidae). Industrial Crops and Products 30 (3): 384-388.

ZAPATA, N.; BUDIA, F.; VIÑUELA, E.; MEDINA, P. 2006. Insecticidal effects of various concentrations of selected extractions of Cestrum parqui on adult and immature Ceratitis

capitata. Journal of Economic Entomology 99 (2): 359-365.