BIOCHEMICAL CHARACTERIZATION OF THE WHITE WORM OF THE POTATO Premnotrypes vorax BY MEANS OF ELECTROPHORETIC SEPARATION
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Abstract
The use of resistant varieties is one of the most economical ways of insect control. Before starting the process of breeding, it is necessary to know the insect variability in natural and artificial conditions. The conditions of electrophoretic separation on total proteins and esterases were established for the most important insect of the potato crop in Colombia, Premnostrypes vorax (Hustache). insect stages were analyzed, also gel conditions, electrode buffer and extraction media, in order to establish the genetic variability of the Andean weevil populations. The abdomen of the male. without the chitin, the cover was found to be the parí of the insect most adequate to work with. For the separation of esterases 20% sucrose should be used and 10% gel with alpha esterase and 15% with beta esterase; the buffer of the gel is 0.074M Tris - 0.009M Citric acid, ph = 8.4; the buffer of the electrodes is a stock solution of 0.036M LiOH and 0.194M Boric acid, pH= 8.25 diluted 1 :3 for the cathode and 1:4 for the anode.
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