Evaluation of the potencial of the entomogenous nematod Steinernema Carpocapsae Weiser (Rhabditida: Steinernematidae) for the control of Cyrtomenus bergi Froeschner (Hemiptera: Cydnidae) under laboratory condicionts
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The potential for the control of Cyrtomenus bergi by the entomopathogenic nematode, Steinernema carpocapsae in the laboratory was evaluated. Three methodologies, using all instars of C. bergi, were evaluated: a) Petri dishes (100x15mm) with filter paper and with unsterilized soil, with one insect per unit and one dose of 6.788.800 nematodes/ml. Results confirmed that C. bergi was infected by S. carpocapsae at 5 and 8 days after exposure; b) the second experiment used plastic cups (7x7x10cm) containing unsterilized soil in Petri dishes containing 10 insects of the same stage of development and doses of 2.000, 4.000, and 6.000 nematodes/ml. No clear tendency in parasitism was observed 2 and 8 days after exposure; c) the third experiment used plastic cups (3.Sx4x5cm) containing 10 g of sterile sand with 3% RH and one insect per cup, with 2.000, 4.000, 6.000, 8.000 and 10.000 nematodes/ml. Results showed that the adult stage is the most sensitive at all doses with 58.6% parasitism after 10 days; the least sensitive is the first and second instars with 17 and 31% parasitism, respectively. An additional experiment using the afore-described methodology evaluated the six nematode doses with adults. Probit analysis showed that an average dose of 192 nematodes/ml is required to obtain 50% parasitism, 10 days after inoculation. Plastic cups with sterile soil was the most successful experiment unit. Results showed that S. carpocapsae is a potential biological control agent of C. bergi.
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