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To detect and quantify the amount of Propoxur in insects of forensic importance and search for entomotoxicologic indicators, the HPLC (High Performance Liquid Chromatography) technique was used on individuals collected during the ecological succession associated with the corpses of rabbits.  Three rabbits were sacrificed with an overdose of Propoxur and a fourth control rabbit was sacrificed by cervical dislocation. The rabbits were checked over 28 days, three times a day (07:00, 13:00 and 18:00 hours). During this period, immature and adult insects were collected, and the ambient temperature, corporal temperature and weight loss were measured. From the collected material, 73 individuals from among the immatures (larvae I, II, III, pupae) and adults of the order Diptera and Coleoptera were selected and the component of interest was evaluated by HPLC. There was a positive result in 56% of the samples from larva I to adult in all the successional stages of decomposition. This technique allowed a high sensitivity from 0.1 g or less for each sample in the quantification of Propoxur residues in larvae and adults. Samples with a weight of 0.0029 g for Diptera and 0.029 g for Coleoptera were quantified. The minimum detected concentration (0,671 ppm) was found in stadium II larvae of Calliphoridae and Muscidae dipterans and the maximum quantified concentration (229,721 ppm) was found in stadium III larvae of Lucilia eximia (Diptera: Calliphoridae), with percentages of 0.04 and 0.34, respectively.

WOLFF, M., ZAPATA, Y., MORALES, G., & BENECKE, M. (2006). Detection and quantification of Proporxur in the succession of insects of medico-legal importance. Revista Colombiana De Entomología, 32(2), 159–164.

ANÓNIMO 2006. Manejo ecológico de plagas: Los plaguicidas y la salud. Sitio web: pirineosjuan/ plaguicidasalud.html. Fecha último acceso: 21 febrero 2006.

BOUREL, B.; HÉDOUVER, L.M.; BÉCART, A.; TOURNEL, G.; DEVEAUX, M.; GOSSET, D. 1999. Effects of morphine in decomposing bodies on the development of Lucilia sericata (Diptera: Calliphoridae). Journal Forensic Science 44 (2): 354-358.

BOUREL, B.; TOURNEL, G.; HÉDOUIN, V.; DEVEAUX, M.; BÉCART, A.; DEVEAUX, M.; GOFF, M. L.; GOSSET, D. 2001. Morphine extraction in necrophagous insects remains for determining antemortem

opiate intoxication”. Forensic Sciences International 120 (1-2): 127-131.

CASTILLO MIRALBES, M. 2001. Artrópodos presentes en carroña de cerdos en la comarca de La Litera (Huesca). Boletín S.E.A 28: 133-140.

ESPINAL, L.S. 1985. Geografía ecológica del Departamento de Antioquia: zonas de vida, formaciones vegetales. Revista Facultad Nacional de Agronomía Medellín, Universidad Nacional de Colombia 38: 1-104.

FAO. 1985. Prevención de pérdidas de alimentos poscosecha. Manual de Capacitación. Roma. Italia. 250 p. Sitio web: http:// Fecha último acceso: 19 octubre 2006.

GAGLIANO-CANDELA, R; AVENTAGGIATO, L. 2001. The detection of toxic substances in entomological specimens. International Journal of Legal Medicine 114: 197-203.

GOFF, M. L.; OMORI, A. I; GOODBROD, J. R. 1989. Effect of cocaine in tissues on the rate of development of Boettcherisca peregrina (Diptera: Sarcophagidae). Journal of Medical Entomology 26: 91-93.

GOFF, M. L.; BROWN, W. A.; HEWADIKARAM, K. A.; AMORI, A. I. 1991. Effects of heroin in decomposing tissues on the development rate of Boettcherisca peregrina (Diptera: Sarcophagidae) and implications of this effect on estimations of postmortem intervals using arthropod development patterns. Journal of Forensic Science 36: 537-542.

GOFF, M. L.; BROWN, W. A.; AMORI, A. I.; LA POINTE, D. A. 1993. Preliminary observations of the effects of amitriptyline in decomposing tissues on the development of Parasarcophaga ruficornis (Diptera: Sarcophagidae) and implications of this effect to estimation of postmortem interval. Journal of Forensic Science 38: 316-322.

GOFF, M. L.; MILLER, M. L.; PAULSON, J. D.; LORD, W. D.; OMARI, A. I. 1997. Effects of 3,4-methylenedioxymethamphetamine in decomposing tissues on the development of Parasarcophaga ruficornis (Diptera: Sarcophagidae) and detection of the drug in postmortem blood, liver, and puparia. Journal of Forensic Science 42 (2): 276-280.

GOFF, M. L.; LORD, W. L. 2001. Entomotoxicology: insects as toxicological indicators and impact of drugs and toxins on insect development. p. 331-340. In: Byrd, J.H; Castner, J.H. (eds.). Forensic entomology. CRC Press, Boca Raton.

HÉDOUIN, V.; BOUREL, B.; BÉCART, A.; TOURNEL, G.; DEVEAUX, M.; PHARM, D.; GOFF, M. L; GOSSET, D. 2001. Determination of drug levels in larvae of Protophormia terranovae and Calliphora vicina (Diptera: Calliphoridae) reared on rabbit carcasses containing morphine. Journal of Forensic Science 46 (1): 12-14.

INSTITUTO GEOGRÁFICO AGUSTIN CODAZZI. 1997. Zonas de vida o formación vegetal de Colombia, explicativo sobre el mapa ecológico, Bogotá. 235 p.

INTRONA, F.; LODICO, C; CAPLAN, Y. H; SMIALEK, J. E. 1990. Opiate análisis in cadaveric blowfly larvae as an indicador of narcotic intoxication. Journal of Forensic Science 35: 118-122.

INTRONA, F.; CAMPOBASSO, C. P.; GOFF, M. L. 2001. Entomotoxicology. Forensic Sciences International 120 (1-2): 42-47.

SCHOENLY, K.; GOFF, M. L.; WELL, J. D.; LORD, W. D. 1996. Quantifying statistical uncertainty in succesion based entomological estimates of the postmortem. Interval in death scene investigation: a simulation study. American Entomologist 42: 106-112.

SHALABY, O. A.; CARVALHO D. L, M.; GOFF, M. L. 2000. Comparison of Patterns of Descomposition in a Hangin Carcass and a Carcass in Contact with Soil in a Xerophytic Habitat on the Island of Oauhu, Hawaii. Journal of Forensic Science 45 (6): 1267-1273.

SMITH, K. G. 1986. A Manual of Forensic Entomology. Department of Entomology, British Museum. London. 205 p.

TIMMS, R. F. 1994. Determinación del Tiempo de Muerte en Cadáveres Putrefactos, Momificados y Saponificados. Medicina Legal de Costa Rica 10 (2): 17-21.

WOLFF, M.; BUILES, A.; ZAPATA, G; MORALES, G; BENECKE, M. 2004. Detection of Parathion (0,0-diethyl 0 (4-nitrophenyl) phosphorothioate) by HPLC in insects of forensic importante in Medellín, Colombia. Aggrawal‘s Internet Journal of Forensic Medicine and Toxicology 5 (1): 6-11.