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The black plantain weevil Cosmopolites sordidus is one of the pests that most severely damages this crop. Entomopathogenic nematodes (EN) of the genera Steinernema and Heterorhabditis are biological control agents of the insect with the possibility of being incorporated into IPM programs. The objective of this study was to evaluate the virulence of Steinernema carpocapsae All Strain and Heterorhabditis bacteriophora on adults and last instar larvae of the weevil. The bioassay used was individual infection in multiple well plates with paper filter in concentrations of 10, 100, and 1.000 infective juveniles (IJ)/25 l. Every 12 h the number of dead individuals per plate was recorded up to a maximum of 120 h for larvae and 228 h for adults. The cadavers were put in a drying chamber (development of the nematode inside the insect) and later in a “white chamber” (emergence of IJ). The variables studied were mortality of the stages, IJ multiplication and emergence duration. Under the conditions evaluated, both adults and larvae were susceptible to the attack of both nematodes, responding differentially to the increase of dose. Typical symptoms of infection and multiplication in larvae were observed for the nematode species evaluated. Conditions such as the limited niche of the insect and high humidity in plantain corms that are favorable for nematode survival, as well as the high mortality registered with low IJ concentrations and the capacity to develop especially in the larvae, make these agents promising tools for the control of the pest in the field.

SEPÚLVEDA-CANO, P. A., LÓPEZ-NÚÑEZ, J. C., & SOTO-GIRALDO, A. (2008). Effect of two enthomopathogenic nematodes on Cosmopolites sordidus (Coleoptera: Dryophthoridae). Revista Colombiana De Entomología, 34(1), 62–67. https://doi.org/10.25100/socolen.v34i1.9252

BEDDING, R. A.; MILLER, L. A. 1981. Use of a nematode, Heterorhabditis heliothidis, to control black vine weevil, Otiorhynchus sulcatus, in potted plants. Annals of Applied Biology 99: 211216.

CASTRILLÓN, C.; HERRERA, J. G. 1986. Los picudos negro y rayado del plátano y banano. Revista ICA Informa, abril-mayo junio: 11-14.

CASTRILLÓN, C. 1987. Reconocimiento del Picudo Negro (Cosmopolites sordidus Germar) del Plátano en el Departamento del Quindío. Revista ICA Informa, Manizales. Abril-Mayo-Junio: 16-21.

CASTRILLÓN, C. 2000. Distribución de las Especies de Picudo del Plátano y Evaluación de sus Entomopatógenos Nativos en el Departamento de Risaralda. CORPOICA-Comité de Cafeteros de Risaralda-UMATA Departamento de Risaralda. Manizales. 72 p.

CERDA, H.; LÓPEZ, A.; SANOJA, O.; SÁNCHEZ, P.; JAFFÉ, K. 1996. Atracción olfativa de Cosmopolites sordidus Germar (1824) (Coleoptera: Curculionidae) estimulado por volátiles originados en musáceas de distintas edades y variedades genómicas. Agronomía Tropical 46 (4): 413-429.

CHYZIK, R.; GLAZER, I.; KLEIN, M. 1996. Virulence and efficacy of different entomopathogenic nematode species against western flower thrips (Frankliniella occidentalis). Phytoparasitica 24 (2): 103-110.

DORSCHNER, K. W.; AGUDELO-SILVA, F.; BAIRD, C. R. 1989. Use of heterorhabditid and steinernematid nematodes to control black vine weevils in hop. Florida Entomologist 72: 544-556.

GARCÍA ROA, F.; GÓMEZ, J.E.; BELALCAZAR, S. 1994. Manejo biológico de Cosmopolites sordidus (Germar) en plátano.Reunión de la Asociación para la Cooperación en Investigación de Banano en el Caribe y en América Tropical. Memorias XI reunión en San José, Costa Rica. 13-18 de febrero.

GILL, S. A.; RAUPP, M. J. 1997. Evaluation of biological and chemical applications for control of iris borer. Journal Environmental Horticulture 15: 108-110.

GOITÍA, W.; CERDA, H. 1998. Hormigas y otros insectos asociados a musáceas y su relación con Cosmopolites sordidus Germar (Coleoptera: Curculionidae). Agronomía Tropical 48 (2): 209-224.

GOLD, C. S. 2000. Biology and integrated pest management of banana weevil Cosmopolites sordidus (Germar), pp. 28-33. En: Molina, A. B.; Roa, V. N.; Maghuyop, M. A. G. (eds.). Advancing banana and plantain R&D in Asia and the Pacific. Vol. 10. Proceedings of the 10th INIBAP-ASPNET Regional Advisory Commitee meeting held an Bankok, 2000/11/10-11, INIBAPASPNET, Los Baños.

GOLD, C. S.; MESSIAEN, S. 2000. El picudo negro del banano Cosmopolites sordidus. Plagas de Musa, Hoja Divulgativa 4: 1-4.

GRIFFIN, C. T.; BOEMARE, N. E.; LEWIS, E. E. 2005. Biology and Behaviour, pp. 47-64. En: Grewal, P. S.; Ehlers, R. U. Capítulo 2 Nematodes as biocontrol agents. Shapiro-Ilan, D.I. Eds. CABI Publishing. Oxfordshire. U. K.

KAYA, H. K.; KOPPENHÖFER, A. 1996. Effects of microbial and other antagonistic organism and competition in entomopathogenic nematodes. Biocontrol Science and Technology 6: 357-371.

KAYA, H.; STOCK, S. 1997. Techniques in insect nematology, pp. 281-324. En: Lacey, L. A. (ed.). Manual of techniques in insect pathology. Biological techniques. Academic Press, Inc. San Diego. Chap. 6.

KAYA, H. K.; AGUILERA, M. M.; ALUMAI, A.; CHOO, H. Y.; TORRE, M. de la; FODOR, A.; GANGULY, S.; HAZIR, S.; LAKATOS, T.; PYE, A.; WILSON, M.; YAMANAKA, S.; YANG, H.; EHLERS, R-U. 2006. Status of entomopathogenic nematodes and their symbiotic bacteria from selected countries or regions of the world. Biological Control 38: 134-155.

LEITE, L. G.; MACHADO, L. A.; GOULART, R. M.; TAVARES F. M.; BATISTA FILHO, A. 2005. Screening of entomopathogenic nematodes (Nemata: Rhabditida) and the efficiency of Heterorhabditis sp. against the Sugarcane Root Spittlebug Mahanarva fimbriolata (Fabr.) (Hemiptera: Cercopidae). Neotropical Entomology 34 (5): 785-790.

LÓPEZ-NÚÑEZ, J. C.; CANO, L.; GÓNGORA, C.; STOCK, P. 2007. Diversity and evolutionary relationships of entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) from the Central Andean region of Colombia. Nematology 9 (3): 333-341.

MERCHÁN, V. M. 1998. Manejo de problemas fitosanitarios del cultivo del plátano en la zona central cafetera, pp. 177-191. En: Giraldo, M. J.; Belalcazar, S. I.; Cayón, D.; Botero, R. G. (eds.). Memorias seminario internacional sobre producción de plátano. Armenia, Quindío. CORPOICA Eje Cafetero, Universidad del Quindío. 313 p.

NARDON, P.; LOUIS, C.; NICOLAS, G.; KERMARREC, A. 1985. Mise en evidencé et étude des bactéries symbiotiques chez deux charançons parasites du bananier: Cosmopolites sordidus, Metamasius hemipterus (L.) (Col. Curculionidae). Annales de la Société Entomologique de France 21: 245-258.

RAMOS-RODRÍGUEZ, O.; CAMPBELL, J. F.; RAMASWAMY, S. B. 2006. Pathogenicity of three species of entomopathogenic nematodes to some major stored-product insect pests. Journal of Stored Products Research 42: 241–252.

RENN, N. 1998. Routes of penetration of the entomopathogenic nematode Steinernema feltiae attacking larval and adult houseflies (Musca domestica). Journal of Invertebrate Pathology 72: 281287.

ROSALES, L. C.; SUÁREZ, Z. 1998. Nematodos entomopatógenos como posibles agentes de control del gorgojo negro del plátano Cosmopolites sordidus (Germar, 1824) (Coleoptera: Curculionidae). Boletín Entomología Venezolana 13 (2): 123140.

SÁENZ, A. 2001. Los nematodos entomopatógenos: actualidad y perspectivas. Seminario regional en control biológico. Memo rias primer seminario regional en control biológico. Santa Rosa de Cabal: Corporación Universitaria Santa Rosa de Cabal.

TREVERROW, L. N.; BEDDING, R. 1993. Development of a system for the control of the banana weevil borer, Cosmopolites sordidus with entomopathogenic nematodes, pp. 41-47. En: Bedding, R.; Akhurst, R.; Kaya, H. K. (eds.). Nematodes and the biological control of pest. Melbourne Australia..

WANG, Y.; GAUGLER, H. 1998. Host and penetration site location by entomopathogenic nematodes against Japanese beetle larvae. Journal of Invertebrate Pathology 72: 313-318.

WHITE, G. F. 1927. A method for obtaining infective nematode larvae from cultures. Science 66: 302-303.

WOUTS, W. M. 1991. Steinernema (Neoaplectana) and Heterorhabditis species, pp. 855-897. In: Nickle, W. R. (ed.). Manual of agricultural agricultural nematology. New York: Marcel Dekker.